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Antioxidant metabolic system and comparative proteomics analysis in winter turnip rape(Brassica rapa L.)under cold stress

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Oil Crop Science 2022年第2期7卷 95-102页

作者： Xiucun Zeng Li Ma Jinhai Yuan Yaping Xie Tianxia Guan Xiaoqin Wang Guotai Ma Yaozhao Xu Wancang Sun School of Life Science and Engineering/College of Agriculture and Ecological Engineering/Key Laboratory of Hexi Corridor Resources Utilization of Gansu Hexi UniversityZhangye734000China College of Agronomy Gansu Agricultural UniversityLanzhou730070China Linze County Seed Administration Zhangye734000China Crop Research Institute Gansu Academy of Agricultural SciencesLanzhou730070China

Winter turnip rape(Brassica rapa L.)is widely cultivated in winter in Northwest China,however,its cold-tolerant mechanism remains insufficiently understood.In this study,winter turnip rape cultivar Longyou 7,a cold-tolerant variety,was used as material,whose accumulation of H_(2)O_(2) and O_(2)^(·-),antioxidant enzyme activity as well as differences in protein expression based on two-dimensional gel electrophoresis(2-DE)technique under -4℃ stress were analyzed.Results showed that,production of H_(2)O_(2) and O_(2)^(·-) were increased in Longyou 7 leaves,simultaneously,SOD and POD activities were also obviously rosed up,but the activities of CAT and APX were gradually reduced with the temperature.Thirty-six differential protein spots were successfully identified between control and treatments group by using mass spectrometry analysis.Among them,4 differential protein spots were induced under cold stress,and 2 were inhibited at-4℃.Functional analysis found that these identified proteins mainly participated in photosynthesis,carbohydrate metabolism,defense,protein synthesis,enzyme activity,redox and membrane metabolism,respectively.Additionally,13 proteins'function were still unknown.In conclusion,strong antioxidant capacity and cell defense ability might play important roles in Longyou 7 response to cold stress.

关键词： Brassica rapa Accumulation of H2O2 and O2- Antioxidant enzyme activity cold stress

Genetic and lipidomic analyses reveal the key role of lipid metabolism for cold tolerance in maize

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Journal of Genetics and Genomics 2024年第3期51卷 326-337页

作者： Lei Gao Haifang Jiang Minze Li Danfeng Wang Hongtao Xiang Rong Zeng Limei Chen Xiaoyan Zhang Jianru Zuo Shuhua Yang Yiting Shi State Key Laboratory of Plant Environmental Resilience College of Biological SciencesFrontiers Science Center for Molecular Design BreedingCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China State Key Laboratory of Wheat&Maize Crop Science College of Life SciencesHenan Agricultural UniversityZhengzhouHenan 450002China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Suihua Branch of Heilongjiang Academy of Agricultural Machinery Sciences SuihuaHeilongjiang 152052China

Lipid remodeling is crucial for cold tolerance in plants.However,the precise alternations of lipidomics during cold responses remain elusive,especially in maize(Zea mays L.).In addition,the key genes responsible for cold tolerance in maize lipid metabolism have not been identified.Here,we integrate lipidomic,transcriptomic,and genetic analysis to determine the profile of lipid remodeling caused by cold stress.We find that the homeostasis of cellular lipid metabolism is essential for maintaining cold tolerance of maize.Also,we detect 210 lipid species belonging to 13 major classes,covering phospholipids,glycerides,glycolipids,and free fatty acids.Various lipid metabolites undergo specific and selective alterations in response to cold stress,especially mono-/di-unsaturated lysophosphatidic acid,lysophosphatidylcholine,phosphatidylcholine,and phosphatidylinositol,as well as polyunsaturated phosphatidic acid,monogalactosyldiacylglycerol,diacylglycerol,and triacylglycerol.In addition,we identify a subset of key enzymes,including ketoacyl-acyl-carrier protein synthase II(KAS II),acyl-carrier protein 2(ACP2),male sterility33(Ms33),and stearoyl-acyl-carrier protein desaturase 2(SAD2)involved in glycerolipid biosynthetic pathways are positive regulators of maize cold tolerance.These results reveal a comprehensive lipidomic profile during the cold response of maize and provide genetic resources for enhancing cold tolerance in crops.

关键词： Maize cold stress Lipid metabolism Transcriptome Lipidomic

Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling

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Horticultural Plant Journal 2024年第2期10卷 532-544页

作者： Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province Sanya Nanfan Research InstituteHainan UniversitySanyaHainan 572025China Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province School of HorticultureHainan UniversityHaikouHainan 570228China Hainan Yazhou Bay Seed Laboratory SanyaHainan 572025China

This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin(ME) is a dynamic molecule that helps plants cope with stress in several ways. cold stress(CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper(Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME(5 μmol · L^(-1)) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species(ROS), malondialdehyde(MDA), electrolyte leakage(EL), proline, and soluble sugars were enhanced in plants under CS. ME(5 μmol · L^(-1)) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide(H_(2)O_(2)), superoxide ion(O_(2)^(·-)), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth,improving the photosynthesis apparatus, regulating pigments, and osmolyte content.

关键词： Pepper Melatonin cold stress Antioxidant enzyme Root trait

Protein kinases in plant responses to drought, salt,and cold stress

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Journal of Integrative Plant Biology 2021年第1期63卷 53-78页

作者： Xuexue Chen Yanglin Ding Yongqing Yang Chunpeng Song Baoshan Wang Shuhua Yang Yan Guo Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop stress Biology Henan ProvinceInstitute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071001China

Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields.

关键词： CDPKs cold stress drought stress MAPK cascade RLKs salt stress Sn RKs

Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant cold stress Signaling

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Molecular Plant 2009年第1期2卷 59-72页

作者： Chun-Hai Dong Bethany K. Zolman Bonnie Bartel Byeong-ha Lee Becky Stevenson Manu Agarwal Jian-Kang Zhu Department of Botany and Plant Science 2150 Batchelor Hall University of California at Riverside Riverside CA 92521 USA Department of Biology University of Missouri-St Louis St Louis MO 63121 USA Department of Biochemistry and Cell Biology Rice University Houston TX 77005 USA Present address: Department of Cell Biology and Molecular Genetics University of Maryland College Park MD 20742 USA Present address: Department of Life Science Sogang University Seoul 121-742 South Korea

To study cold signaling, we screened for Arabidopsis mutants with altered cold-induced transcription of a firefly luciferase reporter gene driven by the CBF3 promoter （CBF3-LUC）. One mutant, chy1-10, displayed reduced cold-induction of CBF3-LUC luminescence. RNA gel blot analysis revealed that expression of endogenous CBFs also was reduced in the chyl mutant, chy1-10 mutant plants are more sensitive to freezing treatment than wild-type after cold acclimation. Both the wild-type and chyl mutant plants are sensitive to darkness-induced starvation at warm temperatures, although chyl plants are slightly more sensitive. This dark-sensitivity is suppressed by cold temperature in the wildtype but not in chyl. Constitutive CBF3 expression partially rescues the sensitivity of chyl-10 plants to dark treatment in the cold. The chyl mutant accumulates higher levels of reactive oxygen species, and application of hydrogen peroxide can reduce cold-induction of CBF3-LUC in wild-type. Map-based cloning of the gene defective in the mutant revealed a nonsense mutation in CHY1, which encodes a peroxisomal β-hydroxyisobutyryl （HIBYL）-CoA hydrolase needed for valine catabolism and fatty acid β-oxidation. Our results suggest a role for peroxisomal metabolism in cold stress signaling, and plant tolerance to cold stress and darkness-induced starvation.

关键词： cold stress signal transduction gene regulation CHY1 β-hydroxyisobutyryI-CoA hydrolase.

cold stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis

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Horticultural Plant Journal 2020年第6期6卷 439-449页

作者： Mingyue Zhao Binbin Cai Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural UniversityHefei 230036China Key Laboratory of Tea Science of Ministry of Education National Research Center of Engineering Technology for Utilization of Functional Ingredients from BotanicalsCollege of HorticultureHunan Agricultural UniversityChangsha 410128China

Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.

关键词： Camellia sinensis Eugenol Glucosyltransferase cold stress

Therapeutic effect of bone marrow mesenchymal stem cells on cold stress induced changes in the hippocampus of rats

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Neural Regeneration Research 2014年第19期9卷 1740-1744页

作者： Saravana Kumar Sampath Kumar Saraswathi Perumal Vijayaraghavan Rajagopalan Department of Anatomy Saveetha Dental College Saveetha University ChennaiTamil NaduIndia

The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold stress induced neuronal changes in hippocampal CA1 region of Wistar rats. Bone marrow mes- enchymal stem cells were isolated from a 6-week-old Wistar rat. Bone marrow from adult femora and tibia was collected and mesenchymal stem cells were cultured in minimal essential medium containing 10% heat-inactivated fetal bovine serum and were sub-cultured. Passage 3 cells were analyzed by flow cytometry for positive expression of CD44 and CD90 and negative expression of CD45. Once CD44 and CD90 positive expression was achieved, the cells were cultured again to 90% confluence for later experiments. Twenty-four rats aged 8 weeks old were randomly and evenly divided into normal control, cold water swim stress （cold stress）, cold stress ＋ PBS （intra- venous infusion）, and cold stress ＋ bone marrow mesenchymal stem cells （1 x 106; intravenous infusion） groups. The total period of study was 60 days which included 1 month stress period followed by 1 month treatment. Behavioral functional test was performed during the entire study period. After treatment, rats were sacrificed for histological studies. Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA 1 region. Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

关键词： nerve regeneration bone marrow mesenchymal stem cells hippocampus cold stress intravenous cognition neuronal cells CA1 region neural regeneration

cold stress responsive microRNAs and their targets in Musa balbisiana

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 335-345页

作者： Jingyi WANG Juhua LIU Caihong JIA Hongxia MIAO Jianbin ZHANG Zhuo WANG Biyu XU Zhiqiang JIN Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Key Laboratory of Genetic Improvement of Bananas Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences

cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although mi RNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature(5°C).A total of 69 known mi RNAs and 32 putative novel mi RNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible mi RNAs were identified through differentially expressed mi RNAs analysis. Among 43 mi RNAs belonging to 26 conserved mi RNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of21 putative novel mi RNAs with altered expression, four were downregulated and 17 upregulated. Furthermore,eight mi RNAs were validated by stem-loop q RT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive mi RNAs were predicted and categorized by function.These results provide important information for further characterization and functional analysis of cold-responsive mi RNAs in banana.

关键词： cold stress micro RNA Musa balbisiana

Functional characterization of MdMYB73 reveals its involvement in cold stress response in apple calli and Arabidopsis

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Journal of Integrative Agriculture 2017年第10期16卷 2215-2221页

作者： ZHANG Quan-yan YU Jian-qiang WANG Jia-hui HU Da-gang HAO Yu-jin State Key Laboratory of Crop Biology National Research Center for Apple Engineering and Technology Shandong Agricultural University Tai'an 271018 P.R.China

Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. Functional analysis showed that MdMYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR （qRT-PCR） assay indicated that the expression levels of cold-responsive genes including MdCBF2, MdCBF3, MdCBF4, and MdCBF5 were obviously enhanced in MdMYB73 transgenic calli, suggesting that MdMYB73 increased cold tolerance via C-repeat binding factor （CBF） cold response pathway. Finally, we found that soluble sugar, which provides an osmoticum for cells, was increased in MdMYB73 transgenic calli compared to that in the wild type control. These findings provide a new insights into how MdMYB73 is involved in cold stress response.

关键词： apple MdMYB73 cold stress CBF soluble sugar