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Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma

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Journal of Pharmaceutical Analysis 2023年第2期13卷 216-222页

作者： Wei Zhou Martyna N.Wieczorek Runshan Will Jiang Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooONN2L 3G1Canada Faculty of Food Science and Nutrition Poznan University of Life SciencesPoznanPoland

The direct coupling of solid-phase microextraction(SPME)to mass spectrometry(MS)(SPME-MS)has proven to be an effective method for the fast screening and quantitative analysis of compounds in complex matrices such as blood and *** recent years,our lab has developed three novel SPME-MS techniques:SPME-microfluidic open interface-MS(SPME-MOI-MS),coated blade spray-MS(CBS-MS),and SPME-probe electrospray ionization-MS(SPME-PESI-MS).The fast and high-throughput nature of these SPME-MS technologies makes them attractive options for point-of-care analysis and anti-doping ***,all these three techniques utilize different SPME geometries and were tested with different MS *** of comparative data makes it difficult to determine which of these methodologies is the best option for any given *** work fills this gap by making a comprehensive comparison of these three technologies with different SPME devices including SPME fibers,CBS blades,and SPME-PESI probes and SPME-liquid chromatography-MS(SPME-LC-MS)for the analysis of drugs of abuse using the same MS ***,for the first time,we developed different desorption chambers for MOI-MS for coupling with SPME fibers,CBS blades,and SPME-PESI probes,thus illustrating the universality of this *** total,eight analytical methods were developed,with the experimental data showing that all the SPME-based methods provided good analytical performance with R^(2)of linearities larger than 0.9925,accuracies between 81%and 118%,and good precision with an RSD%≤13%.

关键词： Solid-phase microextraction Mass spectrometry Microfluidic open interface coated blade spray Probe electrospray ionization Drug of abuse

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Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches

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Journal of Pharmaceutical Analysis 2022年第1期12卷 186-191页

作者： Karol Jaroch Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooN2L 3G1Canada Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Toruń Poland85090BydgoszczPoland

The solid-phase microextraction technique quantifies analytes without considerably affecting the sample ***,a proof-of-concept study was conducted to demonstrate the use of coated probe electrospray ionization(coated-PESI)and coated blade spray(CBS)as ambient mass spectrometry approaches for monitoring drug *** ability of these methods was investigated for monitoring the dephosphorylation of a prodrug,combretastatin A4 phosphate(CA4P),into its active form,combretastatin A4(CA4),in a cell culture medium supplemented with fetal bovine *** CBS spot analysis was modified to achieve the same extraction efficiency as protein precipitation and obtained results in 7 *** coated-PESI performs extraction without consuming any samples,it is the preferred technique in the case of a limited sample *** coated-PESI only extracts small quantities of analytes,it uses the desorption solvent volume of 5-10 pL,resulting in high sensitivity,thus allowing the detection of compounds after only 1 min of *** biotransformation of CA4P into CA4 via phosphatases occurs within the simple matrix,and the proposed sample preparation techniques are suitable for monitoring the biotransformation.

关键词： Solid-phase microextraction coated probe electrospray ionization coated blade spray Combretastatin Biotransformation Prodrug activation

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