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Transcription factor CabHLH035 promotes cold resistance and homeostasis of reactive oxygen species in pepper

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Horticultural Plant Journal 2024年第3期10卷 823-836页

作者： Huafeng Zhang Jiangbai Guo Xiaoqing Chen Yunyun Zhou Yingping Pei Lang Chen Saeed ul Haq Mingke Zhang Haijun Gong Rugang Chen College of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China Shaanxi Engineering Research Center for Vegetables YanglingShaanxi 712100China Department of Horticulture The University of Agriculture PeshawarPeshawarKhyber Pakhtunkhwa 25130Pakistan

Plant basic helix-loop-helix(bHLH)transcription factors(TFs)play central roles in various abiotic ***,its role in plant cold resistance is largely ***,we characterised CaNAC035 in pepper,which positively regulates tolerance to cold,salt and drought stresses ***,we identified CabHLH035,a CaNAC035-interacting protein in *** explore its functions in cold stress tolerance,we silenced the gene in pepper via virus-induced gene silencing(VIGS)and overexpressed the gene in *** results showed that CabHLH035 expression was induced by cold treatment,and silencing of CabHLH035 decreased cold stress ***,overexpression of CabHLH035 in Arabidopsis increased cold stress *** investigate homologs genes of C-repeat binding factor(CBF)pathway proteins and reactive oxygen species(ROS)marker gene expression blocking by CabHLH035,we performed yeast one-hybrid(Y1H),dual luciferase and electrophoretic mobility shift assay *** results showed that CabHLH035 bound to the region upstream of the CaCBF1A and CaAPX ***,CaCBF1A bound to the CaDHN4 *** together,our results showed that CabHLH035 plays a crucial role in cold stress tolerance and its potential as a target for breeding cold-resistant *** findings provide a basis for studying the functions and regulatory network of cold stress tolerance in pepper.

关键词： Pepper CabHLH035 Silencing Overexpression VIGS cold tolerance

Female ticks (Ixodes scapularis) infected with Borrelia burgdorferi have increased overwintering survival, with implications for tick population growth

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Insect Science 2023年第6期30卷 1798-1809页

作者： Amal El Nabbout Laura V.Ferguson Atsushi Miyashita Shelley A.Adamo Department of Biology Dalhousie UniversityHalifaxNova ScotiaCanada Department of Biology Acadia UniversityWolfvilleNova ScotiaCanada Institute of Medical Mycology Teikyo UniversityTokyoJapan Department of Psychology and Neuroscience Dalhousie UniversityNova ScotiaCanada

The tick, Ixodes scapularis, vectors pathogens such as Borrelia burgdorferi, the bacterium that causes Lyme disease. Over the last few decades I. scapularis has expanded its range, introducing a novel health threat into these areas. Warming temperatures appear to be one cause of its range expansion to the north. However, other factors are also involved. We show that unfed adult female ticks infected with B. burgdorferi have greater overwintering survival than uninfected female ticks. Locally collected adult female ticks were placed in individual microcosms and allowed to overwinter in both forest and dune grass environments. In the spring we collected the ticks and tested both dead and living ticks for B. burgdorferi DNA. Infected ticks had greater overwintering survival compared with uninfected ticks every winter for three consecutive winters in both forest and dune grass environments. We discuss the most plausible explanations for this result. The increased winter survival of adult female ticks could enhance tick population growth. Our results suggest that, in addition to climate change, B. burgdorferi infection itself may be promoting the northern range expansion of I. scapularis. Our study highlights how pathogens could work synergistically with climate change to promote host range expansion.

关键词： cold tolerance ecological immunology Lyme disease thermobiology winter

Selective footprints and genes relevant to cold adaptation and other phenotypic traits are unscrambled in the genomes of divergently selected chicken breeds

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Journal of Animal Science and Biotechnology 2023年第3期14卷 887-907页

作者： Michael N.Romanov Alexandra S.Abdelmanova Vladimir I.Fisinin Elena A.Gladyr Natalia A.Volkova Olga A.Koshkina Andrey N.Rodionov Anastasia N.Vetokh Igor V.Gusev Dmitry V.Anshakov Olga I.Stanishevskaya Arsen V.Dotsev Darren K.Griffin Natalia A.Zinovieva L.K.Ernst Federal Research Centre for Animal Husbandry DubrovitsyPodolskMoscow RegionRussia School of Biosciences University of KentCanterburyUK Federal State Budget Scientific Institution Federal Research Centre“All-Russian Poultry Research and Technological Institute”of the Russian Academy of Sciences Sergiev PosadMoscow RegionRussia Breeding and Genetic Centre“Zagorsk Experimental Breeding Farm”–Branch of the Federal Research Centre“All-Russian Poultry Research and Technological Institute”of the Russian Academy of Sciences Sergiev PosadMoscow RegionRussia Russian Research Institute of Farm Animal Genetics and Breeding–Branch of the L.K.Ernst Federal Research Centre for Animal Husbandry St.PetersburgRussia

Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture *** to diverse environments,including acclimation to harsh climatic conditions,has also left selection footprints in breed *** Using the Chicken 50K_CobbCons SNP chip,we genotyped four divergently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White *** of selective sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation *** regions of true selection signatures were identified by two or more methods or in two or more *** these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other *** them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold *** factors may be involved in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying various phenotypic traits in chicken *** include genes representing the sine qua non for adaptation to harsh *** tolerance in acclimated chicken breeds may be developed following one of few specific gene expression mechanisms or more than one overlapping response known in cold-exposed individuals,and this warrants further investigation.

关键词： Acclimation Adaptation Chicken breeds cold tolerance Divergent selection Genetic diversity Genomewide scan Phenotypic traits Selective sweeps

Development of a New cold-Tolerant Maize(Zea mays L.)Germplasm Using the ICE1 Gene from Arabidopsis thaliana

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Phyton-International Journal of Experimental Botany 2022年第8期91卷 1709-1719页

作者： Jing Qu Shuang Liu Peng Jiao Zhenzhong Jiang Jianbo Fei Shuyan Guan Yiyong Ma College of Agronomy Jilin Agricultural UniversityChangchun130118China College of Life Sciences Jilin Agricultural UniversityChangchun130118China

To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION(ICE1)expression in response to cold stress,RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector *** maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated *** the regenerated plantlets,three T1 lines were screened and identi-fied by PCR.A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize(Zea mays L.)genomes of the three T1 *** low temperature-stress conditions(4°C),the relative conductivity levels decreased by 27.51%–31.44%,the proline concentrations increased by 12.50%–17.50%,the malondialdehyde concentrations decreased by 16.78%–18.37%,and the peroxidase activities increased by 19.60%–22.89%in the T1 lines compared with those of the control.A real-time quantitative PCR analysis showed that the ICE1 gene was ectopically expressed in the roots,stems,and leaves of the T1 ***1 positively regulates the expression of the CBF genes in response to cold ***,this study showed the successful transformation of maize with the ICE1 gene,resulting in the generation of a new maize germplasm that had increased tolerance to cold stress.

关键词： Maize ICE1 cold tolerance inducer maize Arabidopsis thaliana

冬季最低温和温度季节变异对北美树木分布北界及多样性的调控研究

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Journal of Geographical Sciences 2022年第2期32卷 280-290页

作者：钱宏张扬建 Robert E.RICKLEFS 王宪礼 Research and Collections Center Illinois State Museum1011 East Ash StreetSpringfieldIL 62703USA Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchCASBeijing 100101China Department of Biology University of Missouri–St.LouisSt.LouisMO 63121USA Northern Forestry Centre Canadian Forest ServiceNatural Resources Canada5320-122nd StreetEdmontonAB T6H 3S5Canada Department of Renewable Resources University of Alberta751 General Service Building EdmontonAB T6G 2H1Canada

Biologists have considered both winter coldness and temperature seasonality as major determinants of the northern limits of plants and animals in the Northern Hemisphere,which in turn drive the well-known latitudinal diversity ***,few studies have tested which of the two climate variables is the primary *** this study,we assess whether winter coldness or temperature seasonality is more strongly associated with the northern latitudinal limits of tree species and with tree species richness in North *** species were recorded in each of 1198 quadrats of 110 km×110 km in North *** used correlation and regression analyses to assess the relationship of the latitude of the northern boundary of each species,and of species richness per quadrat,with winter coldness and temperature *** richness was analyzed within 38 longitudinal,i.e.,north-south,bands(each being>1100 km long and 110 km wide).The latitudes of the northern range limits of tree species were three times better correlated with minimum temperatures at those latitudes than with temperature *** average,minimum temperature and temperature seasonality together explained 81.5%of the variation in the northern range limits of the tree species examined,and minimum temperature uniquely explained six-fold(33.7%versus 5.8%)more of this variation than did temperature *** of tree species richness with minimum temperatures were stronger than correlations with temperature seasonality for most of the longitudinal bands *** to temperature seasonality,winter coldness is more strongly associated with species distributions at high latitudes,and is likely a more important driver of the latitudinal diversity gradient.

关键词： cold tolerance latitudinal diversity gradient minimum temperature temperature seasonality temperature variation trees tropical conservatism hypothesis

Short-term cold storage of blowfly Lucilia sericata embryos

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Insect Science 2008年第3期15卷 225-228页

作者： Bo Zhang Hideharu Numata Hideya Mitsui Shin G. Goto Graduate School of Science Osaka City University Sugimoto Sumiyoshi Osaka 558-8585Okayama University Okayama 700-8558 Japan Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama 700-8558 Japan

The developmental rate under low temperatures and cold tolerance were investigated in embryos of the blowfly Lucilia sericata. The larvae of this species are now widely used in maggot debridement therapy. Embryonic development was dependent on temperature, with a lower developmental threshold of 9.0℃. The duration of the egg stage at a rearing temperature of 25℃was 14 h, and a low temperature of 12.5℃ successfully prolonged this period to 66 h. Embryonic stages differed markedly in their cold tolerance; young embryos were less tolerant to cold than old ones. Late embryonic stages are suitable for cold storage at 5℃ and the storage for 72 h did not decrease the hatching rate by more than 50%. In the mass-rearing process required for maggot debridement therapy, either of these two simple protocols would be beneficial.

关键词： blowfly cold tolerance developmental rate egg development embryo lowtemperature Lucilia seriacta maggot debridement therapy

Adaptive associations between total body color dimorphism and climatic stress-related traits in a stenothermal circumtropical Drosophila species

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Insect Science 2012年第2期19卷 247-262页

作者： Ravi Parkash Jyoti Chahal Vineeta Sharma Kapil Dev Department of Genetics Maharshi Dayanand UniversityRohtakIndia

Low desiccation resistance of Drosophila ananassae reflects its rarity outside the humid tropics. However, the ability of this sensitive species to evolve under seasonally varying subtropical areas is largely unknown. D. ananassae flies are mostly lighter during the rainy season but darker and lighter flies occur in the autumn season in northern India. We tested the hypothesis whether seasonally varying alternative body color phenotypes of D. ananassae vary in their levels of environmental stress tolerances and mating behavior. Thus, we investigated D. ananassae flies collected during rainy and autumn seasons for changes in body melanization and their genetic basis, desiccation-related traits, cold tolerance and mating propensity. On the basis of genetic crosses, we found total body color dimorphism consistent with a single gene model in both sexes olD. ananassae. A significant increase in the frequency of the dark morph was observed during the drier autumn season, and body color phenotypes showed significant deviations from Hardy-Weinberg equilibrium, which suggests climatic selection plays a role. Resistance to desiccation as well as cold stress were two- to three-fold higher in the dark body color strain as compared with the light strain. On the basis of no-choice mating experiments, we observed significantly higher assortative matings between dark morphs under desiccation or cold stress, and between light morphs under hot or higher humidity conditions. To the best of our knowledge, this is the first report on the ecological significance of seasonally varying total body color dimorphism in a tropical species, D. ananassae.

关键词： assortative matings cold tolerance dark and the light morph desiccation resistance Drosophila ananassae seasonal adaptations

The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2017年第2期59卷 118-133页

作者： Yihao Shi Jiaying Huang Tianshu Sun Xuefei Wang Chenqi Zhu Yuxi Ai Hongya Gu State Key Laboratory for Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China

The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive （COR） genes. In this study, we obtained CRISPR/Casg-mediated loss-of-function mutants of cbf1-3. Over 3,ooo COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild- type plants after being treated at 4 ℃ for 12 h. The C-repeat （CRT） motif （5＇-CCGAC-3＇） was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBFI. These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes; less than 1[3 of the genes were regulated by one CBF, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.

关键词： CRISPR Cas9 CBF cold tolerance COR single CBF-regulated genes

Relationship between clade age and temperature for angiosperm tree species in forest communities along an elevational gradient in tropical Asia

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Journal of Plant Ecology 2017年第4期10卷 618-625页

作者： Hong Qian Research and Collections Center Illinois State Museum1011 East Ash StreetSpringfieldIL 62703USA

Aims The tropical conservatism hypothesis(TCH)links environmental tolerance,diversification,dispersal and evolutionary history in explaining why warm,wet tropical regions harbor more species than colder,drier *** TCH is considered as a dominant explanation for broad-scale patterns of species richness across the globe,such as the latitudinal diversity *** its core,the TCH predicts a positive relationship between clade age and ***,I test this prediction using a data set of angiosperm tree assemblages from *** I assembled an altitudinal gradient of 15 communities of angio-sperm trees.I calculated the mean family age(MFA)of tree species for each community.I used ordinary regression analysis and spatial autoregression analysis to determine the relationships of species richness and MFA with elevation,temperature and precipitation.I used correlation analysis to assess relationships between paired *** Findings MFA is negatively correlated with tree species richness,and increases with elevation and decreases with temperature for the alti-tudinal *** remains significantly increasing with decreas-ing temperature along the altitudinal gradient after accounting for spatial autocorrelation in a species-ordination *** negative relationship between clade age and temperature along the altitudi-nal gradient in Malesia is contrary to the TCH.

关键词： angiosperm cold tolerance community assembly altitudinal diversity gradient environmental filtering mean family age phylogenetic niche conservatism