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Methionine deficiency and its hydroxy analogue influence chicken intestinal 3-dimensional organoid development

Animal Nutrition 2022年第1期8卷 38-51页

作者： Youli Wang Qihang Hou Yuqin Wu Yanwei Xu Yan Liu Jing Chen Lingling Xu Yuming Guo Shuai Gao Jianmin Yuan State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China Key Laboratory of Animal Gene Breeding and Reproductivity National Engineering Laboratory of Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China Beijing Dafa Chia Tai Co.Ltd. Beijing101206China Sichuan New Hope Liuhe Co.Ltd Chengdu610100China

Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal *** intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal ***,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid *** results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt *** found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth ***,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of *** glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet *** serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine *** serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth ***,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid *** on the chicken intestinal organoid model,we found that methionine deficienc

关键词： chicken intestinal organoid chicken serum Methionine

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