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Effects of Calcium Dobesilate on Glomerulus TIMP1 and collagen ⅳ of Diabetic Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第4期25卷 416-418,426页

作者：董骏武刘晓城刘慎微李明波徐艳梅崔冰 Department of Nephrology Tongji Hospital Tongji Medical College Huazhong University of Science and Techology Wuhan 430030 China

Summary ： To observe the effects of calcium dobesilate on the expression of glomerular tissue inhibitor of metalloproteinase 1 （TIMP1）, collagen ⅳ , and ultrastrueture of glomerular basement mem- brane in diabetic rats, rats model of diabetes was established by unilateral nephreetomy and intraperitoneal injection of 1% STZ （55 mg/kg）, and rats were administered calcium dobesilate 100 mg/ kg （DD group） or distilled water （DM group） respectively. 12 weeks later, the changes in the renal uhrastrueture and ereatinine clearance rate （Cer） were examined in each group. The expression of glomerular TIMP1 and collagen ⅳ were studied by immunohistoehemieal staining. Our results showed that after 12 weeks, the Cer in DD group increased and was significantly higher than that in DM group. Electron microscopy showed that thickness of glomerular capillary basement membrane （GBM） in Group DD was less than that of DM group. No hyperplasia of collagen fibers was found, and the distance betweeh the holes of endothelial cells in DD group was not as even as that in the normal group, but more even than that of DM group, and podocyte processes was still in order. Immunohistochemical staining of glomeruli showed that expression of TIMP1 and collagen ⅳ in DD group were significantly less than those of DM group DM. It is concluded that calcium dobesilate can improve diabetic nephropathy by inhibiting the overaccumulation of collagen ⅳ and calcium dobesilate may also contribute to diabetes by inhibiting the expression of TIMP1.

关键词： diabetes mellitus ultrastructure calcium dobesilate matrix metalloprotelnase tissue inhibitor of metalloproteinase collagen ⅳ

collagen secretion screening in Drosophila supports a common secretory machinery and multiple Rab requirements

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Journal of Genetics and Genomics 2018年第6期45卷 299-313页

作者： Hongmei Ke Zhi Feng Min Liu Tianhui Sun Jianli Dai Mengqi Ma Lu-Ping Liu Jian-Quan Ni Jos Carlos Pastor-Pareja School of Life Sciences Tsinghua University School of Medicine Tsinghua University

collagens are large secreted trimeric proteins making up most of the animal extracellular *** of collagen has been a focus of interest for cell biologists in recent years because collagen trimers are too large and rigid to fit into the COPII vesicles mediating transport from the endoplasmic reticulum（ER） to the Golgi. collagen-specific mechanisms to create enlarged ER-to-Golgi transport carriers have been postulated, including cargo loading by conserved ER exit site（ERES） protein ***, we report an RNAi screening for genes involved in collagen secretion in Drosophila. In this screening, we examined distribution of GFP-tagged collagen IV in live animals and found 88 gene hits for which the knockdown produced intracellular accumulation of collagen IV in the fat body, the main source of matrix proteins in the larva. Among these hits, only two affected collagen secretion specifically：PH4 a EFB and Plod, encoding enzymes known to mediate posttranslational modification of collagen in the ER. Every other intracellular accumulation hit affected general secretion, consistent with the notion that secretion of collagen does not use a specific mode of vesicular transport, but the general secretory pathway. Included in our hits are many known players in the eukaryotic secretory machinery, like COPII and COPI components, SNAREs and Rab-GTPase regulators. Our further analysis of the involvement of Rab-GTPases in secretion shows that Rab1, Rab2 and Rab X3, are all required at ERES, each of them differentially affecting ERES morphology. Abolishing activity of all three by Rep knockdown, in contrast,led to uncoupling of ERES and Golgi. We additionally present a characterization of a screening hit we named trabuco（tbc）, encoding an ERES-localized TBC domain-containing Rab-GAP. Finally, we discuss the success of our screening in identifying secretory pathway genes in comparison to two previous secretion screenings in Drosophila S2 cells.

关键词： collagen ⅳ COPⅡ vesicles RNAi screening Secretory pathway Rab-GTPases ER exit site

未知病变类型脑血管中β-amyloid，α-actin，collagen ⅳ的免疫组织化学研究

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未知病变类型脑血管中β-amyloid，α-actin，collagen Ⅳ的免疫...

作者：张珉中国医科大学

学位级别：硕士

前言颅内血管破裂后,血液流入蛛网膜下腔时,称为蛛网膜下腔出血(Subarachnoid Hemorrhage,SAH)。SAH约占颅内出血的90%,在法医病理学鉴定中较为常见,可分为损伤性和非损伤性两大类,除严重脑外伤外,多在结构脆弱的脑血管处发生破裂出血... 详细信息

前言颅内血管破裂后,血液流入蛛网膜下腔时,称为蛛网膜下腔出血(Subarachnoid Hemorrhage,SAH)。SAH约占颅内出血的90%,在法医病理学鉴定中较为常见,可分为损伤性和非损伤性两大类,除严重脑外伤外,多在结构脆弱的脑血管处发生破裂出血。近几年,中国医科大学法医学院法医病理学教研室通过法医尸体解剖及病理检查发现部分死者蛛网膜下腔或脑皮质浅层小血管结构异常,并伴有不同程度的非外伤性SAH,病变血管正常平滑肌组织结构消失,在HE染色下血管中膜呈均质毛玻璃样粉染结构,严重者仅内皮残留,与动脉硬化、脑血管淀粉样变(Cerebral amyloid angiopathy,CAA)HE染色所见不尽相同。对导致此种病变的具体成因、病理分类尚不清楚,查阅文献尚无相关病例报道。 CAA是自发性颅内出血的第三大病因。CAA病变为脑血管的正常血管壁由均质透明、嗜伊红染色的淀粉样物质替代,淀粉样物质中沉积有大量4kD的β淀粉蛋白肽(β-amyloid,Aβ),Aβ是由蛋白酶水解切割β淀粉样前体蛋白(amyloid precursor protein,APP)产生。淀粉样物质经刚果红染色后,在偏振光显微镜下可见黄绿色双折光。CAA淀粉样物质主要沉积于软脑膜和脑皮质的中小血管,CAA的临床特点是多个脑叶(皮质或皮质下)出血,且易反复发作。Aβ通过破坏血管平滑肌细胞(vessel smooth muscle cell,VSMC)与细胞外基质的粘附,降低VSMC的活力,通过激活凋亡途径引起VSMC减少,并由淀粉样物质代替VSMC,导致血管结构薄弱,容易引发血管破裂出血。 VSMC存在于血管中膜,它是决定血管活性、血管构型及维持血管张力的重要因素。根据其结构和功能的不同可分为收缩型和增殖型两种表型。收缩型VSMC呈分化状态,不能增殖;增殖型VSMC呈未分化状态,可以从

关键词：未知病变类型脑小血管 β-amylod α-actin collagen ⅳ 免疫组织化学

Clinical Observation of the Effect of Xuesaitong Soft Capsule (血塞通软胶囊) on Post-hepatitis Fibrosis

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Chinese Journal of Integrated Traditional and Western Medicine 2005年第1期11卷 11-14页

作者：邓银泉范小芬李友第 The First Hospital Affiliated to Medical College of Zhejiang University Hangzhou (310003)

Objective: To study the effect of Xuesaitong soft capsule (血塞通软胶囊, XST) on liver fibrosis criteria in patients with post-hepatitis fibrosis. Methods: Sixty-four patients with such fibrosis were randomly divided into the treated group and control group. They were treated with XST and Dahuang Zhechong pill (大黄NFDA1虫丸) for 3 months respectively. Their liver fibrosis criteria were examined before and after treatment. Results: The levels of serum procollagen Ⅲ, hyaluronic acid, collagen ⅳ, laminin in the two groups were significantly lower ( P 0.05). Conclusion: XST could recover liver dysfunction and had anti-liver fibrosis function.

关键词： post-hepatitis fibrosis procollagen Ⅲ hyaluronic acid collagen ⅳ laminin Xuesaitong soft capsule

ARAP1/AT1R信号通路在糖尿病肾病发病机制中的作用及干预研究

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ARAP1/AT1R信号通路在糖尿病肾病发病机制中的作用及干预研究

作者：鲁伶旭中国医科大学

学位级别：硕士

目的:探讨2型糖尿病db/db小鼠及体外高糖培养肾小球的系膜细胞中,A RAP1的表达变化及与AT1R的相互调控作用,是否引起系膜细胞增殖,细胞外基质积聚,氧化应激亢进,从而导致糖尿病肾病的损伤。以及观察乌索酸对2型糖尿病db/db小鼠的肾脏保... 详细信息

目的:探讨2型糖尿病db/db小鼠及体外高糖培养肾小球的系膜细胞中,A RAP1的表达变化及与AT1R的相互调控作用,是否引起系膜细胞增殖,细胞外基质积聚,氧化应激亢进,从而导致糖尿病肾病的损伤。以及观察乌索酸对2型糖尿病db/db小鼠的肾脏保护作用以及对ARAP1和AT1R的表达的影响。研究方法:9周龄db/db小鼠(***-db/db)按照空腹体重随机分为糖尿病肾病组(db/db组,n=10)和乌索酸治疗组(db/db+0.3%UA,n=10)。同周龄db/m小鼠(***-db/+,n=10)作为对照组。每周检测各组小鼠体重;定期检测血糖;于20周龄随机的留取尿液测定尿白蛋白/尿肌酐比率。光镜下观察肾组织的病理改变;免疫组化检测肾组织内的血管紧张素II1型受体相关蛋白(ARAP1)、血管紧张素II1型受体(AT1R)、细胞外基质蛋白纤维连接蛋白(F N)的表达变化。实时定量PCR法检测血管紧张素II1型受体相关蛋白(ARAP1)、血管紧张素II1型受体(AT1R)、NADPH氧化酶4(NOX4)、NADPH氧化酶2(NOX2)、FN、IV胶原(collagen IV)mRNA的表达变化。Western印迹法检测肾皮质内血管紧张素II1型受体相关蛋白(ARAP1)、血管紧张素II1型受体(AT1R)、NADPH氧化酶4(NOX4)、NADPH氧化酶2(NOX2)、转化生长因子-β1(TGF-β1)、FN、IV胶原(collagen IV)的蛋白表达变化。将体外培养的大鼠的肾小球系膜细胞分为正常糖组(NG)、高糖组(HG)、正常糖阴性对照组、高糖阴性对照组、正常糖+ARAP1siRNA和高糖+ARAP1siR NA组;Western-blot检测各组ARAP1、AT1R、FN蛋白的表达变化。结果:各周龄db/db小鼠体重均显著高于db/m组(pcollagen IV蛋白表达显著增加(p<0.01);乌索酸治疗可显著降低肾皮质内上述指标的异常表达。体外高糖环境培养的大鼠的肾小球系膜细胞中ARAP1、AT1R及FN蛋白的表达明显升高(p<0.01),与正常糖组比。分别向正常糖及高糖组转染ARAP1siRN A,与高糖组相比抑制ARAP1的表达后,AT1R的表达降低,减少细胞外基质的积聚。结论:2型糖尿病db/db小鼠的肾皮质中ARAP1表达升高,促进AT1R的表达升高,从而增强AT1R其对血管紧张素II的敏感性进一步促进NOX4、2的表达,引起氧化应激的亢进;促进TGF-β1、FN和COIIV的表达,导致肾脏的纤维化病变。乌索酸可能通过下调ARAP1/AT1R信号通路抑制细胞外基质增多、肾脏纤维化和氧化应激反应,缓解2型糖尿病db/db小鼠肾脏损伤。体外高糖培养的大鼠的肾小球系膜细胞中ARAP1、AT1R及FN的表达显著升高。靶向抑制系膜细胞中ARAP1的表达后,减少了AT1R的表达,减轻细胞外基质分泌的产生。

关键词：糖尿病肾病乌索酸血管紧张素Ⅱ1型受体相关蛋白1(ARAP1) 血管紧张素Ⅱ1型受体(AT1R) 氧化应激 NOX2、4 TGF-β1 FN collagen ⅳ