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Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts co-cultured with Osteoblasts

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Journal of Biomedical Science and Engineering 2017年第1期10卷 21-30页

作者： Jian Chen Hui Huang Jianquan He Zhang Xin Department of Rehabilitation Zhongshan Hospital of Xiamen University Xiamen University Xiamen China

The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.

关键词： PEMFs co-cultured RANK mRNA NFATc1 mRNA

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The Skin Whitening Effect of co-cultured conditioned Medium: Involvement of Synergy between Stem Cells and Immune Cells

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Journal of cosmetics, Dermatological Sciences and Applications 2021年第4期11卷 342-355页

作者： Tae-Kyung Ko Hye-Mi Hong Dong-Soo Kim Da-Young Yoo Sung Hwan Hwang Jung-Hwa Kang Research & Development Smart Cell Lab. Co. Ltd. B2 International St. Mary’s Hospital Incheon Korea Research & Development Immunisbio Co. Ltd. B2 International St. Mary’s Hospital Incheon Korea

Many researchers have described that mesenchymal stem cells conditioned medium and immune cells conditioned medium have a clear whitening effect when they are used as cosmetic ingredients. In this study, we confirmed the whitening efficacy of various concentrations of immune cells and stem cell conditioned media. The author tried to study a conditioned medium that has a strong whitening effect even with a composition of less than 20% (the most used concentration in cosmetics). Because of the fact that the conditioned medium contains various cytokines and growth factors secreted by stem cells or immune cells, it is known to have effects such as wound healing, antioxidant, and whitening effect. Recently, stem cells have been used not only in the development of cosmetic raw materials but also in skincare procedures, and there are reports being released of cosmetics using immune cells conditioned medium. The concentration-dependent whitening effect equivalently increased as the concentration of the mono-cultured conditioned medium was obtained through the stem cells or immune cells culture. In the case of co-culture, whitening results are like the effect of positive control such as arbutin in the medium carrying only 10% of the co-cultured conditioned medium. It is possible that enhanced whitening efficiency in co-cultured conditioned medium leads to a major innovation in the global cosmetic market.

关键词： Stem Cell Immune Cell Natural Killer Cell (NK Cell) conditioned Medium co-cultured Skin Whitening

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Preclinical stem cell therapy in Chagas Disease: Perspectives for future research

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World Journal of Transplantation 2013年第4期3卷 119-126页

作者： Katherine Athayde Teixeira de Carvalho Eltyeb Abdelwahid Reginaldo Justino Ferreira Ana Carolina Irioda Luiz Cesar Guarita-Souza Cell Therapy and Biotechnology in Regenerative Medicine Research Group Pequeno Príncipe FacultyPelé Pequeno Príncipe InstituteCuritiba 80250-200ParanáBrazil Bioprocess Engineering and Biotechnology Department Federal University of ParanáCuritiba 81531-970ParanáBrazil CBRC Massachusetts General Hospital/Harvard Medical SchoolCharlestownMa 02129United States Federal University of Technology Curitiba 80230-901ParanáBrazil Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Parana Curitiba 80215-901ParanáBrazil

Chagas cardiomyopathy still remains a challenging problem that is responsible for high morbidity and mortality in Central and Latin America. Chagas disease disrupts blood microcirculation via various autoimmune mechanisms, causing loss of cardiomyocytes and severe impairment of heart function. Different cell types and delivery approaches in Chagas Disease have been studied in both preclinical models and clinical trials. The main objective of this article is to clarify the reasons why the benefits that have been seen with cell therapy in preclinical models fail to translate to the clinical setting. This can be explained by crucial differences between the cellular types and pathophysiological mechanisms of the disease, as well as the differences between human patients and animal models. We discuss examples that demonstrate how the results from preclinical trials might have overestimated the efficacy of myocardial regeneration therapies. Future research should focus, not only on studying the best cell type to use but, very importantly, understanding the levels of safety and cellular interaction that can elicit efficient therapeutic effects in human tissue. Addressing the challenges associated with future research may ensure the success of stem cell therapy in improving preclinical models and the treatment of Chagas disease.

关键词： Chagas Disease preclinical stem cell therapy co-cultured translation pathophysiologie myoblasts

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