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Adult cannibalism in an oligophagous herbivore, the colorado potato beetle

Insect Science 2017年第2期24卷 295-302页

作者： Everett Booth Andrei Alyokhin Sarah Pinatti School of Biology and Ecology University of Maine Orono Maine United States Department of Agriculture Animal and Plant Health Inspection Service Center for Plant Health Science and Technology Buzzards Bay Massachusetts USA

Cannibalism, or intraspecific predation, can play a major role in changing individual fitness and population processes. In insects, cannibalism frequently occurs across life stages, with cannibals consuming a smaller or more vulnerable stage. Predation of adult insects on one another is considered to be uncommon. We investigated adult cannibalism in the colorado potato beetle, Leptinotarsa decemlineata （Say）, which is an oligophagous herbivore specializing on plants in family Solanaceae, and an important agricultural pest. Under laboratory conditions, starvation and crowding encouraged teneral adults to feed upon each other, which reduced their weight loss during the period of starvation. However, pupae were attacked and consumed before adults. Injured beetles had a higher probability of being cannibalized than intact beetles. Males were more frequently attacked than females, but that appeared to be a function of their smaller size rather than other gender-specific traits. Cannibalizing eggs at a larval stage did not affect beetle propensity to cannibalize adults at an adult stage. When given a choice between conspecific adults and mealworrns, the beetles preferred to eat conspecifics. Cannibalistic behavior, including adult cannibalism, could be important for population persistence in this species.

关键词： cannibalism colorado potato beetle Leptinotarsa decemlineata life boatstrategy oligophagous herbivore

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Genetic relationships of introduced colorado potato beetle Leptinotarsa decemlineata populations in Xinjiang, China

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Insect Science 2013年第5期20卷 643-654页

作者： Jing-Jie Zhang Juan Yang Ying-Chao Li Ning Liu Run-Zhi Zhang Key Laboratory of Zoological Systematics and Evolution Institute of Zoology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences Beijing College of Agriculture Guangxi University Nanning Beijing Forestry University State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

The colorado potato beetle （CPB）, Leptinotarsa decemlineata （Say）, is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 105 km2 in Xinjiang Uygur Autonomous Region. To better understand genetic variation and migration patterns, we used seven polymorphic microsatellite loci to elucidate the genetic relationships and gene flow among 10 CPB populations across Xinjiang. （i） Overall low levels of genetic diversity were detected on the entire population in Xinjiang but most of the diversity was retained among populations during invasion. （ii） The mean pairwise FST was low （0.071 ± 0.043） among populations. The genetic differentiation was little （pairwise FST 0.038 ± 0.016） between the five interior populations （Wusu, Urumqi, Jimsar, Qitai and Mulei） and Tacheng population. The six populations might come from the same genetic group via Bayesian clustering and were closely related on a neighbor-joining tree. Combining the history data, the five interior populations may have originated from Tacheng. （iii） Gene flow was frequent, especially among the five interior populations. Individuals from the interior populations could be assigned to Tacheng at higher probabilities （means 0.518 ± 0.127） than vice versa （means 0.328 ± 0.074）, suggesting that the beetle population has spread from the border to the interior in Xinjiang.

关键词： China colorado potato beetle genetic variation invasive pest Leptinotarsa decemlineata Xinjiang

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Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters colorado potato beetle larval development

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Insect Science 2017年第5期24卷 768-780页

作者： Aleksandar Cingel Jelena Savic Jelica Lazarevic Tatjana Cosic Martin Raspor Ann Smigocki Slavica Ninkovic Institute for Biological Research 'Sinisa Stankovic' University of Belgrade Bulevar despota Stefana 142 Belgrade Serbia USDA-ARS Molecular Plant Pathology Laboratory 10300 Baltimore Avenue Beltsville Maryland USA

colorado potato beetle （CPB; Leptinotarsa decemlineata Say, Coleoptera： Chrysomelidae） has shown a remarkable adaptability to a variety of control measures. Although oryzacystatin I and II （OCI and OCII） have potential in controlling pests that use cysteine proteinases for food digestion, expression of a single OC gene in potato exhibited a minimal or no effect on CPB fitness traits. The aim of this study was to examine the effect of coexpressed OCI and OCII in potato （Solanum tuberosum L.） cultivars Desiree, Draga6evka and Jelica on CPB larvae. Growth parameters, consumption rates and food utilization, as well as activity of proteases of CPB larvae were assayed. Second and third instar larvae fed on transformed leaves molted earlier and had higher relative growth and consumption rates than larvae fed on nontransformed leaves, while efficiency of food utilization was unaffected. In contrast, fourth instar maximum weight gain and amount of leaves consumed were about 20% lower for the larvae fed on transgenic potato. Analysis of total protease activity of third instar larvae revealed reduction in overall proteolytic activity measured by azocasein hydrolysis, accompanied with inhibition ofcysteine proteinase activity 24 h after ingestion of potato leaves expressing OCI and OCII. However, after long-term feeding on transformed leaves proteolytic activities of larvae became similar to the controls. Although feeding on OCI/OCII leaves did not affect larval survival, coexpression of OC genes reduced the development time and thus significantly decreased plant damage caused by CPB larvae.

关键词： colorado potato beetle gene stacking oryzacystatin I and II proteinase inhibitors Solanum tuberosum L,

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Genetic structure of the invasive colorado potato beetle Leptinotarsa decemlineata populations in China

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Journal of Integrative Agriculture 2020年第2期19卷 350-359页

作者： YANG Fang-yuan GUO Jian-jun LIU Ning ZHANG Run-zhi Institute of Entomology Guizhou UniversityGuiyang 550025P.R.China Institute of Zoology Chinese Academy of SciencesBeijing 100101P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China

The colorado potato beetle(CPB),Leptinotarsa decemlineata Say,is an infamous invasive species worldwide.It was first found in Xinjiang Uygur Autonomous Region of China in 1993 and spread to Northeast China in 2013.To better understand the genetic structure and the diffusion path of their populations in China,we used nine polymorphic microsatellite loci to elucidate the genetic diversity,genetic structure and gene flow among nine CPB populations across Xinjiang and Northeast China.The results show that:(1)Two genetically separated clusters were identified by phylogenetic tree,principal coordinate analysis(PCoA)and Bayesian cluster method.Cluster one contained populations from Xinjiang,China.Cluster two contained populations from Northeast China.A genetic differentiation existed between the two clusters.(2)Three populations in Northeast China hold an obvious genetic differentiation according to the phylogenetic tree and PCoA,indicating that multiple introductions may occur in Northeast China.(3)The Altay population in Xinjiang showed a closer genetic relationship with the populations in Northeast China which may be due to the fact that they collectively originated in neighboring Russia.(4)Among all populations,Mulei and Wusu had obvious gene migrations from Tacheng,indicating that the inland populations are most likely to originate from Tacheng,Xinjiang.

关键词： colorado potato beetle genetic variation alien invasive species microsatellite marker

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RNA interference in colorado potato beetle(Leptinotarsa decemlineata): A potential strategy for pest control

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Journal of Integrative Agriculture 2020年第2期19卷 428-437页

作者： MA Mei-qi HE Wan-wan XU Shi-jing XU Le-tian ZHANG Jiang State Key Laboratory of Biocatalysis and Enzyme Engineering College of Life SciencesHubei University

colorado potato beetle(CPB),Leptinotarsa decemlineata,is a notorious destructive pest that mainly feeds on the leaves of potato and several other solanaceous plants.CPB is widely recognized for its adaptation to a remarkable variety of host plants and diverse climates,and its high resistance to insecticides and Bacillus thuringiensis toxins.RNA interference(RNAi)is a sequence-specific,endogenous gene silencing mechanism evoked by small RNA molecules that is used as a robust tool for virus and pest control.RNAi has been extensively tested for CPB management by employing various target genes and delivery methods.This article reviews the screening of RNAi target genes,efficient RNAi delivery systems,and factors affecting RNAi efficiency in CPB,which may help understand the mechanisms of RNAi and its application in CPB control strategy.

关键词： colorado potato beetle double-stranded RNA pest control RNA interference RNAi delivery system

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Plastid-mediated RNA Interference for Control of colorado potato beetle

Plastid-mediated RNA Interference for Control of Colorado po...

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第五届全国入侵生物学大会——入侵生物与生态安全

作者： Jiang Zhang College of Life Sciences Hubei University

Plastid(chloroplast) genetic engineering has a great promise in plant biotechnology.It has several attractive advantages such as high-level of transgene expression owing to the polyploidy of the plastid genetic system,transgene containment via maternal inheritance and absence of gene silencing and pleiotropic effects.It has been demonstrated that double-stranded RNAs(ds RNAs) targeted against essential genes can trigger a lethal RNAi response in insect pests.Previously,we found chloroplasts can be capable of stably accumulating long dsRNAs,in which case dsRNA expression from the plastid genome provide better protection against colorado potato beetle(CPB),an invasive and agricultural pest in China,than dsRNA expression from the nuclear genome.We found that long dsRNAs can be stably produced in chloroplasts,and encaped from the cleavage by Dicers.Interestingly,we found that accumulation levels of dsRNA in plastids varied from 0.05% to 0.4% of total RNA and seems depend on the length and GC content of dsRNA.To comprehensive understanding of main factor affecting the stability of dsRNA in plastids,we have expressed a series of length of dsRNA in plastids and showed that the length of dsRNA can determine the dsRNA stability in plastids.Moreover,the efficiency of RNAi against CPB can be enhanced by the expression of suitable length of dsRNA in plastids.This study here will faciliate the development of RNAi-based technology for the pest control in the near future.

关键词： Crop protection colorado potato beetle Plastid genetic engineering RNAi

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