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Molecular recognition triggered aptazyme cascade for ultrasensitive detection of exosomes in clinical serum samples

Chinese Chemical Letters 2021年第5期32卷 1827-1830页

作者： Kemei Jiang Yanan Wu Juan Chen Mingqing Shi Hong-Min Meng Zhaohui Li College of Chemistry Green Catalysis CenterHenan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalyrical ApplicationsZhengzhou Key Laboratory of Functional Nanomaterial and Medical TheranosticZhengzhou UniversityZhengzhou 450001China

Exosomes have attracted widespread interest due to their inherent advantages in tumor diagnosis and treatment ***,it is still a big challenge for highly sensitive and specific detection of exosome in real complexed ***,a molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum *** this design,one target exosome could capture a large quantity of aptazymes for the first-step signal *** then the captured aptazyme was activated and recycled to release the fluorophore-labelled substrate strand for a cascaded signal ***,the activation of aptazyme only occurs whenithas bound with target exosome,ensuring a low *** experimental results show that the limit of detection(LOD)and the limit of quantification(LOQ)are 3.5×10^(3) particles/μL and 1.7×10^(4) particles/μL,respectively,which is comparable to the results of most existed fluorescence-based exosome ***,this assay possesses high specificity to distinguish exosomes derived from other cell ***,this fluorescence probe was utilized in cancer patient and healthy serum samples successfully,suggesting its great potential for clinical diagnosis and biological studies.

关键词： Aptazyme Exosomes Fluorescence probe Signal amplification clinical serum samples

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