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clonal spread of Escherichia coli O101:H9-ST10 and O101:H9-ST167 strains carrying fosA3 and blaCTX-M-14 among diarrheal calves in a Chinese farm,with Australian Chroicocephalus as the possible origin of E.coli O101:H9-ST10

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Zoological Research 2021年第4期42卷 461-468页

作者： Wan-Yun He Xing-Xing Zhang Guo-Long Gao Ming-Yi Gao Fa-Gang Zhong Lu-Chao Lv Zhong-Peng Cai Xing-Feng Si Jun Yang Jian-Hua Liu College of Veterinary Medicine South China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China State Key Laboratory for Sheep Genetic Improvement and Healthy Production Institute of Animal Husbandry and VeterinaryXinjiang Academy of Agricultural and Reclamation ScienceShiheziXinjiang 832000China Zhejiang Tiantong Forest Ecosystem National Observation and Research Station School of Ecological and Environmental SciencesEast China Normal UniversityShanghai 200241China

During a 2018 antimicrobial resistance surveillance of Escherichia coli isolates from diarrheal calves in Xinjiang Province,China,an unexpectedly high prevalence(48.5%)of fosfomycin resistance was observed.This study aimed to reveal the determinants of fosfomycin resistance and the underlying transmission mechanism.Polymerase chain reaction(PCR)screening showed that all fosfomycin-resistant E.coli carried the fosA3 gene.Pulsed-field gel electrophoresis(PFGE)and southern blot hybridization revealed that the 16 fosA3-positive isolates belonged to four different PFGE patterns(i.e.,A,B,C,D).The fosA3 genes of 11 clonally related strains(pattern D)were located on the chromosome,while others were carried by plasmids.Whole-genome and long-read sequencing indicated that the pattern D strains were E.coli O101:H9-ST10,and the pattern C,B,and A strains were O101:H9-ST167,O8:H30-ST1431,and O101:H9 with unknown ST,respectively.Among the pattern C strains,the blaCTX-M-14 gene was co-localized with the fosA3 gene on the F18:A-:B1 plasmids.Interestingly,phylogenetic analysis based on core genome single nucleotide polymorphisms(cgSNPs)showed that the O101:H9-ST10 strains were closely related to a Australian-isolated Chroicocephalus-origin E.coli O101:H9-ST10 strain producing CTX-M-14 and FosA3,with a difference of only 11 SNPs.These results indicate possible international dissemination of the high-risk E.coli clone O101:H9-ST10 by migratory birds.

关键词： clonal spread Bovine fosA3 blaCTX-M-14 O101:H9-ST10 Chroicocephalus

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Epidemiology and genetic characterization of tet(X4)-positive Klebsiella pneumoniae and Klebsiella quasipneumoniae isolated from raw meat in Chengdu City, China

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Biosafety and Health 2024年第2期6卷 116-124页

作者： Weishuai Zhai Yiqing Wang Honghu Sun Bo Fu Qidi Zhang Congming Wu Jianzhong Shen Dejun Liu Yang Wang National Key Laboratory of Veterinary Public Health and Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Technology Innovation Center for Food Safety Surveillance and Detection Sanya InstituteChina Agricultural University Sanya 572025China Irradiation Preservation Key Laboratory of Sichuan ProvinceChengdu Institute of Food InspectionChengdu 611135China Colge of Veternary Medicine Qingdao Agricultural UniversityQingdao 266109China

The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to inves-tigate the epidemic features and transmission dynamics of tet(X4)-positive Klebsiella pneumoniae(K.pneumo-niae)through the analysis of 206 raw meats,including pork(n=182),beef(n=16),duck(n=5),and chicken(n=3).These samples were collected from schools,markets,and restaurants in Chengdu City,China.A total of 25 isolates were obtained from 13 administrative regions.All isolates exhibited resistance to tetracycline,tigecycline,ampicillin,chloramphenicol,and florfenicol.Over half of the isolates also demon-strated resistance to streptomycin(80%),sulfamethoxazole/trimethoprim(72%),ciprofloxacin(64%),and ampicillin/sulbactam(56%).Among these strains,14 distinct sequence types(STs)were identified,revealing evidence of inter-regional clonal spread,notably among 9 K.pneumoniae ST3393.Phylogenetic analysis revealed the presence of two K.pneumoniae ST5 closely resembling hypervirulent K.pneumoniae from Jiangsu.Importantly,12 isolates were capable of transferring tigecycline resistance to Escherichia coli J53.Further plasmid analysis showed that the tet(X4)-harboring plasmids in K.pneumoniae could be classified into four types,primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid(n=16)and IncFII plasmid(n=7),which significantly contributed to the cross-species dissemination of tet(X4).In summary,this study highlights the prevalence of MDR tet(X4)-positive K.pneumoniae in Chengdu,driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer.These findings emphasize the importance of contin-uous surveillance of tet(X4)-positive K.pneumoniae in raw meat and the implementation of effective measures to control their spread.

关键词： tet(X4) Tigecycline K.pneumoniae clonal spread Horizontal transfer

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