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Inorganic Elemental Determinations of Marine Traditional Chinese Medicine Meretricis concha from Jiaozhou Bay:The construction of Inorganic Elemental Fingerprint Based on chemometric analysis

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Journal of Ocean University of China 2016年第2期15卷 357-362页

作者： SHAO Mingying LI Xuejie ZHENG Kang JIANG Man YAN Cuiiwe LI Yantuan School of Medicine and Pharmacy Ocean University of ChinaQingdao 266003P.R.China Hospital of Ocean University of China Qingdao 266003P.R.China Qingdao Municipal Medical Group Qingdao 266011P.R.China Coliege of Marine Life Science Ocean University of ChinaQingdao 266003P.R.China

The goal of this paper is to explore the relationship between the inorganic elemental fingerprint and the geographical origin identification ofMeretricis concha, which is a commonly used marine traditional Chinese medicine （TCM） for the treatment of asthma and scald bums. For that, the inorganic elemental contents ofMeretricis concha from five sampling points in Jiaozhou Bay have been determined by means of inductively coupled plasma optical emission spectrometry, and the comparative investigations based on the contents of 14 inorganic elements （A1, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Se and Zn） of the samples from Jiaozhou Bay and the previous reported Rushan Bay were performed. It has been found that the samples from the two bays are ap- proximately classified into two kinds using hierarchical cluster analysis, and a four-factor model based on principle component analysis could explain approximately 75% of the detection data, also linear discriminant analysis can be used to develop a prediction model to distinguish the samples from Jiaozhou Bay and Rushan Bay with accuracy of about 93%. The results of the present investi- gation suggested that the inorganic elemental fingerprint based on the combination of the measured elemental content and chemom- etric analysis is a promising approach for verifying the geographical origin ofMeretricis concha, and this strategy should be valuable for the authenticity discrimination of some marine TCM.

关键词： Meretricis concha marine traditional Chinese medicine inorganic elemental fingerprint chemometric analysis

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Estimation of Total Phenolic Compounds and Non-Targeted Volatile Metabolomics in Leaf Tissues of American Chestnut (<i>Castanea dentata</i>), Chinese Chestnut (<i>Castanea mollissima</i>) and the Backcross Breeding Generations

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Journal of Agricultural Chemistry and Environment 2021年第2期10卷 222-256页

作者： Jinyan She Chathuri U. G. Mohottige Mary King Yi Jiang Matt Mlsna Stacy Clark Richard Baird Todd Mlsna Department of Chemistry Mississippi State University Starkville USA Department of Civil and Environmental Engineering Mississippi State University Starkville USA Department of Agriculture Forest Service Southern Research Station Knoxville USA Department of Biochemistry Molecular Biology Entomology & Plant Pathology Mississippi State University Starkville USA

The American chestnut (Castanea dentata) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by chestnut blight caused by the Ascomycetous fungus Cryphonectria parasitica. Identification of compounds specific to species and backcross hybrids may help further refine disease resistance breeding and testing. Phenolic compounds produced by plants are significant to their defense mechanisms against fungal pathogens. Therefore, an analytical platform has been developed to estimate the total phenolic content in leaf tissues of the American chestnut, Chinese chestnut (Castanea mollissima), and their backcross breeding generations (B3F2 and B3F3) using the Folin-Ciocalteu reagent assay with UV/Vis spectrophotometry which may be used to predict blight resistance. Adsorption (765 nm) results from leaf tissue extraction in methanol/water (95%:5% v/v) and pH 2, show that the variations among these four tree species are significant (ANOVA p = 2.3 × 10-7). The kinetics of phenolic compound solid-liquid extraction was elaborated using Peleg, second order, Elovich, and power law models. In addition, extensive analysis using headspace solid phase microextraction (SPME) gas chromatography and mass spectrometry was conducted to identify volatile organic compounds (VOCs) from the leaf of American chestnut, Chinese chestnut, and their backcross hybrids B3F2 and B3F3. A total of 67 VOCs were identified among all chestnut types. Many of the metabolites associated with the Chinese chestnut have been reported to have antifungal properties, whereas the native and hybrid American chestnut metabolites have not. Most of the antifungal metabolites showed the strongest efficacy towards the Ascomycota phylum. A partial least squares discriminant analysis (PLS-DA) model (R2

关键词： Total Phenolic Compounds Plant VOCs HS-SPME-GC/MS chemometric analysis Chestnut Tree Hybrids

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Flavonoid Contents and Antioxidant Activity in Fruit,Vegetables and Other Types of Food

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Agricultural Sciences 2015年第4期6卷 426-435页

作者： Juliana Cristina Pereira Calado Paula Adriana Albertao Erica Aparecida de Oliveira Mario Henrique Sisto Letra Alexandra Christine Helena Frankland Sawaya Maria Cristina Marcucci Post-Graduated School in Pharmacy Anhanguera University of Sao Paulo(UNIAN-SP)Sao PauloBrazil Department of Plant Biology and Pharmacy Course Institute of BiologyUniversidade Estadual de CampinasUNICAMPCampinasBrazil

Flavonoids are a class of natural polyphenolic compounds which cannot be synthesized by humans. These substances possess a series of biological properties, acting on biological systems as antioxidants. The purpose of this study was to analyze the properties of certain foods, determining the total flavonoids as well as their antioxidant activity and fat concentration. We evaluated several foods purchased at the local market, with respect to its antioxidant activity, using two experimental models, the discoloration of DPPH˙ radical and ABTS-. Some foods such as pitanga showed antioxidant activity. The lipid content of fatty foods like aaí, cacao and cupuaú was determined. Other foods, including pitanga, aaí, cacao and cupuaú, were evaluated for flavonoid content and antioxidant activity using multivariate statistical analysis (PCA) as a statistical tool to evaluate the correlation between these two parameters. As samples with ED50 up to 500 μg/mL show promising antioxidant activity, several Brazilian fruit and vegetables could be consumed to this end, with a good correlation between flavonoid content and antioxidant activity in most samples. The daily dose of different types of food for antioxidant activity has been calculated based on these results.

关键词： Amount of Lipids Antioxidant Activity chemometric analysis Fat Content Flavonoids Food Fruit Vegetables

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