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CSCS:a chromatin state interface for chinese spring bread wheat

aBIOTECH 2021年第4期2卷 357-364页

作者： Xiaojuan Ran Tengfei Tang Meiyue Wang Luhuan Ye Yili Zhuang Fei Zhao Yijing Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences300 Fenglin RoadShanghai 200032China University of the chinese Academy of Sciences Beijing 100049China School of Life Science Henan UniversityKaifeng 457000HenanChina

A chromosome-level genome assembly of the bread wheat variety chinese spring(CS)has recently been ***-wide identification of regulatory elements(REs)responsible for regulating gene activity is key to further mechanistic *** epigenetic activity can reflect RE activity,defining chromatin states based on epigenomic features is an effective way to detect ***,we present the web-based platform chinese spring chromatin state(CSCS),which provides CS chromatin signature *** indudes 15 recently published epigenomic data sets including open chromatin and major chromatin marks,which are further partitioned into 15 distinct chromatin *** curates detailed information about these chromatin states,with trained self-organization mapping(SOM)for segments in all chromatin states and JBrowse visualization for genomic regions or *** analysis for genomic regions or genes,GO analysis for genes and SOM analysis for new epige-nomic data sets are also integrated into *** summary,the CSCS database contains the combina-torial patterns of chromatin signatures in wheat and facilitates the detection of functi onal elements and further clarification of regulatory *** illustrate how CSCS enables biological insights using one example,demonstrating that CSCS is a highly useful resource for intensive data *** is available at http://***/CSCS/.

关键词： Bread wheat chinese spring Chromatin state Epigenetics Database

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“中国春”(chinese spring)小麦的DNA

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世界农业 1982年第10期 65-65页

此照片在电子显微镜下拍摄。因图中的DNA呈现C.S.形,正是“中国春”的英文缩写(系偶然巧合),在本刊编者访英吋,英国植物育种研究所细胞遗传室主任***博士特赠本刊。

关键词：中国春英文缩写构词法麦类作物小麦 chinese spring DNA

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QTL mapping for grain number per spikelet in wheat using a high-density genetic map

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The Crop Journal 2021年第5期9卷 1108-1114页

作者： Yu Lin Xiaojun Jiang Haiyan Hu Kunyu Zhou Qing Wang Shifan Yu Xilan Yang Zhiqiang Wang Fangkun Wu Shihang Liu Caixia Li Mei Deng Jian Ma Guangdeng Chen Yuming Wei Youliang Zheng Yaxi Liu State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China WenjiangChengdu 611130SichuanChina Triticeae Research Institute Sichuan Agricultural UniversityWenjiangChengdu 611130SichuanChina School of Life Science and Technology Henan Institute of Science and TechnologyXinxiang 453003HenanChina College of Resources Sichuan Agricultural UniversityWenjiangChengdu 611130SichuanChina

Grain number per spikelet (GNS) is a key determinant of grain yield in wheat.A recombinant inbred line population comprising 300 lines was developed from a cross between a high GNS variety H461 and chinese spring from which the reference genome assembly of bread wheat was *** parents and the recombinant inbred lines were genotyped using the wheat 55K single nucleotide polymorphism(SNP) array.A high-density genetic map containing 21,197 SNPs was *** markers covered each of the 21 chromosomes with a total linkage distance of 3792.71 c *** of these markers in this linkage map were highly consistent with their physical locations in the genome assembly of chinese *** two parents and the whole RIL population were assessed for GNS in two consecutive years at two different *** on multi-environment phenotype data and best liner unbiased prediction values,three quantitative trait loci (QTL) for GNS were *** of them located on chromosomes 2B and the other two on *** variation explained by these loci varied from 3.07%to26.57%.One of these QTL,***-2D-2,was identified in each of all trials *** on the best linear unbiased prediction values,this locus explained 19.59%–26.57%of phenotypic variation.A KASP(Kompetitive Allele-Specific PCR) marker closely linked with this locus was generated and used to validate the effects of this locus in three different genetic *** identified QTL and the KASP marker developed for it will be highly valuable in fine-mapping the locus and in exploiting it for markerassisted selection in wheat breeding programs.

关键词： chinese spring Grain number per spikelet High-density map KASP Quantitative trait locus

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