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The Response of cockerel Semen Kinematic Parameters LIN, STR, WOB, ALH and BCF to Induced Oxidative Stress

Open Journal of Animal Sciences 2021年第2期11卷 292-303页

作者： Cyril Mpho Pilane Mohleko Helen Mapeka Agricultural Research Council Animal Production Campus Germplasm Conservation & Reproductive Biotechnologies Irene South Africa Department of Animal Production and Agricultural Economics Faculty of Science and Agriculture University of Limpopo Sovenga South Africa

Computer-assisted sperm class analyser (CASA) analysis of avian semen following cryopreservation indicates that their semen motility and viability parameters become compromised, due in part to oxidative stress. To mimic these observations we have treated cockerel semen with an oxidative stress inducing agent, namely hydrogen peroxide (H2O2) and monitored the motility, kinematic and viability parameters over time. Briefly, five healthy and fertile South African Venda cockerels were selected and their semen was collected using the abdominal massage technique. The semen was then treated with H2O2 at 0 µM, 5 µM, 50 µM and 200 µM concentrations for 0, 3, 16 and 24 hrs. The semen motility, kinematic and viability parameters were then determined using the CASA system while the viability was determined using the SYBR-14/PI staining. The Pearson’s correlation coefficient was determined to test the relationships between the levels of induced oxidative stress, period of exposure to oxidative stress inducing agent and the motility plus kinematic parameters. Our data revealed that in raw cockerel semen, there was high and positive correlations between total motility (TM), progressive motility (PM), rapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and average path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH and BCF had low or negative correlations with them. Furthermore, TM, PM, RAP, VCL and VSL remained highly and positively correlated with the induced oxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) remained negatively correlated with the induced oxidative stress, after 3 hrs. After 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated with the induced oxidative stress while LIN, STR, WOB and BCF became positively correlated with the induced oxidative

关键词： cockerel Oxidative Stress Kinematic Parameters Semen

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Comparative Growth Performance, Carcass Characteristics and Meat Quality of Local Horro and Exotic cockerels of Tropical Origin Fed Growers Diet

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Open Journal of Animal Sciences 2021年第1期11卷 62-83页

作者： Abrar Biazen Urge Mengistu Ameha Negassi Aseffa Getenet Abegaz Solomon Dessie Tadelle School of Animal and Rangeland Sciences Haramaya University Haramaya Ethiopia Land O’Lakes Addis Ababa Ethiopia Ethiopian Institute of Agricultural Research Addis Ababa Ethiopia International Livestock Research Institute Addis Ababa Ethiopia

Growth performance, carcass characteristics and meat quality of male chickens were evaluated using eight-week-old 360 cockerels from three exotic (Koekoek, Kuroiler and Sasso-R) and one Ethiopian indigenous (Horro) genotypes. cockerels in each breed (90) were randomly allocated into three replications of 30 birds each and housed in deep litter pens for the experiment that lasted for 8 to 16 weeks. The experiment was arranged in a Completely Randomized Design. Seven birds per breed were slaughtered at the end to determine carcass yield, chemical composition and quality attributes of meat. The Kuroiler breed has higher body weight, body weight change (1621.8 g), average daily gain (28.9 g/day), daily feed consumption (127.4 g/day) and better feed conversion ratio (4.40) than other breeds. The Kuroiler breed has heavier slaughter weight (2716.3 g), dressed carcass weight (2201.0 g), eviscerated carcass weight (1905.1 g), breast weight (537.7 g), thigh weight (303.1 g) and drumstick weight (274.8 g) than other breeds. Total edible offal was higher for

关键词： Breed Carcass Characteristics Chicken cockerel Growth Meat Quality

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Haematological and Serum Biochemistry Profile of cockerels Experimentally Infected with <i>Salmonella enterica</i>Serovar Zega

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Open Journal of Veterinary Medicine 2019年第12期9卷 171-184页

作者： Paulinus Ekenedilichukwu Emennaa Didacus Chukwuemeka Eze Foinkfu Kennedy Chah John Osita Arinze Okoye John Ikechukwu Ihejioha Milton Nancy Sati Ifeanyi Onyema Christian Okorie-Kanu Asabe Adamu Dzikwi-Emennaa Uchendu Chidiebere Kayode Olorufemi Akuchi Chidiabi Nwamo Jacintha Ngozi Omeke Israel Joshua Barde National Veterinary Research Institute Vom Nigeria Faculty of Veterinary Medicine University of Nigeria Nsukka Nigeria Faculty of Veterinary Medicine University of Jos Jos Nigeria

Salmonellosis is a serious medical and veterinary problem worldwide and causes great concern in the food and livestock industries, especially the poultry industry which occupies a prominent position in the provision of animal protein and accounts for about 25% of local meat production in Nigeria particularly and is identified as a disease of major economic importance causing low performance in poultry production. The study was carried out at the experimental animal farm, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria. One hundred (100) five-week old chickens obtained from the Poultry division of National Veterinary Research Institute, Vom, were used for the experiment. The birds were randomly assigned to 4 groups of 25 birds per group (A, B, C, D). Each bird in all the groups received 0.5 ml of PBS containing 1 × 108 cfu/ml of Salmonella enterica serovar Zega as follows: Group A was infected with Salmonella Zega intra-nasally (IN). Group B was infected with Salmonella Zega intra-peritonealy (IP). Group C was infected with Salmonella Zega orally (OR). Group D was the Uninfected control (CT). There was a significant change (p 0.05) in the mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) in the infected groups compared across the different days post infection. There was significant increase (p Salmonella Pullorum and Salmonella Gallinarum that are known to cause pathology in birds Salmonella Zega which is none host specific for birds can also cause pathology in them. This is the first report in the study area to the best of our knowledge.

关键词： Hematological Serum Biochemistry cockerel Salmonella Zega

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