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Virus-induced gene silencing(VIGS)in chinese narcissus and its use in functional analysis of NtMYB3

Horticultural Plant Journal 2021年第6期7卷 565-572页

作者： Ping Zhou Jiayu Peng Minjie Zeng Lixuan Wu Yuxin Fan Lihui Zeng College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 35002China College of Horticulture Nanjing Agriculture UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional *** present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant ***,the VIGS system in chinese narcissus had not been reported *** this paper,the first use of the TRV-VIGS system in chinese narcissus is described in *** injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus *** the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully ***,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of *** results indicate that the TRV-VIGS system has been successfully established in chinese narcissus and successfully applied to the functional study of NtMYB3.

关键词： chinese narcissus VIGS Tobacco rattle virus(TRV) NtPDS NtMYB3

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Isolation and characterization of the AGAMOUS homologous gene NTAG in chinese narcissus (narcissus tazetta var. chinensis Roem)

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Forestry Studies in China 2006年第1期8卷 21-26页

作者： Wang Zheng-ke Gao Jian Li Lu-bin Peng Zhen-hua Institute of Forest Research Chinese Academy of Forestry Beijing 100091 P. R. China International Center for Bamboo and Rattan Key Laboratory for Science and Technology in Bamboo and Rattan State Forestry Administration Beijing 100102 P. R. China

Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. chinese narcissus （narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at lower temperatures during the chinese Spring Festival, is a traditional chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction （RT-PCR） with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5＇ and the 3＇ regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends （RACE）. The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG （Hyacinthus orientalis）. Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots.

关键词： NTAG (narcissus tazetta var. chinensis Roem AGAMOUS) cDNA amplified fragment length polymorphism chinese narcissus

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维普期刊数据库

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The Relationship between Dormancy of chinese narcissus （narcissustazetta L. var. chinensis Roem） and Temperature and Phytohormone

The Relationship between Dormancy of Chinese Narcissus （Nar...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Xiaofang Li*, Yang Wang, Xinjie Deng, Lijun Xiong School of Life Science, East China Normal University, Shanghai 200062

＜正＞The dormancy of chinese narcissus （narcissustazetta L. var. chinensis Roem） is associated with high temperature. However, little information is known about it. Here the dormancy of Chongming narcissus and its

关键词： chinese narcissus bulb dormancy Temperature Ethylene GA3

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