检索结果-南通市图书馆

Development of chromatographic fingerprint for quality analysis of diploid and tetraploid Lonicera japonica

Journal of Traditional Chinese Medicine 2020年第1期40卷 73-82页

作者： Hu Xuan Wang Dan Pang Yuxin Wu Zhisheng Huan Hengfu Chen Zhijian Li Weidong Institute of Tropical Crop Genetic Resources Chinese Academy of Tropical Agriculture SciencesDanzhou 571737China School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100102China

OBJECTIVE:To develop the chromatographic fingerprint of Lonicera japonica(L.japonica)and evaluate the effects of polyploidy on the quality of L.japonica.METHODS:high-performance liquid chromatography(HPLC)methods used to establish the chromatographic fingerprint were developed.The quality of 11 batches of diploid L.japonica and 13 batches of tetraploid L.japonica collected from different regions across China were analyzed.The contents of five active compounds,consisting of chlorogenic acid,rutin,galuteolin,isochlorogenic acid A and quercetin,were further detected in L.japonica.RESULTS:The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L.japonica.Quantitative analysis showed that the contents of chlorogenic acid,isochlorogenic acid A,and quercetin in tetraploid plants were higher than those in diploid plants,whereas rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants.CONCLUSION:The developed HPLC method is suitable for qualitative analysis of L.japonica.Polyploidy was indicated to influence the chemical properties of L.japonica.Tetraploid L.japonica shows potential for utilization as a medicinal plant with different active components.

关键词： Lonicera Polyploidy chromatography,high pressure liquid Dermatoglyphics Phytochemicals Qualitative research

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Absorption and biotransformation of four compounds in the Guizhi decoction in the gastrointestinal tracts of rats

引用

Journal of Traditional Chinese Medicine 2019年第3期39卷 332-338页

作者： Gao Huan Zhang Lishi Song Jiannan Bai Dong College of Pharmacy Shaanxi University of Chinese Medicine Xianyang 712046 China Institute of Basic Theory of Traditional Chinese Medicine China Academy of Chinese Medical Sciences Beijing 100700 China

OBJECTIVE:To study the absorption and biotransformation of liquiritin,cinnamic acid,paeoniflorin,and glycyrrhizic acid in the Guizhi decoction (GZD) in the gastrointestinal tracts of rats.METHODS:A simple and reliable high-performance liquid chromatography method was established and validated for the analysis of the four components of GZD simultaneously in the gastrointestinal tracts of rats.Rats were randomly divided into in situ gastrointestinal loop model,in vitro anaerobic culture model,and blank control groups.All rats were fasted for 12 h and anesthetized using 20% urethane.Subsequently,the abdominal cavity of each rat was opened,and the stomach,duodenum,jejunum,ileum,cecum,and colon were ligated.For the in situ gastrointestinal loop model group,2.5 mL of GZD (1.0 g crude drug/mL,37 ℃)were injected into the gastrointestinal tract.The abdominal incision was covered with warm,wet cotton,and animals were maintained at 25 ℃.Then,we collected the gastrointestinal tract content after 1.5 h.For the in vitro anaerobic culture model group,the gastrointestinal tract contents of rats were collected and then cultured in 2.5 mL of GZD in an anaerobic environment at 25 ℃ for 24 h.For the blank control group,rats received the same volume of a normal saline solution instead of GZD.high performance liquid chromatography was used to detect the liquiritin,cinnamic acid,paeoniflorin,and glycyrrhizic acid concentrations in each group and calculate the absorption and biotransformation rates of each ingredient.RESULTS:Cinnamic acid (low polarity) was more easily absorbed by each gastrointestinal part than the higher-polarity glycosides.However,the absorption rate in the cecum was higher than that in other parts.The four compounds,cinnamic acid,liquiritin,paeoniflorin,and glycyrrhizic acid,were transformed completely within 24 h in the cecum and colon,whereas they were hardly transformed in the stomach,excluding glycyrrhizic acid.In addition,all ingredients had higher biotransformation rates in the distal small intestine than that

关键词： Absorption Cinnamates Glycyrrhizic acid Biotransformation chromatography,high pressure liquid Guizhi decoction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：