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Function of chloride intracellular channel 1 in gastric cancer cells

World Journal of Gastroenterology 2012年第24期18卷 3070-3080页

作者： Peng-Fei Ma Jun-Qiang Chen Zhen Wang Jin-Lu Liu Bo-Pei Li Department of Gastrointestinal Surgery the First Affili- ated Hospital of Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China

AIM:To investigate the effect of chloride intracellular channel 1(CLIC1) on the cell proliferation,apoptosis,migration and invasion of gastric cancer ***:CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction(RT-PCR).Four segments of small interference RNA(siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics ***1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 *** transfected efficiency was observed under fluorescence *** transfection,mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein *** was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow *** membrane transwell chamber and Matrigel were used for the detection of the changes of invasion and migration of the two cell ***:In gastric cancer cell lines SGC-7901 and MGC-803,CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and *** of cells transfected with CLIC1 siRNA3 was enhanced notably,and the highest proliferation rate was 23.3%(P = 0.002) in SGC-7901 and 35.55%(P = 0.001) in MGC-803 cells at 48 *** G2/M phase proportion increased,while G0/G1 and S phase proportions *** apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells(62.24%,P = 0.000) and MGC-803 cells(52.67%,P = 0.004).Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31%(P = 0.000) and 33.62%(P = 0.001) in SGC-7901 and 40.74%(P = 0.000) and 29.26%(P = 0.002) in ***,there was no significant difference between the mock group cells and the negative control group cells.

关键词： chloride intracellular channel 1 Gastric car-cinoma Small interference RNA Apoptosis Invasion Migration

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