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Control Efficacy of Streptomyces sp. SY-L12 and chitooligosaccharide on Kiwifruit Bacterial Canker, Pseudomonas syringae pv. actinidiae

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Plant Diseases and Pests 2017年第4期8卷 17-20页

作者： Wang Fang Wei Tingting Xin Xuecheng Jiao Jiao Li Jiguang Fan Jinzhe Zhou Fan Shenyang Research Institute of Chemical Industry Co.Ltd. Shenyang 110021China

[ Objective] The paper was to develop a priority scheme for prevention and control of kiwifruit bacterial canker with biological agents. [ Method ] The inhibitory effect of Streptomyces sp. SY-L12 on the growth of Pseudomonas syringae pv. actinidiae were assessed using liquid co-culture and inhibition zone meth- ods. The control efficiency of Streptomyces sp. fermentation broth against kiwifruit bacterial canker was studied via potted trial. [ Result ] As Streptomyces sp. SY- L12 was incubated for 24 h, the growth inhibition rate of its fermentation broth on P. syr/ngae pv. actinidiae was 94.0%. The control efficiency of fermentation broth against P. syringae pv. actinidiae was the best of 85.4% in potted trial when Streptomyces sp. SY-L12 grew at 28~C, 150 rpm for 8 -9 d, better than that of control agent copper hydroxide. The control efficiency was improved as the agent was mixed with 1 mg/L chitooligosaccharide. [ Conclusion] Streptomyces sp. SY-L12 could effectively inhibit the growth of P. syr/ngae pv. actinidiae, and can be developed as biological agent against kiwifruit bacterial canker.

关键词： Streptomyces sp. Kiwlfruit bacterial canker Antimicrobial activity Biological control chitooligosaccharide

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Mechanism study and application of a plant elicitor-chitooligosaccharide

Mechanism study and application of a plant elicitor-chitooli...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Heng Yin,Xiaoming Zhao,Yuguang Du~* Liaoning Provincial Key laboratory of Carbohydrates,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023.

Chitosan is one of the most abundant carbohydrate biopolymers in the world. chitooligosaccharides prepared from chitosan is a potent plant *** efficacy of chitooligosaccharides on plant disease control and stress resistance was reported by numerous *** plant growth promoters and bio-pesticides were developed and used in several provinces in China. Based on our experiments and other literature,we conclude that there are six steps in the process of chitooligosaccharides activated plant immunity:chitooligosaccharides signal reception;secondary signal messengers(NO,HO...) activation;plant hormones(SA,JA, ET...) accumulation;defense signal transcuction;defense-related genes and proteins expression,defense reaction(secondary metabolites production,PR proteins expression, callose accumulation...).

关键词： chitooligosaccharide chitosan elicitor plant immunity plant defense

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chitooligosaccharides from squid pen prepared using different enzymes:characteristics and the effect on quality of surimi gel during refrigerated storage

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食物生产加工与营养(英文) 2019年第1期1卷 93-102页

作者： Avtar Singh Soottawat Benjakul Thummanoon Prodpran Department of Food Technology Faculty of Agro-IndustryPrince of Songkla UniversityHat YaiSongkhla 90110Thailand Department of Material Product Technology Faculty of Agro-IndustryPrince of Songkla UniversityHat YaiSongkhla 90110Thailand

chitooligosaccharides(COS)from squid pen produced using amylase,lipase and pepsin were *** produced by 8%(w/w)lipase(COS-L)showed the maximum FRAP and ABTS radical scavenging activity than those prepared using other two ***-L had the average molecular weight(MW)of 79 kDa,intrinsic viscosity of 0.41 dL/g and water solubility of 49%.DPPH,ABTS radical scavenging activities,FRAP and ORAC of COS-L were 5.68,322.68,5.66 and 42.20μmol TE/g sample,*** chelating activity was 2.58μmol EE/g *** antibacterial activity,minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of COS-L against the targeted bacteria were in the range of 0.31–4.91 mg/mL and 0.62–4.91 mg/mL,*** surimi gel added with 1%(w/w)COS-L showed the lower PV,TBARS and microbial growth during 10 days of storage at 4°***-L from squid pen could inhibit lipid oxidation and extend the shelf-life of refrigerated sardine surimi gel.

关键词： Chitosan Squid pen chitooligosaccharide Sardine surimi Shelf-life extension

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Adsorption characteristics of chitooligosaccharides onto activated charcoal in aqueous solutions

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Journal of Oceanology and Limnology 2020年第2期38卷 342-350页

作者： YU Yu LI Kecheng Marine Science and Engineering College Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China

To investigate the adsorption characteristics of chitooligosaccharides in solution onto activated charcoal,we studied the optimal adsorption conditions and the adsorption mechanisms of the chitooligosaccharides onto activated charcoal,which will greatly promote the application of activated charcoal in the chitooligosaccharides separation and *** studied the effects of particle size of activated charcoal,pH of solution,contact time,temperature,and initial concentration of chitooligosaccharides on the adsorption behavior in batch mode *** charcoal in fine particle size showed a high uptake of *** alkaline solution(pH 8–9)was the most favorable to the *** adsorption equilibrium after 60 min was established,which followed a pseudo-second-order kinetic *** adsorption capacity(Q_(max))reached 0.195 g/g(chitooligosaccharides/activated charcoal)at 298 *** adsorption was temperature-insensitive,and the adsorption isotherms could be best described by the Langmuir *** adsorbed on activated charcoal could be desorbed in 50%ethanol solution in combination with an acidic condition(pH 2),reaching desorption efficiency of 96.0%.These findings are of great significance for the production and purification of amino oligosaccharides including chitooligosaccharides using activated charcoal.

关键词： chitooligosaccharide adsorption activated charcoal kinetics isotherm

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Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds

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Marine Life Science & Technology 2019年第1期1卷 95-103页

作者： Xiaodan Fu Lin Zhu Li Li Tan Zhang Meng Li Haijin Mou College of Food Science and Engineering Ocean University of ChinaNo.5 Yushan RoadQingdao 266003China

Production of chitosan and its derivatives by traditional methods involves the excessive use of a reaction solution comprisedof sodium hydroxide and hydrochloric acid. Waste water resulting from this process has lim让ed the application of chitosanas a fertilizer as the process causes serious environmental pollution. Specifically, the resulting waste water contains highlevels of dissolved nitrogen and minerals from shrimp shells. In this study, an eco-friendly method was established to produce chitooligosaccharides (COS) with different degrees of deacetylation (DDAs) from shrimp shell waste. At a solid-tosolventratio of 1:6, the degree of demineralization was above 90% with the treatment of 30 g-L_1 H3PO4, and the degree ofdeproteinization was above 80% when treated with 30 g-L_1 KOH at 70 °C. Chitosans with different DDAs were obtainedby microwave-assisted KOH metathesis and the COS with Mw approximately 1500 Da were then prepared by oxidativedegradation. In summary, 33.73 kg H3PO4,12.77 kg, and 241.31 kg KOH were supplied during the processes of demineralization,deproteinization, and deacetylation of 100 kg shrimp shell waste, respectively. The process water was totally recycled,demonstrating that the shrimp shell could be wholly transformed into fertilize The entire process created a product withthe fractions of N:P2O5:K2O:COS = 7.94:24.44:10.72:1 &27. The test on the germination promotion of wheat seeds revealedthat the COS with 72」2% DDA significantly promoted germination. This work demonstrated the use of an eco-friendlypreparation method of COS with a specific degree of deacetylation that can be applied as a fertilizer.

关键词： chitooligosaccharide Deacetylation degree Phosphoric acid Potassium hydroxide Germination promotion

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Signaling events during initiation of arbuscular mycorrhizal symbiosis

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Journal of Integrative Plant Biology 2014年第3期56卷 250-261页

作者： Alexa M.Schmitz Maria J.Harrison Boyce Thompson Institute for Plant Research Plant Pathology and Plant-Microbe Biology Cornell University

Under nutrient-limiting conditions, plants will enter into symbiosis with arbuscular mycorrhizal （AM） fungi for the enhancement of mineral nutrient acquisition from the surrounding soil. AM fungi live in close, intracellular association with plant roots where they transfer phosphate and nitrogen to the plant in exchange for carbon. They are obligate fungi, relying on their host as their only carbon source. Much has been discovered in the last decade concerning the signaling events during initiation of the AM symbiosis, including the identification of signaling molecules generated by both partners. This signaling occurs through symbiosis-specific gene products in the host plant, which are indispensable for normal AM development. At the same time, plants have adapted complex mechanisms for avoiding infection by pathogenic fungi, including an innate immune response to general microbial molecules, such as chitin present in fungal cell walls. How it is that AM fungal colonization is maintained without eliciting a defensive response from the host is still uncertain. In this review, we present a summary of the molecular signals and their elicited responses during initiation of the AM symbiosis, including plant immune responses and their suppression.

关键词： Arbuscular mycorrhizal fungi chitin chitooligosaccharide cutin LysM MAMP-triggered immunity Myc-LCO Nod factor strigolactones symbiosis

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壳寡糖的纯化及质谱表征

壳寡糖的纯化及质谱表征

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2005年全国有机质谱学术交流会

作者：石磊韩丽华刘文祥刘晓梅谭岩吉林大学公共卫生学院上海市肿瘤研究所流行病学研究室吉林大学第一医院

甲壳素(chitin),化学名为β-(1,4)-2-乙酰氨基-2-脱氧-D葡聚糖,是自然界中一种丰富的生物质。甲壳素经化学方法脱乙酰后得到的壳聚糖(chitosan)溶解性和物化性质都有所改善,具有抗肿瘤、抗感染、抗炎、抗衰老等多种生物活性。壳寡糖(chi... 详细信息

甲壳素(chitin),化学名为β-(1,4)-2-乙酰氨基-2-脱氧-D葡聚糖,是自然界中一种丰富的生物质。甲壳素经化学方法脱乙酰后得到的壳聚糖(chitosan)溶解性和物化性质都有所改善,具有抗肿瘤、抗感染、抗炎、抗衰老等多种生物活性。壳寡糖(chitooligosaccharide)是壳聚糖经酶法或化

关键词： chitooligosaccharide Degree of polymer (DP) MALDI-FT MS

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