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Biological Feature of collagen-chitosan Membrane with Basic Fibroblast Growth Factor for the Culture of Human Fibroblast

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Chinese Journal of Biomedical Engineering(English Edition) 2003年第4期12卷 169-173页

作者： LI Qin-hua,ZHONG YuInstitute of biomedical engineering,Center for reproductive immunology research,Jinan university, Guangzhou 510632, China

The effect of collagen-chitosan membrane with different proportionate collagen and bFGF were investigated for culture human fibroblast. The optimum weight ratio of collagen/chitoson and bFGF were selected. Using culture human fibroblast technologies and cytotoxicity evaluated in vitro, Cell morphology was observed. Experimental results show that collagen-chitosan with bFGF promoted human fibroblast adhesion and supported cell proliferation for a long time. Furthermore collagen-chitosan membrane obviously degrade after 18d when human fibroblast was exhibited fusion spreading, compacting and stabilize. Cytotoxic to human fibroblast was revealed very low . collagen- chitosan with bFGF should be useful as a tissue engineering biomaterial scaffold for cell culture.

关键词： collagen-chitosan bFGF Human fibroblast Cell culture

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Preparation and Properties of collagen-chitosan/Glycosaminoglycans as Candidate Tissue Engineering Biomaterials

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Chinese Journal of Biomedical Engineering(English Edition) 2004年第2期13卷 47-51页

作者： LIQin-Hua HUANGYao-xiong CHENGJian-su InstituteofBiomedicalEngineering JinanUniversityGuangzhou510632China MedicalCollage JinanUniversityGuangzhou510632China

A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.

关键词： collagen-chitosan GAG Biomaterials scaffold Biodegradation in vivo

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