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Screening and functional analysis of the long-range interaction elements ofβ-globin genes

Journal of Hainan Medical University 2022年第24期28卷 1-7页

作者： XIAO Yi-shu XU Lan LIU Chun-ya DU Le SUN Yuan-tian LI Dong-na REN Li-cheng Department of Biology Hainan Medical UniversityHaikou 571199China

Objective:Studies have shown thatβ-globin gene presents a selective expression transformation mechanism during development,and its upstream locus control region(LCR)regulates the expression pattern ofβ-globin gene *** further explore the molecular network ofβ-globin gene expression regulation,other long-range regulatory elements that may be involved in the regulation ofβ-globin gene expression were screened and the dynamic regulation and transformation mechanism ofβ-globin gene was deeply ***:Promyelocytic cells were induced to differentiate by all-trans retinoic acid.β-globin gene promoter region and LCR were used as the target sites for circular chromosome conformational capture(4C)*** sequencing and regulatory element analysis,the sites interacting withβ-globin family loci were screened in the whole ***:According to the results of 4C sequencing,the sites that interact with HBD promoter region and LCR were *** by chromosome conformational capture(3C),the results were consistent with those of *** functional analysis of regulatory elements by formaldehyde-assisted separation regulatory elements and Epiregio online website showed that the screening sites AC105129.4,AL354707.17,AC078785.22 and AC021646.35 were all potential regulatory elements involved inβ-globin ***:The interaction between 4C screening site and anchor site showed the complex spatial organization ofβ-globin family loci in the nucleus.

关键词： β-Globin gene circular chromosome conformation capture Chromatin interaction chromosome conformation capture

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