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Survey and Detection of Disease and Pest Occurrence in citrus Nurseries

Plant diseases and pests 2024年第5期15卷 19-23页

作者： Liying GUO Qinghao SONG Ying HE Jiayi TANG Xiaoting ZHUANG Guochan HUANG Qianhua JI Yaping HU Institute of Pomology Zhaoqing UniversityZhaoqing 526361China College of Life Sciences Zhaoqing UniversityZhaoqing 526361China Sihui Science and Technology Demonstration Park Zhaoqing 526061China

[Objectives]The paper was to ascertain the prevalence of diseases and pests in a range of citrus nurseries situated in Guangdong Province and its neighboring provinces.[Methods]citrus diseases and pests were systematically investigated,and citrus leaf samples were randomly collected from 15 citrus nurseries across 8 regions in Guangdong Province and its neighboring *** polymerase chain reaction(qPCR)and reverse transcription polymerase chain reaction(RT-PCR)techniques were employed to detect diseases in the collected ***,root and substrate samples were obtained,and root-knot nematodes were isolated using the Baermann funnel method.[Results]The positive detection rate of citrus huanglongbing(HLB)was recorded at 3%,indicating an increase in attention towards this disease compared to ***,the positive detection rate for citrus bacterial canker disease(CBCD)was found to be 16.5%.It was observed that the majority of nurseries with positive samples employed open field rearing practices without the use of mesh chambers,and the primary source of scions was *** detection rate of citrus tristeza virus(CTV)was found to be the highest,with a positive detection rate of 63%,and the prevalence in disease-bearing nurseries reached as high as 90%.In comparison to 2013,there had been no improvement in the condition of seedlings affected by *** positive detection rate of citrus yellow vein clearing virus(CYVCV)was found to be 38%,with 70%of the surveyed nurseries exhibiting the *** citrus varieties identified as carriers of the disease included‘Qicheng’,‘Shatangju’,‘Wogan’,and‘Gonggan’.Nematodes were isolated from the matrix and roots of seedlings grown in both container and open field *** susceptibility of container seedlings to nematodes was found to be 36.4%,while the susceptibility of open field seedlings was 38.6%.Statistical analysis indicated no significant difference in susceptibility be

关键词： citrus diseases and pests Investigation qPCR RT-PCR Detection

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