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Disulfide bond reduction corresponds to dimerization and hydrophobi-city changes of clostridium botulinum type A neurotoxin

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Acta Pharmacologica Sinica 2006年第9期27卷 1238-1246页

作者： Jiunn-jye WEY~1 Shiao-shek TANG~1 Tzong-yuan WU~(2,3)1 Institute of Preventive Medicine,National Defense Center,Taipei,Taiwan 115,China 2 Department of Bioscience Technology,Chung Yuan Christian University,Chungli,Taiwan 320,China Institute of Preventive Medicine National Defense Center Taipei China Department of Bioscience Technology Chung Yuan Christian University Chungli China

Aim:To determine the structure factors that mediate the intoxication process ofbotulinum neurotoxin type A(BoNT/A).Methods:Triton X-114 phase separationexperiments and 1-anilino-8-naphthalene sulfonate binding assay were used tostudy the structural factor that corresponds to the hydrophobicity change ofBoNT/*** addition,sucrose density gradient centrifugation and a chemicalcrosslinking study were employed to determine the quaternary structure of BoNT/***:Our results demonstrated that in other than acidic conditions,thedisulfide reduction is the structural factor that corresponds to the hydrophobicitychange of BoNT/*** quaternary structure of BoNT/A exists as a dimmer inacidic solution(pH 4.5),although the monomeric structure of BoNT/A was re-ported based on X-ray ***:Disulfide bond reduction iscritical for BoNT/A’s channel formation and ability to cross endosome *** result implies that compounds that block this disulfide bond reduction mayserve as potential therapeutic agents for botulism.

关键词： clostridium botulinum botulinum neurotoxin A hydrophobicity phase partition

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Expression of Hc fragment from clostridium botulinum neurotoxin serotype B in Escherichia coli and its evaluation as vaccine candidate

Expression of Hc fragment from Clostridium botulinum neuroto...

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第九届中国生物毒素学术研讨会

作者： Yan-Li GAO,Shan GAO,Lin KANG,Chong NIE,Jing-Lin WANG Laboratory of Toxinology,State key Laboratory of Pathogen and Biosecurity,The institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,No.20 Dongda Street,Fengtai district, Beijing 100071,P.R China

＜正＞A good immunogen was developed as an effective recombinant vaccine candidate that protected mice against botulinum neurotoxin serotype B（BoNT/B） *** Hc fragment of clostridium botulinum neurotoxin type B（rBoNT/B-Hc） was cloned into a bacterial expression vector pQE-30,and the resulting vector was successfully expressed in Escherichia coli M15 *** purified rBoNT/B-Hc protein was used to vaccinate mice and evaluate their survival against challenge with native BoNT/*** mice,that received three subcutaneous injections at rBoNT/B-Hc immunogen dosages ranging from 0.25 to 6.25μg mixed with Freund adjuvant,were completely protected against an intraperitoneal administration of 10,000 50%lethal dosages（LD50） of BoNT/B.A dose-response was observed in both the ELISA antibody titers and protective efficacy with increasing dosage of *** work presented here demonstrates that the purified rBoNT/B-Hc was a highly effective immunogen and able to protect against a high dose of neurotoxin challenge.

关键词： clostridium botulinum botulinum neurotoxin Hc fragment vaccine

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An Overview of Type E Botulism in China

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Biomedical and Environmental Sciences 2008年第4期21卷 353-356页

作者： SI-WU FU CHEN-IUAI WANG Department of Microbiology Medical College Northwest University for Nationalities Lanzhou Institute of Biological Products Lanzhou 730030 Gansu China

The geographical distribution of C. botulinum type E and its associated disease, type E botulism in China, is different from that in other areas of the world. Cases of type E botulism generally arise in costal regions. In China, however, type E botuhsm is found primarily in the Qinghai-Tibet plateau of northwest China far from the ocean, at an altitude of approximately 4-5 kin. The foods most commonly associated with the disease are fermented grain and beans as well as raw meat A suspected outbreak of type E botulism poisoning in the central costal region of China in the 1990s prompted the collection and analysis of samples of mud, sand, and fish from the region. The toxin produced by type E botulinum was found in these samples. Surprisingly, though, upon further analysis, the strain isolated from the samples was identified not as type E C. botulinum, but as the neurotoxigenic bacterium Clostridiurn butyricurn.

关键词： clostridium botulinum Botulism Geographical distribution

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Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China

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Biomedical and Environmental Sciences 2017年第6期30卷 460-464页

作者： DONG Yin Ping WANG Wei JIANG Tao XU Jin HAN Chun Hui YAN Shao Fei Séamus Fanning LI Ying MA Xiao Chen ZHANG Di ZHAO Yao ZENG Biao LI Feng Qin Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment (CFSA) Beijing 100021 China School of Public Health Physiotherapy & Sports Science University College Dublin Dublin D04 N2E5 Ireland Children's Hospital Affiliated to Capital Institute of Pediatrics Beijing 100020 China Beijing Centers for Disease Control and Prevention Beijing 100013 China

Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula （PIF） presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins （BoNTs） and monovalent BoNT type B antitoxin had protective effects. clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis （PFGE） revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated bv type B C. botulinum spores.

关键词： clostridium botulinum Infantbotulism Powdered infant formula China

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