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Chinese Journal of Oceanology and Limnology 2013年第4期31卷 796-802页

作者：阳振孔繁翔 State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of Sciences

colony morphology is important for Microcystis to sustain a competitive advantage in eutrophic lakes. The mechanism of colony formation in Microcystis is currently unclear. Extracellular polysaccharide （EPS） has been reported to play an important role in cell aggregate formation of some phytoplankton. Microcystis aeruginosa was cultivated under varied abiotic conditions, including different nutrient, light, and temperature conditions, to investigate their effects on EPS production and morphological change. The results show that nutrient concentration and light intensity have great effects on EPS production in M. aeruginosa. There was a considerable increase in EPS production after M. aeruginosa was cultivated in adjusted culture conditions similar to those present in the field （28.9 mg C/L, 1.98 mg N/L, 0.65 mg P/L, light intensity： 100 μtmol/（m2.s））. These results indicate that abiotic factors might be one of the triggers for colony formation in Microcystis.

关键词： Microcystis colony formation abiotic factors cell aggregates

Key physiological traits and chemical properties of extracellular polymeric substances determining colony formation in a cyanobacterium

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Journal of Oceanology and Limnology 2022年第5期40卷 1720-1731页

作者： Zhipeng DUAN Xiao TAN Qingfei ZENG Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Ministry of EducationCollege of EnvironmentHohai UniversityNanjing 210098China College of Hydrology and Water Resources Hohai UniversityNanjing 210098China State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China

colony formation of cyanobacteria is crucial for the formation of surface blooms in ***,the underlying mechanisms of colony formation involving in physiological and cell surface characteristics remain to not well be *** cyanobacterial Microcystis strains(including both unicellular and colonial ones)were employed to estimate the influences of their physiological traits and the composition of extracellular polymeric substances(EPS)on colony or aggregate *** show that raising the number of the photosynthetic reaction center and light-harvesting antenna in the PSII and reducing the growth rate were the major physiological strategies of Microcystis to produce excess EPS enhancing colony *** bound EPS(T-EPS)was responsible for colony formation,which approximately accounted for 50%of the total amount of *** fluorescent components(protein-,tryptophan-,and tyrosine-like components and two humic-like components)were found in the T-EPS,although the amounts of these components varied with ***,colonial strains contained much higher tyrosine-like substances than unicellular *** suggest that tyrosine-like substances might serve as a crosslinking agent to connect other polymers in EPS(e.g.,proteins or polysaccharides)for colony *** findings identified key physiological traits and chemical components of EPS for colony formation in Microcystis,which can contribute to a better understanding on the formation of Microcystis blooms.

关键词： colony formation physiological properties extracellular polymeric substances(EPS)composition cyanobacterial blooms

Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

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World Journal of Gastroenterology 2009年第40期15卷 5035-5043页

作者： Qing Ye Bo Feng Yuan-Fei Peng Xue-Hua Chen Qu Cai Bei-Qin Yu Liang-Hui Li Ming-Yuan Qiu Bing-Ya Liu Min-Hua Zheng Department of General Surgery Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai Minimally Invasive Surgery Center Shanghai 200025 China Shanghai Institute of Digestive Surgery Shanghai 200025 China

AIM: To investigate the expression pattern of γ-synuclein in colorectal cancer (CRC) tissues, and to study the effects of γ-synuclein on CRC cell line HCT116 biological features in ***: The expression pattern of γ-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between γ-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA (siRNA) targeting γ-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS: The expression of γ-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the γ-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of γ-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 ***: Increased expression of γ-synuclein in CRC tissues and the biological effects of reduced γ-synuclein expression on HCT116 cells suggest that γ-synuclein may play a positive role in the progression of CRC.

关键词： γ-synuclein Colorectal cancer Expression Cell proliferation colony formation Migration Invasion

ST13,a proliferation regulator,inhibits growth and migration of colorectal cancer cell lines

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第11期13卷 884-893页

作者： Rui BAI Zhong SHI Jia-wei ZHANG Dan LI Yong-liang ZHU Shu ZHENG Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education Key Laboratory of Molecular Biology in Medical Sciences Zhejiang Province China) the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Gastroenterology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Background and objective:ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells. Methods:The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells. Results: Lentivirus-mediated overexpression of ST13 in CRC cells in-hibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo. Conclusions:ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.

关键词： Colorectal cancer ST13 Proliferation colony formation Cell cycle Migration

Holotransferrin Enhances Selective Anticancer Activity of Artemisinin against Human Hepatocellular Carcinoma Cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2013年第6期33卷 862-865页

作者：邓小荣刘朝霞刘峰潘雷余和平姜进平张建军刘立喻军 Department of General Surgery Nanshan Affiliated Hospital of Guangdong Medical College Department of Obstetrics and Gynecology Nanshan Affiliated Hospital of Guangdong Medical College Department of General Surgery First Affiliated Hospital of Nanchang University Department of General Surgery Qihe People's Hospital Department of General Surgery Wuhan Pu'ai Hospital

Artemisinin, also termed qinghaosu, is extracted from the traditional Chinese medicine ar- temesia annua L. （the blue-green herb） in the early 1970s, which has been confirmed for effectively treating malaria, Additionally, emerging data prove that artemisinin exhibits anti-cancer effects against many types of cancers such as leukemia, melanoma, etc. Artemisinin becomes cytotoxic in the presence of ferrous iron. Since iron influx is high in cancer cells, artemisinin and its analogs selectively kill can- cer cells with increased intracellular iron concentrations. This study is aimed to investigate the selective inhibitory effects of artemisinin on SMMC-7721 cells in vitro and determine the effect of holotransfer- fin, which increases the concentration of ferrous iron in cancer cells, combined with artemisinin on the anticancer activity. MTT assay was used for assessing the proliferation of SMMC-7721 cells treated with artemisinin. The induction of apoptosis and inhibition of colony formation in SMMC-7721 cells treated with artemisinin were determined by TdT-mediated dUTP nick end labeling （TUNEL） and col- ony formation assay, respectively. The results showed that artemisinin at various concentrations signifi- cantly inhibited growth, colony formation and cell viability of SMMC-7721 cells （P〈0.05）, likely due to induction of apoptosis of SMMC-7721 cells. Of interest, it was found that incubation of artemisinin combined with holotransferrin sensitized the growth inhibitory effect of artemisinin on SMMC-7721 cells （P〈0.01）. Our data suggest that treatment with artemisinin leads to inhibition of viability and pro- liferation, and apoptosis of SMMC-7721 ceils. Furthermore, we observed that holotransferrin signifi- cantly enhanced the anti-cancer activity of artemisinin. This study may provide a potential therapeutic choice for liver cancer.

关键词： human hepatocellular carcinoma SMMC-7721 cells artemisinin holotransferrin cell growth colony formation apoptosis

Inhibitory effect of parvovirus H-1 on the formation of colonies of human hepatoma cell line in vitro and its tumors in nude mice

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Cell Research 1994年第1期4卷 47-56页

作者： YAN SHANGJUN CHENGWU MA XIANHUA CHEN SHANHONG WAN ZUYU LUO(Physiology and Biophysics Department, Fudan University,Shanghai 200433, China) Physiology and Biophysics Department Fudan University Shanghai China

The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S *** were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.

关键词： Parvovirus H-1 human hepatoma cell line colony formation nude mice inhibitory effect