检索结果-南通市图书馆

Effects of Herbal Compound 861 on collagen Synthesis and Degradation in Rat Mesangial Cells Exposed to High Glucose

Chinese Journal of Integrative Medicine 2014年第3期20卷 209-215页

作者：马清张丽姚兰陈海平王宝恩 Department of Gerontology Beijing Friendship HospitalCapital Medical University

Objective： To investigate the effects of Herbal Compound 861 （Cpd 861） on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. Methods： The third to fifth passage of rat mesangial cells were exposed to high glucose and Cpd 861 at a concentration of 0.25-4.00 g/L for 24, 48 and 72 h, respectively. Benazepril （107-10s mmol/L） was selected as positive control. The methyl thiazolyl tetrazolium colorimetric assay was used to evaluate the effect of Cpd 861 on cell proliferation. After incubation with Cpd 861 at a concentration of 2.00 g/L for 48 h, the protein secretions of collagen type iv, matrix metallopeptidase 9 （MMP-9）, tissue inhibitor of metalloproteinase-1 （TIMP-1）, transforming growth factor beta 1 （TGF-131）, and hepatocyte growth factor （HGF） were detected by enzyme-linked immunosorbent assay method. And rat mesangial cells were harvested to determine MMP-9, TIMP-1, TGF-131 and HGF mRNA expression by revers^transcription polymerase chain reaction. Results： Cpd 861 inhibited cell proliferation induced by high glucose in a dose- and time-dependent manner. Compared with high glucose, collagen type iv production was decreased significantly by Cpd 861 （P〈0.01）. Cpd 861 increased the protein secretions and mRNA expressions of MMP-9 and HGF, whereas the protein secretions and mRNA expressions of TIMP-1 and TGF-131 were reduced markedly （P〈0.05）. The ratio of MMP-9 to TIMP-1 was enhanced by Cpd 861 significantly. There was no significant difference in all above-mentioned effects between Cpd 861 （2.00 g/L） and benazepril （10-5 mmol/L）. Conclusion： The anti-glomerulosclerosis mechanisms of Cpd 861 were partly attributed to its effects of inhibiting mesangial cell proliferation, decreasing collagen synthesis and enhancing collagen degradation.

关键词： Herbal Compound 861 mesangial cells collagen type iv matrix metalloproteinase cytokine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Clinical usefulness of biochemical markers of liver fibrosis in patients with nonalcoholic fatty liver disease

引用

World Journal of Gastroenterology 2005年第2期11卷 255-259页

作者： Hiroshi Sakugawa Tomofumi Nakayoshi Kasen Kobashigawa Tsuyoshi Yamashiro Tatsuji Maeshiro Satoru Miyagi Joji Shiroma Akiyo Toyama Tomokuni Nakayoshi Fukunori Kinjo Atsushi Saito First Department of Internal Medicine Faculty of MedicineSchool of MedicineUniversity of the RyukyusOkinawaJapan

AIM: Nonalcoholic steatohepatitis (NASH) is a severe form of nonalcoholic fatty liver disease (NAFLD), and progresses to the end stage of liver disease. Biochemical markers of liver fibrosis are strongly associated with the degree of histological liver fibrosis in patients with chronic liver disease. However, data are few on the usefulness of markers in NAFLD patients. The aim of this study was to identify better noninvasive predictors of hepatic fibrosis, with special focus on markers of liver fibrosis, type VI collagen 7S domain and hyaluronic acid. METHODS: One hundred and twelve patients with histologically proven NAFLD were studied. RESULTS: The histological stage of NAFLD correlated with several clinical and biochemical variables, the extent of hepatic fibrosis and the markers of liver fibrosis were relatively strong associated. The best cutoff values to detect NASH were assessed by using receiver operating characteristic analysis: type VI collagen 75 domain ≥5.0 ng/mL, hyaluronic acid ≥43 ng/mL. Both markers had a high positive predictive value: type VI collagen 7S domain, 86% and hyaluronic acid, 92%. Diagnostic accuracies of these markers were evaluated to detect severe fibrosis. Both markers showed high negative predictive values: type VI collagen 7S domain (≥5.0 ng/mL), 84% and hyaluronic acid (≥50 ng/mL), 78%, and were significantly and independently associated with the presence of NASH or severe fibrosis by logistic regression analysis. CONCLUSION: Both markers of liver fibrosis are useful in discriminating NASH from fatty liver alone or patients with severe fibrosis from patients with non-severe fibrosis.

关键词： Liver fibrosis Nonalcoholic fatty liver disease collagen type iv Hyaluronic acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Da Ding Feng Zhu Decoction in 30 Cases of Liver Fibrosis

引用

Journal of Traditional Chinese Medicine 2003年第4期23卷 251-254页

作者：李伟林王才党张君利王薇 Taizhou Municipal Hospital of Traditional Chinese Medicine Zhejiang Province 318000

To study the clinical effects of Da Ding Feng Zhu (大定风珠) Decoction on liver fibrosis. 56 patients withliver fibrosis due to chronic hepatitis B were randomly divided into a treatment group (30 cases treatedwith Da Ding Feng Zhu Decoction) and a control group (26 cases treated with colchicine). The serumlevels of hyaluronic acid (HA), procollagen Ⅲ (PC-Ⅲ), Ⅳ collagen (Ⅳ-C) and Laminin (LN) of thepatients were determined, compared and analyzed before treatment and after 3-month treatment in the twogroups. The results showed that in the treatment group, the levels of HA, PC-Ⅲ, Ⅳ-C and LN after3-month treatment were significantly lowered as compared to that before treatment (P＜0.01). In thecontrol group, only the HA level was obviously lowered (P＜0.05). There was an significant difference(P＜0.05) in PC-Ⅲ and Ⅳ-C and a very significant difference (P＜0.01) in HA after treatment between thetwo groups. It is therefore concluded that Da Ding Feng Zhu Decoction can lower serum indexes of liverfibrosis.

关键词： Phytotherapy Adult collagen type III collagen type iv Drug Combinations Drugs, Chinese Herbal Female Hepatitis B Humans Hyaluronic Acid Laminin Liver Cirrhosis Male Middle Aged

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transforming growth factor-β2 induces morphological alteration of human corneal endothelial cells in vitro

引用

International Journal of Ophthalmology(English edition) 2014年第5期7卷 759-763页

作者： Jing Wang Ting-Jun Fan Xiu-Xia Yang Shi-Min Chang College of Life Sciences Langfang Normal University Key Laboratory for Corneal Tissue Engineering College of Marine Life SciencesOcean University of China

AIM:To investigate the morphological altering effect of transforming growth factor-β2(TGF-β2) on untransfected human corneal endothelial cells(HCECs)in ***:After untransfected HCECs were treated with TGF-β2 at different concentrations, the morphology,cytoskeleton distribution, and type iv collagen expression of the cells were examined with inverted contrast light microscopy, fluorescence microscopy,immunofluorescence or Western ***:TGF-β2 at the concentration of 3-15 μg/L had obviously alterative effects on HCECs morphology in dose and time-dependent manner, and 9 μg/L was the peak concentration. TGF-β2(9 μg/L) altered HCE cell morphology after treatment for 36 h, increased the mean optical density(P collagen type iv in extracellular matrix(ECM) and induced the rearrangement of F-actin, microtubule in cytoplasm and collagen type iv in ECM after treatment for 72 h.·CONCLUTION: TGF-β2 has obviously alterative effect on the morphology of HCECs from polygonal phenotype to enlarged spindle-shaped phenotype, in dose and time-dependence manner by inducing more, elongation and alignment of F-actin, rearrangement of microtubule and larger spread area of collagen type iv.

关键词： human corneal endothelial cell transforming growth factor-β 2 F-actin microtubule collagen type iv

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Endothelin receptor antagonist combined with a calcium channel blocker attenuates renal injury in spontaneous hypertensive rats with diabetes

引用

Chinese Medical Journal 2002年第7期115卷 972-978,142页

作者：陈靖顾勇林凡杨海春朱蔚钰马骥林善锬复旦大学附属华山医院肾内科上海200040 温州医学院附一院肾内科温州325000

OBJECTivE: To investigate the effects of the mixed endothelin receptor antagonist, bosentan, combined with the long-acting calcium channel blocker, amlodipine, compared to the angiotensin-converting enzyme inhibitor, cilazapril, on the progressive renal injury in spontaneous hypertensive rats (SHR) with diabetes. METHODS: Diabetic hypertensive rats (SHR-DM) were induced by streptozotozin injected in male SHR (7-week-old),and divided into an untreated and three treated groups: 1) cilazapril treated group; 2) bosentan+amlodipine treated group; and 3) amlodipine treated group. Wistar Kyoto rats (WKY) and SHR rats served as normotensive and hypertensive control, respectively. The mean arterial blood pressure, renal function, endothelin and angiotensin II levels as well as the protein expression of renal extracellular matrix components and transforming growth factor (TGF)-beta1 were determined at the end of the 4th week. RESULTS: Mean arterial blood pressure significantly increased in SHR and SHR-DM rats compared to WKY rats. All the therapies reduced the blood pressure to normal levels. However, the enhanced urinary protein excretion, the decreased creatinine clearance as well as the increased plasma and intrarenal endothelin and angiotens in II levels were found in the untreated SHR-DM and prevented by treatment with bosentan+amlodipine and cilazapril. Similarly, these two kinds of therapies in SHR-DM abolished the overexpression of renal TGF-beta1 by Western blot analysis and reduced the accumulation of collagen type iv, laminin and fibronectin proteins by an immunochemical approach. Amlodipine monotherapy had no detectable effects on the above parameters. CONCLUSION: Bosentan combined with amlodipine can offer similar renoprotective effects on that of cilazapril and may be a potent therapy to attenuate renal injury by reducing renal protein levels of TGF-beta1 in diabetes with a hypertensive state.

关键词： Amlodipine Angiotensin II Animals Calcium Channel Blockers collagen type iv Diabetic Nephropathies Drug Therapy, Combination Hypertension Kidney Male Rats Rats, Inbred SHR Rats, Inbred WKY Receptors, Endothelin Research Support, Non-U.S. Gov't Sulfonamides Transforming Growth Factor beta

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：