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The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomicforce microscopy

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Cell Research 1999年第4期9卷 255-260页

作者： ZHAO HUI YI ZHANG SHU BING ZHANG CHUJIANG QI YE HE MIN QIAN LI RUO LANQIAN( Shanghai Institute of Cell Biology, Chinese Academyof Sciences, Shanghai 200031, China)( Shanghai Institute of Nuclear Research, ChineseAcademy of Sciences, Shanghai 201800, Shanghai Institute of Nuclear Research Chinese Academy of Sciences Shanghai 201800 China

INTRODUCTIONAlleukaryoticchromosomesconsistsofaregularlyrepeatingprotein--DNAcomplexcalledthenucleosome(Kornberg,1977)[******... 详细信息

INTRODUCTIONAlleukaryoticchromosomesconsistsofaregularlyrepeatingprotein--DNAcomplexcalledthenucleosome(Kornberg,1977)[******...

关键词： Nucleosome core particle chicken erythrocytes atomic force microscopy.

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Toxoplasma gondii adhesion and apoptosis of chicken erythrocytes

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Animal Diseases 2023年第3期3卷 178-187页

作者： Chenghuan Li Xiaohan Zhang Xiaoyu Sang Yanhong He Saeed El-Ashram Yingying Ding Tiantian Jiang Na Yang Key Laboratory of Livestock Infectious Diseases in Northeast China Ministry of EducationKey Laboratory of ZoonosisCollege of Animal Science and Veterinary MedicineShenyang Agricultural UniversityShenyangChina Faculty of Science Kafrelsheikh UniversityKafr El-SheikhEgypt Department of Pediatrics School of MedicineUniversity of CaliforniaLa JollaSan DiegoCAUSA

Toxoplasma gondi is thought to infect all nucleated cells in warm-blooded animals,including poultry,mammals,and ***,it is unclear whether *** can infect chicken erythrocytes due to the nucleated nature of these *** to the special role of chicken erythrocytes in innate immunity,we investigated the cell-cell interaction between *** and erythrocytes to elucidate the role of chicken erythrocytes in *** *** apoptosis was analyzed by transwell assay and flow *** immunofluorescence method was used to examine the reorganization of vimentin during *** infection in both Vero cells and chicken *** reorganization of actin was evaluated to further examine the invasion capacity of tachyzoites on chicken erythrocytes during *** discovered that *** can adhere to but not invade chicken erythrocytes and eventually cause apoptosis in chicken *** tachyzoites were cocultured with chicken erythrocytes in vitro,the transcrip-tional levels of *** MIC3,ROP16,and ROP18 were significantly *** addition,the rearrangement of host cell vimentin,a type Il cytoskeleton protein regulated by *** infection,was not ***,the parasite-induced ring-shaped actin structure was not formed in the host-parasite ***(RH strain)tachyzoites pref-erentially invaded Vero cells and replicated in chicken blood monocytes,but they were not found in chicken *** findings showed that although *** could attach to the surface of chicken erythrocytes,but couldn't invade ***,we found that the *** secretome,lysates,and intact tachyzoites could cause apoptosis of chicken erythrocytes,which suggested a complex mechanism involved in the apoptosis of chicken erythrocytes induced by *** study elucidated that *** could not infect nucleated chicken erythrocytes and enriched our understanding of the transmission mechanism of *** am

关键词： Toxoplasma gondi chicken erythrocytes Invasion Apoptosis

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