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Conformation Disturbance of copc by α-Naphthylamine

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Chemical Research in Chinese Universities 2009年第3期25卷 269-272页

作者： LI Hui-qing ZHENG Xiao-yan ZHAO Ya-qin YANG Bin-sheng Institute of Molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Shanxi University Taiyuan 030006 P. R. China

It is known that urea and guanidine hydrochloride（GuHC1） induce conformational change of proteins in a certain range of molar ratios. In our research, a-naphthylamine（NA） above 10^-4 mol/L at pH 7.0 was discovered to perturb the conformation of copc, a copper resistant protein with a Greek fl-barrel motif; this was reflected by the greater fluorescence quenching and red-shifted emission peak of copc. The conformation change of copc was also verified in acrylamide collision experiment by comparing quenching levels of copc in the presence or the abscence of NA. Comparison of emission change of copc-Cu^2＋ with that of apo-copc showed a consistent result with their denaturation in GuHCl. And decreasing fluorescence polarization of copc with increasing NA concentration is consistent with a more extend conformation of copc. Interaction mechanism was also explored.

关键词： α-Naphthylamine(NA) copc Conformational change

The role of cupric in maintaining the structure of copc

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Chinese Science Bulletin 2007年第6期52卷 743-747页

作者： ZHENG XiaoYan PANG ErGuo LI HuiQin ZHAO YaQin YANG BinShengt Institute of Molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education ShanxiUniversity Taiyuan 030006 China

The copc protein from pseudomonas syringae pathovar tomato is expressed as one of four proteins encoded by the operon CopABCD that is responsible for copper resistance. And there are one trypto-phan (83), one tyrosine (79), and three phenylalanines (35, 43, 99) in apocopc. The fluorescence peak of apocopc is located near 320 nm, and the peak shifts toward 353 nm in the presence of 10 mmol·L^(-1) urea with excitation at 280 nm. Using urea as a chemical agent, the conformational stabilities of apocopc and Cu_N^(2+) -copc were monitored by fluorescence spectrum in 20 mmol·L^(-1) phosphate buffer and 100 mmol·L^(-1) sodium chloride at pH 6.0. The free energy of stabilization for apocopc and Cu_N^(2+)-copc is 16.29±0.65 kJ·mol^(-1) and 26.26±0.35 kJ·mol^(-1), respectively. The distance between the tryptophan residue and the Cu^(2+) in Cu_N^(2+) -copc has been studied by observing Frster type nonradia-tive energy transfer. And it is calculated to be 11.6 .

关键词：二价铜 copc 蛋白质结构构象稳定性作用尿素变性

copc在八面沸石笼中的原位合成及性能研究

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燃料化学学报 2004年第1期32卷 83-86页

作者：宋芬宋明纲范彬彬李瑞丰太原理工大学精细化工研究所山西太原030024

采用苯酐-尿素路线,对copc金属配合物在八面沸石超笼中原位合成进行了考察,并采用多种物化手段和催化反应对所制备的copcY进行了详细的表征。结果表明,该制备路线是一简便有效的合成路线,采用该路线可在八面沸石超笼中原位合成copc金属... 详细信息

采用苯酐-尿素路线,对copc金属配合物在八面沸石超笼中原位合成进行了考察,并采用多种物化手段和催化反应对所制备的copcY进行了详细的表征。结果表明,该制备路线是一简便有效的合成路线,采用该路线可在八面沸石超笼中原位合成copc金属配合物并将之固定于八面沸石中。在该制备方法中合成温度、离子交换所采用的盐类型及催化剂对copc金属配合物在八面沸石超笼中的合成及所制备的copcY在环己烷氧化反应中的催化性能有很大的影响,其中180℃为最佳合成温度,氯化钴较适合作为交换的盐类。在八面沸石超笼中原位合成的copc金属配合物在温和的反应条件下对环己烷氧化具有良好催化活性,转化数TON最高可达5000以上。

关键词：酸酐路线 copc 八面沸石环己烷氧化

copc-Catalyzed Selective Radical Arylation of Anilines with Arylhydrazines for Synthesis of 2-Aminobiaryls

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CoPc-Catalyzed Selective Radical Arylation of Anilines with ...

Mechanism of copper（Ⅱ） transfer from copc to Mcopc

中国化学会全国第十一届有机合成化学学术研讨会

作者： Tao Jiang Sheng-Yan Chen Run-Sheng Zeng Shao-Fang Zhou Jian-Ping Zou Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry and Chemical EngineeringSoochow University Key Laboratory of Synthetic Chemistry of Natural Substances Shanghai Institute of Organic ChemistryChinese Academy of Science

Biaryls have attracted considerable attention within the chemical sciences because of their applications in organic synthesis,pharmaceuticals and materials. A well-known method for synthesis of biaryls is the Gomberg-Bachmann reaction(first reported in 1924) using aryldiazonium salts by homolytic aromatic substitution. Arylhydrazine is one of the radical precursors,it was effectively applied in organic synthesis in recent *** paper presents a novel copc-catalyzed regioselective radical arylation of anilines with arylhydrazines.

关键词： radical reaction arylhydrazine copc

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Mechanism of copper（Ⅱ） transfer from CopC to MCopC

Issue in Implementing Customer Operations Performance Center (copc)

中国化学会第28届学术年会

作者： Hui-Qing Li1,2,Bin-Sheng Yang2,* 1 Chemistry Department,Xinzhou Techaers''Universty,Xinzhou 034000 2 Institute of Molecular Science,Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan,Shanxi 030006

＜正＞copc functions by sequestrating and transferring copper with other Cop proteins in the oxidizing periplasmic *** kinetics of copper（II）transfer from copc to Mcopc,stimulation of Cop protein,in comparisons with to

关键词： copc Mechanism

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Journal of Software Engineering and Applications 2013年第8期6卷 431-433页

作者： Yingyu Xu Xinyuan Xi Yuki Todo China Soft International Technology Service Ltd. Shanghai China Department of Electrical and Computer Engineering Kanazawa University Kanazawa Japan LeLe Mother’s Maternal Store Ltd. Shanghai China

Customer Operations Performance Center Inc (copc) is the world’s leading authority on Contact Center Operations Management. The copc 2000 Standard is a comprehensive operating model for Contact Centre Operations. It is a world-wide benchmark and certification for contact centers. As well as an overall performance management system, copc is designed to reduce costs, increase revenue, and improve service, quality and customer satisfaction. However, many copc implementation projects failed. In this study, we first review the process of implementing copc. Then, we identify major barriers in implementing copc. Organizations should take proper measures in overcoming these barriers to ensure successful implementation of copc.

关键词： copc IT Management

Spectra study on the ternary complex of Cu（Ⅱ）, doxorubicin and copc

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Spectra study on the ternary complex of Cu（Ⅱ）, doxorubici...

Hemin和copc相互作用的光谱和分子模拟研究

中国化学会第十四届全国生物无机化学学术会议

作者： Yunxi Song Bingsheng Yang Insitute of Molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Shanxi University

copc, which is a copper binding protein, can bind both Cu（Ⅱ） and Cu（Ⅰ） [1,2,3]. Doxorubicin is an anthracycline antibiotics [4]. The interaction between doxorubicin and copc may affect the role of copc as redox switch. Fluorescence and UV spectra showed that Cu（Ⅱ）, doxorubicin, and copc can form a ternary complex. The formation of copc-Cu（Ⅱ）-doxorubicin decreases greatly reduction rate of Cu（Ⅱ） by ascorbate, the binding of doxorubicin affects the action of copc as redox swich. The conclusions may provide meaningful information for illustrating the copper regulation mechanism.

关键词： Doxorubicin copc Cu(Ⅱ)

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化学研究与应用 2021年第3期33卷 493-499页

作者：宋珍张智超杨斌盛太原师范学院化学系山西晋中030619 山西大学分子科学研究所化学生物学与分子工程教育部重点实验室山西太原030006

本文通过光谱技术以及分子模拟手段研究了copc与hemin的相互作用。紫外可见光谱表明hemin中Fe^(3+)可能与copc中的组氨酸(His1或His91)形成配位。此外,荧光光谱表明copc与hemin的主要弱作用力为疏水作用力。圆二色谱、化学变性、荧光寿... 详细信息

本文通过光谱技术以及分子模拟手段研究了copc与hemin的相互作用。紫外可见光谱表明hemin中Fe^(3+)可能与copc中的组氨酸(His1或His91)形成配位。此外,荧光光谱表明copc与hemin的主要弱作用力为疏水作用力。圆二色谱、化学变性、荧光寿命以及同步荧光实验表明hemin与copc的配位使得copc的稳定性降低,并且色氨酸周围环境的疏水性减弱。紫外可见及荧光光谱表明,两者结合常数在10^(6)左右,结合比为1,hemin与copc中唯一的色氨酸(Trp83)的距离为1.06 nm。最后通过分子对接模拟了copc与hemin的相互作用,结果表明hemin中Fe^(3+)可能与copc中的组氨酸(His1)形成与卟啉环平面垂直的轴向配位,并且与Trp83距离为0.96 nm,与实验结果一致。

关键词： copc hemin 荧光

copc催化的芳基肼与芳胺的选择性反应研究

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CoPc催化的芳基肼与芳胺的选择性反应研究

作者：蒋涛苏州大学

学位级别：硕士

联苯衍生物和N,N-二芳基胺衍生物是重要的化工产品和中间体，它们在医药、染料、农药和材料中具有广泛的用途。自由基合成法是一类有效的合成方法，其中过渡金属催化的自由基反应占有重要的位置。本文首次报道了copc催化的芳基肼与芳胺... 详细信息

联苯衍生物和N,N-二芳基胺衍生物是重要的化工产品和中间体，它们在医药、染料、农药和材料中具有广泛的用途。自由基合成法是一类有效的合成方法，其中过渡金属催化的自由基反应占有重要的位置。本文首次报道了copc催化的芳基肼与芳胺的反应，选择性地得到了联苯衍生物和N,N-二芳基胺衍生物。本论文分为两部分：第一部分研究了copc催化的芳基肼与芳胺的反应，选择性地得到了2-氨基联苯衍生物;第二部分研究了copc/Cu(OAc)2共催化的芳基肼和芳胺的反应，选择性地得到了N,N-二芳基胺衍生物。产物的结构经核磁共振谱、质谱等得到了证实，在此基础上提出了反应的机理。

关键词：芳基肼自由基反应 copc