Tocopherols, well known as vitamin E, are a group of important lipid-soluble antioxidants that play indispensable roles in living organisms. The physiological functions of tocopherols have been comprehensively charact...
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Tocopherols, well known as vitamin E, are a group of important lipid-soluble antioxidants that play indispensable roles in living organisms. The physiological functions of tocopherols have been comprehensively characterized in animals and artificial membranes;they participate in the stabilization of membranes by quenching and scavenging reactive oxygen species and free radicals, as well as protecting polyunsaturated fatty acids from lipid peroxidation. However, molecular functions of tocopherols in plants(or photosynthetic organisms) in which they are produced are less understood. This study aimed to isolate a natural tocopherol deficient mutant rtd1 in rice. The rtd1 mutant showed overall growth retardation during the whole growth period. Most of the agronomic traits of rtd1 were impaired. Map-based cloning revealed that the RTD1 gene encoded a homogentisate phytyltransferase, a key enzyme catalyzing the committed step in tocopherol biosynthesis. Tocopherols could not be detected in the mutant by high-performance liquid chromatography. RTD1 was preferentially expressed in green leafy tissues, and the protein was proved to be located in chloroplasts. Also, cold tolerance was found to be reduced in rtd1. The cold-related C-repeat-binding factor(CBF)/dehydration-responsive element-binding protein 1(DREB1) genes were significantly upregulated in rtd1 under natural growth conditions. Moreover, the rtd1 mutant showed a reduced response to gibberellin. The transcript and protein levels of the GA signaling repressor, the DELLA protein SLENDER RICE 1(SLR1) in rice increased in rtd1, which might partially explain the growth retardation of rtd1. However, GA content was not changed, suggesting a transcriptional, not posttranslational, regulation of SLR1. These findings implied that tocopherols play important roles in regulating rice growth and development.
<正>DREB transcription factors play an important role in tolerance to abiotic stress in high *** this work,two new DRE-binding protein genes MfDREB1and MfDREB1s cDNA that encoded an AP2/EREBP type transcription fact...
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<正>DREB transcription factors play an important role in tolerance to abiotic stress in high *** this work,two new DRE-binding protein genes MfDREB1and MfDREB1s cDNA that encoded an AP2/EREBP type transcription factor were isolated by RT-PCR from Medicago falcate *** analysis showed MfDREB1and MfDREB1s were almost identical except that there was a202bp fragment at the3′end of the MfDREB1s cDNA that is absent in MfDREB1cDNA.
Chrysanthemum was one of the four cut flowers in world, was also very important in China for cutting flower recent years. Winter chrysanthemum was one special population and more cold tolerance. The relative electric ...
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Chrysanthemum was one of the four cut flowers in world, was also very important in China for cutting flower recent years. Winter chrysanthemum was one special population and more cold tolerance. The relative electric conductivity (REC), content of MDA, soluble sugar and protein, SOD,POD activity and pollen viability under natural and artificial low temperature were investigated in four winter cut chrysanthemum cultivars'Hanzi', 'Hanhuang'. The logistic equation was constructed based on the relationship between REC and temperature below 0℃, then the semi-lethal temper- ature(LT50) was determined, and then compared the cold tolerance of different cultivars, different florets and different flowering by variance analysis. The main results were as follows:(1) From 15th Dec to 27th,2008, the tendency of REC, MDA content of four cultivars changed reversely compared with the temperature, while SOD,POD and content of soluble protein were highly related to the low temperature, and the soluble sugar content accumulated all the time. The four different cultivars improved the cold tolerance by regulating the activity of SOD and the contents of soluble sugar and protein.(2) The LT50 in different florets and flowering of'Hanzi'and'Hanhuang'ranged from-8.82℃~-12.94℃. When temperature from CK (14.4~16.2℃.) to 3℃, the SOD activity, soluble protein and pollen viability increased at beginning and then decreased, while MDA changed reversely, and the soluble sugar content accumulated all the time. The change appeared at 9℃to/or 6℃, and the temperature were critical temperature point of physiological barrier.The greatest germination capacity of 'Hanzi' and 'Hanhuang' appears when temperature was 12℃and 9℃, respectively. All of these showed that the pollen viability of 'Hanzi'was more easily affected by low temperature.(3) The changed of physiological and biochemical indexes suggested that the chilling tolerance of two chrysanthemum was'Hanhuang','Hanzi', in turn. Tubular f
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