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Horticultural Plant Journal 2023年第5期9卷 1039-1054页

作者： Xinwei Wang Chunxue Shao Libo Liu Yue Wang Yaqi An Hui Li Yunwei Ding Yanping Jing Xiaojuan Li Jianwei Xiao College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083China Hebei North University ZhangjiakouHebei 075000China National Engineering Research Center of Tree Breeding and Ecological Restoration Beijing Forestry UniversityBeijing 100083China Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of EducationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast ***,many questions regarding these processes remain unanswered,particularly in perennial woody *** a model material of woody plants,poplar not only has very significant value of research,but also possesses economic and ecological *** study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 ***1C knock-out with the CRISPR/Cas9 system resulted in different albino *** fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased ***,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant ***,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin *** concluded that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar.

关键词： PtrDJ1C chloroplast development Gene expression Lignin Poplar

The rice OsV4 encoding a novel pentatricopeptide repeat protein is required for chloroplast development during the early leaf stage under cold stress

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Journal of Integrative Plant Biology 2014年第4期56卷 400-410页

作者： Xiaodi Gong Qianqian Su Dongzhi Lin Quan Jiang Jianlong Xu Jianhui Zhang Sheng Teng Yanjun Dong development Center of Plant Germplasm Resources College of Life and Environmental Sciences Shanghai Normal University Institute of Crop Sciences the Chinese Academy of Agricultural Sciences Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences the Chinese Academy of Sciences

Pentatricopeptide repeat （PPR） proteins, charac- terized by tandem arrays of a 35 amino acid motif, have been suggested to play central and broad roles in modulating the expression of organelle genes in plants. However, the molecular mechanisms of most rice PPR genes remains unclear. In this paper, we isolated and characterized a temperature-conditional virescent mutant, OsV4, in rice （Oryza sativa cultivar Jiahual （WT, japonica rice variety））. The mutant displays albino phenotype and abnormal chloroplasts at the three leaf stage, which gradually turns green after the four leaf stage at a low temperature （20℃）. But the mutant always develops green leaves and well-developed chloroplasts at a high temperature （32℃）. Genetic and molecular analyses uncovered that OsV4 encodes a novel chloroplast-targeted PPR protein including four PPR motifs. Further investigations show that the mutant phenotype is associated with changes in chlorophyll content and chloroplast development. The OsV4 transcripts only accumulate to high levels in young leaves, indicating that its expression is tissue-specific. In addition, transcript levels of some ribosomal components and plastid- encoded polymerase-dependent genes are dramatically re- duced in the albino mutants grown at 20℃. These findings suggest that OsV4 plays an important role during early chloroplast development under cold stress in rice.

关键词： chloroplast development pentatricopeptide repeat plastid-encoded polymerase rice virescent

CDE4 encodes a pentatricopeptide repeat protein involved in chloroplast RNA splicing and affects chloroplast development under low-temperature conditions in rice

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Journal of Integrative Plant Biology 2021年第10期63卷 1724-1739页

作者： Xinyong Liu Xichun Zhang Ruijie Cao Guiai Jiao Shikai Hu Gaoneng Shao Zhonghua Sheng Lihong Xie Shaoqing Tang Xiangjin Wei Peisong Hu State Key Laboratory of Rice Biology China National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 310006China Guizhou Rice Research Institute Guizhou Academy of Agricultural SciencesGuiyang 550006China

Pentatricopeptide repeat(PPR)proteins play important roles in the post-transcriptional modification of organellar RNAs in ***,the function of most PPR proteins remains ***,we characterized the rice(Oryza sativa L.)chlorophyll deficient 4(cde4)mutant which exhibits an albino phenotype during early leaf development,with decreased chlorophyll contents and abnormal chloroplasts at low-temperature(20℃).Positional cloning revealed that CDE4 encodes a P-type PPR protein localized in *** the cde4 mutant,plastid-encoded polymerase(PEP)-dependent transcript levels were significantly reduced,but transcript levels of nuclear-encoded genes were increased compared to wild-type plants at 20℃.CDE4 directly binds to the transcripts of the chloroplast genes rpl2,ndhA,and *** splicing of these transcripts was defective in the cde4 mutant at 20℃,but was normal at 32℃.Moreover,CDE4 interacts with the guanylate kinase VIRESCENT 2(V2);overexpression of V2 enhanced CDE4 protein stability,thereby rescuing the cde4 phenotype at 20℃.Our results suggest that CDE4 participates in plastid RNA splicing and plays an important role in rice chloroplast development under lowtemperature conditions.

关键词： CDE4 chloroplast development pentatricopeptide repeat(PPR)protein RNA splicing low-temperature Oryza sativa L.

The thylakoid membrane protein NTA1 is an assembly factor of the cytochrome b6f complex essential for chloroplast development in Arabidopsis

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Plant Communications 2023年第1期4卷 287-303页

作者： Na Li Wing Shing Wong Lei Feng Chunming Wang King Shing Wong Nianhui Zhang Wei Yang Yueming Jiang Liwen Jiang Jun-Xian He School of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengduSichuanChina Guangdong Provincial Key Laboratory of Applied Botany&Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement Core Botanical GardensSouth China Botanical GardenChinese Academy of SciencesGuangzhouChina

The cytochrome b_(6f)(Cyt b_(6f))complex is a multisubunit protein complex in chloroplast thylakoid membranes required for photosynthetic electron *** we report the isolation and characterization of the new tiny albino 1(nta1)mutant in Arabidopsis,which has severe defects in Cyt b_(6f) accumulation and chloroplast *** cloning revealed that the nta1 phenotype was caused by disruption of a single nuclear gene,NTA1,which encodes an integral thylakoid membrane protein conserved across green algae and *** of NTA1 completely rescued the nta1 phenotype,and knockout of NTA1 in wild-type plants recapitulated the mutant *** of NTA1 function severely impaired the accumulation of multiprotein complexes related to photosynthesis in thylakoid membranes,particularly the components of Cyt b_(6f).NTA1 was shown to directly interact with four subunits(Cyt b6/PetB,PetD,PetG,and PetN)of Cyt b_(6f) through the DUF1279 domain and C-terminal sequence to mediate their *** together,our results identify NTA1 as a new and key regulator of chloroplast development that plays essential roles in assembly of the Cyt b_(6f) complex by interacting with multiple Cyt b_(6f) subunits.

关键词： albino mutant chloroplast development cytochrome b6f complex assembly factor Arabidopsis thaliana

Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development

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Journal of Integrative Plant Biology 2023年第7期65卷 1687-1702页

作者： Jie Lan Qibing Lin Chunlei Zhou Xi Liu Rong Miao Tengfei Ma Yaping Chen Changling Mou Ruonan Jing Miao Feng Thanhliem Nguyen Yulong Ren Zhijun Cheng Xin Zhang Shijia Liu Ling Jiang Jianmin Wan State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Pentatricopeptide repeat(PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice(Oryza sativa), the detailed molecular functions of many PPR proteins remain ***, we characterized a rice young leaf white stripe(ylws) mutant, which has defective chloroplast development during early seedling ***-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear-and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2,and rps12, and editing of ndhA, ndhB, and rps14transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development.

关键词： chloroplast development PPR protein ribosome biogenesis RNA splicing

Os DXR interacts with Os MORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice

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Journal of Integrative Agriculture 2023年第3期22卷 669-678页

作者： CAO Peng-hui WANG Di GAO Su LIU Xi QIAO Zhong-ying XIE Yu-lin DONG Ming-hui DU Tan-xiao ZHANG Xian ZHANG Rui JI Jian-hui Institute of Agricultural Sciences in Taihu Area of Jiangsu Suzhou 215155P.R.China Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu Huai’an 223001P.R.China School of Life Sciences Huaiyin Normal UniversityHuai’an 223300P.R.China Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake Regional Cooperative Innovation Center for Modern Agriculture and Environmental ProtectionHuaiyin Normal UniversityHuai’an 223300P.R.China

Plant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice. OsDXR knock-out lines displayed the albino phenotype and could not complete the whole life cycle process. OsDXR was highly expressed in rice leaves, and subcellular localization indicated that OsDXR is a chloroplast protein. Many genes involved in chlorophyll biosynthesis and chloroplast development were differentially expressed in the OsDXR knock-out lines compared to the wild ***, we found that the RNA editing efficiencies of ndhA-1019 and rpl2-1 were significantly reduced in the OsDXR knock-out lines. Furthermore, OsDXR interacted with the RNA editing factor OsMORF1 in a yeast two-hybrid screen and bimolecular fluorescence complementation assay. Finally, disruption of the plastidial 2-C-methyl-derythritol-4-phosphate pathway resulted in defects in chloroplast development and the RNA editing of chloroplast genes.

关键词： rice OsDXR chloroplast development RNA editing OsMORF1

SNOWY COTYLEDON 2 Promotes chloroplast development and Has a Role in Leaf Variegation in Both Lotus japonicus and Arabidopsis thaliana

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Molecular Plant 2017年第5期10卷 721-734页

作者： Nicola Zagari Omar Sandoval-lbanez Niels Sandal Junyi Su Manuel Rodriguez-Concepcion Jens Stougaard Mathias Pribil Dario Leister Pablo Pulido Plant Molecular Biology Department of Biology I Ludwig-Maximilians-Universitat Munchen 82152 Planegg-Martinsried Germany Copenhagen Plant Science Center University of Copenhagen 1871 Frederiksberg C Denmark Research and Innovation Center Fondazione Edmund Mach via E. Mach 1 38010 San Michele all'Adige Italy Centre for Carbohydrate Recognition and Signalling Department of Molecular Biology and Genetics Aarhus University Gustav Wieds Vej 10 8000 Aarhus C Denmark Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB Campus UAB Bellaterra 08193 Barcelona Spain

Plants contain various factors that transiently interact with subunits or intermediates of the thylakoid multiprotein complexes, promoting their stable association and integration. Hence, assembly factors are essential for chloroplast development and the transition from heterotrophic to phototrophic growth. Snowy cotyledon 2 （SCO2） is a DNAJ-like protein involved in thylakoid membrane biogenesis and interacts with the light-harvesting chlorophyll-binding protein LHCBI. In Arabidopsis thaliana, SCO2 function was previ- ously reported to be restricted to cotyledons. Here we show that disruption of SC02 in Lotus japonicus results not only in paler cotyledons but also in variegated true leaves. Furthermore, smaller and pale- green true leaves can also be observed in A. thaliana sco2 （atsco2） mutants under short-day conditions. In both species, SCO2 is required for proper accumulation of PSlI-LHCll complexes. In contrast to other variegated mutants, inhibition of chloroplastic translation strongly affects L. japonicus sco2 mutant devel- opment and fails to suppress their variegated phenotype. Moreover, inactivation of the suppressor of variegation AtClpR1 in the atsco2 background results in an additive double-mutant phenotype with variegated true leaves. Taken together, our results indicate that SCO2 plays a distinct role in PSll assembly or repair and constitutes a novel factor involved in leaf variegation.

关键词： SCO2 DNAJ-like photosynthesis assembly factor variegation chloroplast development

A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed

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Journal of Integrative Agriculture 2024年第5期23卷 1481-1493页

作者： Qianwei zhang Yuanyi Mao Zikun Zhao Xin Hu Ran Hu Nengwen Yin Xue Sun Fujun Sun Si Chen Yuxiang jiang Liezhao Liu Kun Lu Jiana Li Yu Pan Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Southwest UniversityChongqing 400715China Academy of Agricultural Sciences Southwest UniversityChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and *** this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic *** BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and *** with this,BnGLK1a-RNAi lines of *** display hypersensitivity to the abscisic acid(ABA)***,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild *** propose that BnGLK1a could be a potential target in breeding for improving rapeseed *** results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.

关键词： Brassica napus BnGLK1a chloroplast development photosynthetic efficiency yield

OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development

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Journal of Integrative Agriculture 2023年第4期22卷 972-980页

作者： CHEN Chang-zhao WANG Ya-liang HE Meng-xing LI Zhi-wen SHEN Lan LI Qing REN Deyong HU Jiang ZHU Li ZHANG Guang-heng GAO Zhen-yu ZENG Da-li GUO Long-biao QIAN Qian ZHANG Qiang State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004P.R.China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 310006P.R.China

Photosynthesis occurs mainly in chloroplasts,whose development is regulated by proteins encoded by nuclear *** them,pentapeptide repeat(PPR)proteins participate in organelle RNA *** there are more than 450 members of the PPR protein family in rice,only a few affect RNA editing in rice *** editing technology has created new rice germplasm and mutants,which could be used for rice breeding and gene function *** study evaluated the functions of OsPPR9 in chloroplast RNA editing in *** osppr9 mutants were obtained by CRISPR/Cas9,which showed yellowing leaves and a lethal phenotype,with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related *** addition,loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182,rpoC2-C4106,rps14-C80,and ndhB-C611 RNA editing sites,which affects chloroplast growth and development in *** data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in ***,the OsPPR9 protein was shown to interact with OsMORF2 and ***,our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice.

关键词： rice(Oryza sativa L.) PPR protein chloroplast development RNA editing

Expression of the Thylakoid Membrane Localized PPF1 in Transgenic Arabidopsis Affects chloroplast development

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Acta Botanica Sinica 2002年第11期44卷 1314-1320页

作者：徐云剑王大勇朱玉贤北京大学蛋白质工程及植物基因工程国家重点实验室北京100871

PPF1 is a vegetative growth related gene that encodes a putative membrane protein having high homology with Arabidopsis chloroplast thylakoid protein ALB3. Immunoelectron microscopic assay showed that PPF1 was mainly localized in the thylakold membrane and was highly expressed in well-developed chloroplasts of short day (SD) grown G2 pea while having a very low abundance in chloroplasts of long day (LD) grown plants two weeks after flowering. Comparison of the leaf senescence processes in transgenic Arabidopsis and wild type plants revealed that overexpression of PPF1 delayed leaf senescence, while the depression of its Arabidopsts homologue (ALB3) with PPF1 antisense mRNA accelerated leaf senescence obviously. Ultrastructural analysis of transgenic Arabidopsis plants showed that when PPF1 was overexpressed in Arabidopsis, the chloroplasts were bigger and had much more grana and stroma thylakoid membranes than those of wild type plants. On the contrary, when PPF1 was expressed in antisense orientation to reduce the level of PPF1 homologue in Arabidopsis, the transgenic plants had smaller chloroplasts With less grana. and poorly developed thylakoid membrane systems. These results suggested that the developmental status of chloroplasts was positively correlated with the level of PPF1 or its Arabidopsts homologue, ALB3. Our results suggested that PPF1 gene might regulate plant development by controlling chloroplast development.

关键词： PPF1 immunolocalization chloroplast development senescence