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Screening of Antagonistic Actinomyces against Soft Rot of Amorphophallus konjac and Its Antibacterial Activity

Plant Diseases and Pests 2010年第5期1卷 30-33页

作者：叶晶龙潘虹廖甜甜李金鞠刘海军王钧乐超银三峡大学生物技术研究中心湖北宜昌443002 三峡大学校医院湖北宜昌443002

[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture *** SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,*** fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial *** antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was *** diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of *** was obtained.

关键词： Soft rot of A.konjac Antagonistic actinomycetes confrontation culture Antibacterial activity

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The Interactions Between Some Commonly Antagonistic Microbes in Tobacco Fields of Luoyang

The Interactions Between Some Commonly Antagonistic Microbes...

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中国植物病理学会2016年学术年会

作者：丁玥琪 SONG Xi-le 康业斌 College of Forestry Henan University of Science and Technology

In order to screen out the antagonistic microorganisms with benign symbiosis and enhance the control effects of tobacco soil-borne diseases by using multiple biocontrol strains,seventeen species of antagonistic microorganisms（mainly included 6 species of Trichoderma,4 species of bacteria and 7 species of actinomycetes）,which were screened and proved that have strong inhibition effects on common tobacco root pathogenic fungus from tobacco growing soil of Luoyang,were used as the research object of this experiment on the basis of previous *** this test,we measured the interactions between these antagonistic microorganisms by means of a method called improved confrontation *** mainly results obtained are as follows;（1） The confront culture results of Trichoderma strains showed that:most of Trichoderma strains ghave inhibition effects with each *** them,T1,T2 and T3 are selected for their well compatibilities,which suggested that these three species of strains have symbiotic *** inhibition effects are usually expressed as the competition of growth space,such as the impaction of aerial mycelium growth and sporulation *** the same time,there was a certain relationship between the inhibition effect and the mycelial growth rate.（2） The experimental results of the compatibilities between four tested antagonistic bacterial strains multi-performance to be *** them,the mutual inhibition rate of compatible combination B1-B2 and B3-B4 below 20%,which showed up complete compatibilities and could be used as mixed objects;however,as incompatible combination B3 has strong inhibitory effects on B1,whose inhibition rate could reach 100%and can not be mixed.（3） The confrontation results between seven speices of antagonistic actinomycetes showed that there were some inhibitory effects on different *** interactions between antagonistic actinomycetes were often expressed through influencing hyphae growth as well as changing the c

关键词： Antagonistic microbes Interactions confrontation culture

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