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第十届全国化学生物学学术会议

作者： Tingting Fan Yu Mao Yuyang Jiang Department of Chemistry Tsinghua University The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology the Graduate School at ShenzhenTsinghua University

Serum circulating micrornas（c-miRNAs） are emerging as new non-invasive biomarkers for breast cancer diagnosis. However, to our knowledge, there is no attempt to assess the value of branched rolling circle amplification（BRCA）, which is an isothermal amplification strategy, in serum circulating micrornas detection for the early diagnosis of breast cancer. In this study, circulating miR-16（c-miR-16）, circulating miR-21（c-miR-21）, circulating miR-155（c-miR-155）, and circulating miR-195（c-miR-195） were selected as candidate biomarkers. Furthermore, we analyzed a large cohort of serum samples from patients with early breast cancer using a simple and sensitive isothermal detection assay. Our research demonstrated that the levels of c-miR-16, c-miR-21, c-miR-155, and c-miR-195 could discriminate different histological tumor grades from healthy controls, especially for early-stage breast cancer. Our findings highlight the value of BRCA in serum circulating micrornas detection and the potential clinical use of these serum-circulating micrornas for non-invasive screening of patients with breast cancer.

关键词： Breast cancer early detection circulating micrornas BRCA

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micrornas in heart and circulation during physical exercise

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Journal of Sport and Health Science 2018年第4期7卷 433-441页

作者： Lijun Wang Yicheng Lv Guoping Li Junjie Xiao Cardiac Regeneration and Ageing Lab Institute of Cardiovascular SciencesSchool of Life ScienceShanghai UniversityShanghai 200444Chin Cardiovascular Division of the Massachusetts General Hospital and Harvard Medical School BostonMA 02215USA Corresponding author.E-mail address.junjiexiao@***

Exercise training is beneficial to the cardiovascular system. micrornas （miRNAs, miRs） are a class of conserved non-coding RNAs and play a wide-ranging role in the regulation of eukaryotic gene expression. Exercise training alters the expression levels of large amounts ofmiRNAs in the heart. In addition, circulating miRNAs appear to be regulated by exercise training. In this review, we will summarize recent advances in the regulation of miRNAs during physical exercise intervention in various cardiovascular diseases, including pathologic cardiac hypertrophy, myocardial fibrosis, ischemia-reperfusion injury, myocardial infarction, and heart failure. The regulatory role of circulating miRNAs after exercise training was also reviewed. In conclusion, miRNAs might be a valuable target for treatment of cardiovascular diseases and have great potential as biomarkers for assessment of physical performance.2018 Published by Elsevier *** behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license. （http：//***/licenses/by-nc-nd/4.0/）.

关键词： Cardiovascular diseases circulating micrornas Exercise micrornas

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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

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Journal of Animal Science and Biotechnology 2012年第2期3卷 51-59页

作者： Kang Kang Xiao Peng Jun Luo Deming Gou College of Life Science Shenzhen UniversityShenzhenGuangdong518060China College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi712100China

micrornas （miRNAs） are small noncoding RNAs （18-25 nucleotides） that regulate gene expression at the posttranscriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs i n serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction （PCR）-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps：（1） sample collection and preparation; （2） global miRNAs profiling using quantitative real-time PCR （qRT-PCR）; （3） data normalization and analysis; and （4） selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers.

关键词： biomarker circulating micrornas profiling quantitative real-time PCR

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