检索结果-南通市图书馆

Transcription factor EHF interacting with coactivator AJUBA aggravates malignancy and acts as a therapeutic target for gastroesophageal adenocarcinoma

引用

Acta Pharmaceutica Sinica B 2024年第5期14卷 2119-2136页

作者： Li Peng Yanyi Jiang Hengxing Chen Yongqiang Wang Qiusheng Lan Shuiqin Chen Zhanwang Huang Jingyuan Zhang Duanqing Tian Yuntan Qiu Diankui Cai Jiangyun Peng Daning Lu Xiaoqing Yuan Xianzhu Yang Dong Yin Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong-Hong Kong Joint Laboratory for RNA MedicineSun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Medical Research Center Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Hefei Institutes of Physical Science Chinese Academy of SciencesHefei 230031China Department of Gastrointestinal Surgery Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Department of Hepatobiliary Surgery Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou 511442China

Transcriptional dysregulation of genes is a hallmark of tumors and can serve as targets for cancer drug ***,it is extremely challenging to develop small-molecule inhibitors to target abnormally expressed transcription factors(TFs)except for the nuclear receptor family of *** is known about the interaction between TFs and transcription cofactors in gastroesophageal adenocarcinoma(GEA)or the therapeutic effects of targeting TF and transcription cofactor *** this study,we found that ETS homologous factor(EHF)expression is promoted by a core transcriptional regulatory circuitry(CRC),specifically ELF3-KLF5-GATA6,and interference with its expression suppressed the malignant biological behavior of GEA ***,we identified Ajuba LIM protein(AJUBA)as a new coactivator of EHF that cooperatively orchestrates transcriptional network activity in ***,we identified KRAS signaling as a common pathway downstream of EHF and ***,dual targeting of EHF and AJUBA by lipid nanoparticles cooperatively attenuated the malignant biological behaviors of GEA in vitro and in *** conclusion,EHF is upregulated by the CRC and promotes GEA malignancy by interacting with AJUBA through the KRAS *** of both EHF and its coactivator AJUBA through lipid nanoparticles is a novel potential therapeutic strategy.

关键词： EHF AJUBA KRAS pathway Enhancer Core transcriptional regulatory circuitry Gastroesophageal adenocarcinoma Gastric adenocarcinoma Esophageal adenocarcinoma Transcription factor coactivator Lipid nanoparticles

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CREB-binding proteins (CBP) as a transcriptional coactivator of GATA-2

引用

Science China(Life Sciences) 2008年第3期51卷 191-198页

作者： TOMOMI Takahashi TORU Nakano Department of Pathology Medical School and Graduate School of Frontier Biosciences Osaka University Yamada-oka 2-2 Suita Osaka 565-0871 Japan

The GATA family consists of six members, GATA 1-6. In this study, we focused on GATA-2, which is expressed predominantly in hematopoietic progenitor cells and plays the key role in keeping these cells in the undifferentiated status. CREB-binding proteins (CBP) are essential transcriptional coacti- vators for a large number of regulated DNA-binding transcription factors, including GATA-1. But there have been no reports on whether CBP is still a co-activator of GATA-2. Here, we used the immunopre- cipitation and pull-down experiments to show that the GATA-2 and CBP were physically binding to- gether, and clarified the binding sites CH1, CH3 , CH452 and CT1430 in CBP and N-finger, C-finger and N-C-finger in GATA-2. Luciferase assay results in our experiment indicated that CBP could increase GATA-2 transcriptional activity in the dose-dependent manner. GATA-1 is mainly expressed in differen- tiated hematopoietic cells, but still has overlap expression with GATA-2. CBP is a coactivator of GATA-2 and GATA-1. The investigation on the mechanism that could decide whether CBP binds to GATA-2 to keep hematopoietic cells in the progenitor status or to GATA-1 to start differentiation will be a very in- teresting and very meaningful project in the near future.

关键词： CBP TATA-2 coactivator hematopoietic cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibitory roles of protein kinase B and peroxisome proliferator-activated receptor gamma coactivator on hepatic HMG-CoA reductase promoter activity

引用

Advances in Bioscience and Biotechnology 2013年第10期4卷 1-5页

作者： Gene C. Ness Jeffrey L. Edelman Department of Molecular Medicine Morsani College of Medicine University of South Florida Tampa USA

Since we had previously demonstrated that siRNAs to tristetraprolin (TTP) markedly inhibited insulin stimulation of hepatic HMG-CoA reductase (HMGR) transcription, we investigated the effects of transfecting rat liver with TTP constructs. We found that transfecting diabetic rats with TTP did not increase HMGR transcription but rather led to modest inhibition. We then investigated whether co-transfection with protein kinase B, hepatic form (AKT2), might lead to phosphorylation and result in activation of HMGR transcription. We found that this treatment resulted in near complete inhibition of transcription. Transfection with peroxisome proliferator-activated receptor g coactivator (PGC-1a) also inhibited HMGR transcription. These results show that although TTP is needed for activation of HMGR transcription, it cannot by itself activate this process. AKT2 and PGC-1a, which mediate the activation of gluconeogenic genes by insulin, exert the opposite effect on HMGR.

关键词： In Vivo Electroporation HMG-CoA Reductase Insulin Protein Kinase B Peroxisome Proliferator-Activated Receptor γ coactivator Tristetraprolin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gelsolin is a novel coactivator of ERa in Her-2/neu over-expressing breast cancer cells

Gelsolin is a novel coactivator of ERa in Her-2/neu over-exp...

引用

第九届西南三省一市生物化学与分子生物学学术交流会

作者：李渝萍 See Wai Kenneth Yau 周度金第三军医大学生物化学与分子生物学教研室

To better understand the mechanism of ERa-mediated regulation in Her-2/neu over-expressing breast cancer cells,we have searched for cytosolic protein(s) that interacts with ERα.Through immunoprecipitation with ERα a... 详细信息

关键词： gelsolin coactivator ERα Her-2/neu breast cancer cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of Mitochondrial Dysfunction via AMPK/PGC-1α Signal Pathway on Pathogenic Mechanism of Diabetic Peripheral Neuropathy and the Protective Effects of Chinese Medicine

引用

Chinese Journal of Integrative Medicine 2019年第5期25卷 386-394页

作者： ZHANG Qian LIANG Xiao-chun Department of Traditional Chinese Medicine Translational Medicine CenterPeking Union Medical College HospitalChinese Academy of Medical SciencesBeijing(100730)China

Diabetic peripheral neuropathy(DPN) is a progressive neurodegenerative disease of peripheral nervous system with high energy requirement. The adenosine monophosphate-activated protein kinase(AMPK)/peroxisome proliferator-activated receptor-γ coactivator 1α(PGC-1α) axis plays a key role in regulating mitochondrial energy metabolism. Increasing preclinical evidences have shown that inhibition of AMPK/PGC-1α pathway leading to mitochondrial dysfunction in neurons or Schwann cells contributes to neuron apoptosis, distal axonopathy and nerve demyelination in DPN. Some Chinese medicine formulae or extracts from herbs may have potential neuroprotective effects on DPN via activating AMPK/PGC-1α pathway and improving mitochondrial function.

关键词： monophosphate-activated protein kinase peroxisome proliferator-activated receptor-γ coactivator 1α sirtuins diabetic peripheral neuropathy Chinese medicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Estrogen Receptor α(ERα) Target Gene LRP16 Interacts with ERα and Enhances Receptor's Transcriptional Activity

引用

Chinese Journal of Cancer Research 2007年第4期19卷 233-237页

作者：韩为东赵亚力吴志强孟元光臧丽母义明 Department of Molecular Biology Institute of Basic Medicine Department of Obstetrics and Gynecology Department of Endocrinology PLA General Hospital

Objective：It has been shown that LRP16 is an estrogen-induced gene through its receptor α（ERα）. Although there is evidence demonstrating that inhibition of LRP16 gene expression in MCF-7 human breast cancer cells partially attenuates its estrogen-responsiveness, the underlying molecular mechanism is still unclear. Here, the effect of LRP16 expression on the ERα signaling transduction was investigated. Methods： Cotransfection assays were used to measure the effect of LRP16 on ERα-mediated transcriptional activity. GST-pulldown and immunoprecipitation （ColP） assays were employed to investigate the physical interaction of LRP16 and ERα. The mammalian two-hybrid method was used to map the functional interaction region. Results： the results of cotransfection assays demonstrated that the transcriptional activities of ERα were enhanced in α LRP16 dose-dependent manner in MCF-7 in the presence of estrogen, however, it was abolished in the absence of E2 in MCF-7 cells. The physical interaction of LRP16 and ERα proteins was confirmed by GST-pulldown in vitro and ColP in vivo assays, which was enhanced by E2 but not dependent on its presence. Furthermore, the results of the mammalian two-hybrid assays indicated that the binding region of ERα to LRP16 located at the A/B AF-1 functional domain and E2 stimulated the binding of LRP16 to the full-length ERα molecule but not to the A/B region alone. Conclusion： These results support a role for estrogenically regulated LRP16 as an ERα coactivator, providing a positive feedback regulatory loop for ERα signal transduction. Based on this function of LRP16, we propose that ERα-positive breast cancer patients with high expression of LRP16 might benefit from targeting LRP16 therapy.

关键词： Estrogen receptorα LRP16 Interaction coactivator

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gelsolin can up-regulate the trans-activity of ERa in Her-2/neu over-expressing breast cancer cells

Gelsolin can up-regulate the trans-activity of ERa in Her-2/...

引用

中国生物化学与分子生物学会第十届会员代表大会暨全国学术...

作者： Yuping Li See Wai Kenneth Yau Jingjing Ye Ki Lui Dujin Zhou Shiuan Chen Third Military Medical University Beckman Research Institute o f City of Hope CA 91010 Beckman Research Institute of City of Hope CA 91010

To better understand the mechanism of ERa-mediated regulation in Her-2/neu overexpressing breast cancer cells, we have searched for cytosolic protein(s) that interacts with ERα.Through immunoprecipitation with ERαantibody and mass spectrometric analysis,we identified the protein gelsolin(GSN) that only interacted with ERa in the estradiol (E2)- treated,ERα-transfected and Her-2/neu over-expressing breast cancer cell line *** is an actin severing protein for cytoskeleton reorganization,cell morphology and *** protein was found to serve as a coactivator when SK-BR-3 cells were transfected with pCI-ERαand pCI-GSN and treated with *** gelsolin-mediated inductive effect was also detected in two additional Her-2/

关键词： gelsolin coactivator ERa Her-2/neu breast cancer cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification and Characterization of PNRC splicing variants

Identification and Characterization of PNRC splicing variant...

引用

第九届西南三省一市生物化学与分子生物学学术交流会

作者：周度金第三军医大学生物化学与分子生物学教研室

Nuclear receptor(NR)dependent transcriptional action requires recruitment of diverse factors characterized as ***(Proline-rich nuclear receptor coregulatory protein)is a member of coregulators that are capable of pote... 详细信息

关键词： PNRC alternative splicing isoform nuclear receptor coactivator estrogen receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：