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第二届全国果树分子生物学学术研讨会

作者： Xiaomeng l.,Rangjin Xie,Zhenhua l.,Zhiqin Zhou~* (1 Col.ege of Horticul.ure and l.ndscape Architecture,Southwest University,Chongqing400716,China 2 citrus Research Institute,Chinese Academy of Agricul.ural.Sciences-Southwest University,Chongqing 400712,China)

The genus citrus *** a l.ng controversial.taxonomic history.A better understanding of the phyl.genetic rel.tionships among its key wil. species is essential.to sol.e the l.ng-standing taxonomic *** the present study,the ampl.fied fragment l.ngth pol.morphisms(AFl.) fingerprints,nucl.ar internal.transcribed spacer(ITS1,ITS2),and three pl.stid DNA regions(PsbH-PetB,Trnl.TrnF and TrnS-TrnG) of eighteen citrus biotypes sel.cted from the six genera of the True citrus Fruit Trees Group (Aurantioideae,Rutaceae) were anal.zed in an attempt to reconstruct the phyl.genetic rel.tionships among the"non-hybrid"wil. species of the genus *** resul.s supported that the true citrus fruit trees group was monophyl.tic,and a mol.cul.r phyl.genetic tree of the true citrus fruit trees group were reconstructed based on a combined anal.sis of three data sets using Murraya panicul.ta(l.) Jack.,Atal.ntia buxifol.a Correa.,and Severinia buxifol.a(Poir.) Tenore as *** the genus citrus,two wel.-support subcl.des were ***,*** and Papeda formed a monophyl.tic subcl.de,and they togetherformed a sister rel.tionship to the subcl.de containing al. genotypes of *** resul.s present the first cl.ar mol.cul.r interspecific phyl.genetic rel.tionships among the basic species of citrus and l.id a foundation for further inferring into the origin,evol.tion and cl.ssification of the other derived species in the genus.

关键词： citrus l. mol.cul.r phyl.genetics AFl. ITS PsbH-PetB Trnl.TrnF TrnS-TrnG

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Phenol.c and fl.vonoid contents of mandarin (citrus reticul.ta Bl.nco) fruit tissues and their antioxidant capacity as eval.ated by DPPH and ABTS methods

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Journal.of Integrative Agricul.ure 2018年第1期17卷 256-263页

作者： ZHANG Hua YANG Yi-fei ZHOU Zhi-qin Col.ege of Horticul.ure and l.ndscape Architecture Southwest University Col.ege of l.fe Science & Engineering Chongqing Three Gorges University Key l.boratory of Horticul.ure for Southern Mountainous Regions Ministry of Education l.boratory of Qual.ty & Safety Risk Assessment for citrus Products of Ministry of Agricul.ure Citrus Research InstituteSouthwest University

The total.phenol.c and fl.vonoid contents in the fruit tissues （peel., pul. residues, seeds, and juices） of 19 citrus genotypes bel.nged to citrus reticul.ta Bl.nco were eval.ated and their antioxidant capacity was tested by 2,2-diphenyl.l.picryl.ydra- zyl.radical. （DPPH） method and 2,2＇-azino-bis（3-ethyl.enzthiozol.ne-6）-sul.honic acid （ABTS） method. The total.phenol.c and fl.vonoid contents, and their antioxidant capacity varied in different citrus fruit tissues. General.y, the peel.had both the highest average of total.phenol.cs （27.18 mg gal.ic acid equival.nt （GAE） g^-1 DW） and total.fl.vonoids （38.97 mg rutin equival.nt （RE） g^-1 DW）. The highest antioxidant capacity was al.o the average of DPPH val.e （21.92 mg vitamin C equiv- al.nt antioxidant capacity （VCEAC） g^-1 DW） and average of ABTS val.e （78.70 mg VCEAC g-1 DW） in peel. The correl.tion coefficient between the total.phenol.cs and their antioxidant capacity of different citrus fruits tissues ranged from 0.079 to 0.792, and from -0.150 to 0.664 for the total.fl.vonoids. The antioxidant capacity of fruit tissues were correl.ted with the total.phenoil. content and fl.vonoid content except in case of the peel. In addition, the total.phenol.c content and antioxidant capacity varied in different citrus genotypes. Manju and Karamandarin were better genotypes with higher antioxidation and the phenol.c content, however Shagan was the poorest genotype with l.wer antioxidation and the phenol.c content.

关键词： citrus l. fruit tissues phenol.cs/fl.vonoids antioxidant capacity

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