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chemical fingerprint and Anti-Sickling Activity of Rosmarinic Acid and Methanolic Extracts from Three Species of Ocimum from DR Congo

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Journal of Biosciences and Medicines 2016年第1期4卷 59-68页

作者： Dorothée D. Tshilanda Paulin K. Mutwale Damase V. N. Onyamboko Philippe B. Babady Philippe V. Tsalu Damien S. T. Tshibangu Nadege K. Ngombe M. Frederich Koto-te-Nyiwa Ngbolua Pius T. Mpiana Department of Chemistry Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Bila Nutraceuticals Company Dartmouth Canada Laboratory of Pharmacognosy Department of Pharmacy CIRM University of Liège CHU Liège Belgium Department of Biology Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo

The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.

关键词： Antisickling chemical fingerprint O. canum O. basilicum O. gratissimum Rosmarinic Acid

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Systematic review for geo-authentic Lonicerae Japonicae Flos

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Frontiers of Medicine 2017年第2期11卷 203-213页

作者： Xingyue Yang Yali Liu Aijuan Hou Yang Yang Xin Tian Liyun He Beijing University of Chinese Medicine Beijing 100029 China State Key Laboratory Breeding Base of Dao-di Herbs China Academy of Chinese Medical Sciences Beijing 100700 China Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences Beijing 100700 China Xiyuan Hospital China Academy of Chinese Medical Sciences Beijing 100091 China The First Clinical Medical College of Lanzhou University Lanzhou 730000 China

In traditional Chinese medicine, Lonicerae Japonicae Flos is commonly used as anti-inflammatory, antiviral, and antipyretic herbal medicine, and geo-authentic herbs are believed to present the highest quality among all samples from different regions. To discuss the current situation and trend of geo-authentic Lonicerae Japonicae Flos, we searched Chinese Biomedicine Literature Database, Chinese Journal Full-text Database, Chinese Scientific Journal Full-text Database, Cochrane Central Register of Controlled Trials, Wanfang, and PubMed. We investigated all studies up to November 2015 pertaining to quality assessment, discrimination, pharmacological effects, planting or processing, or ecological system of geo-authentic Lonicerae Japonicae Flos. Sixty-five studies mainly discussing about chemical fingerprint, component analysis, planting and processing, discrimination between varieties, ecological system, pharmacological effects, and safety were systematically reviewed. By analyzing these studies, we found that the key points of geo-authentic Lonicerae Japonicae Flos research were quality and application. Further studies should focus on improving the quality by selecting the more superior of all varieties and evaluating clinical effectiveness.

关键词： Lonicerae Japonicae Flos geo-authentic herbal quality chemical fingerprint component analysis

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HPLC fingerprint - Antioxidant Properties Study of Buckwheat

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Journal of Integrative Agriculture 2012年第7期11卷 1111-1118页

作者： ZHAO Gang PENG Lian-xin WANG Shu HU Yi-bing ZOU Liang College of Biotechnology Industly Chengdu UniversityChengdu 610106P.R.China

For quality control of buckwheat, fingerprint-efficacy study of buckwheat was carried out in this work. 2,2＇-Bipyridyl,2,2- diphenyl-l-picrylhydrazyl radical （DPPH） scavenging activity of the buckwheat was measured using UV spectrometer. chemical fingerprints of tartary buckwheat from 29 different sources were determined by HPLC-UV and investigated by similarity analysis and hierarchical clustering analysis. The fingerprint-efficacy relationship between chemical fingerprint and DPPH radical scavenging activity of tartary buckwheat were established by multi-linear regression analysis. The results showed that the sources of buckwheat had some important influence on the chemical fingerprints and DPPH radical scavenging activity. DPPH radical scavenging activity of tartary buckwheat was stronger than that of common buckwheat, rutin, and kaempferol and some unknown compound might be the major effective components for quality control of tartary buckwheat for its antioxidant activity.

关键词： buckwheat chemical fingerprint antioxidant DPPH

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Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry(DART-MS)

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Natural Products and Bioprospecting 2012年第5期2卷 206-209页

作者： Shachi SINGH Sanjay Kumar VERMA Center for Biotechnology Department of Biological SciencesBirla Institute of Technology and SciencePilani 333031India

Direct Analysis in Real Time Mass Spectrometry(DART-MS)was applied to identify and study the distribution profile of piperidine alkaloids in different parts of Prosopis juliflora,without isolation and separation of the compounds by standard chromatographic *** the help of DART-MS,chemical fingerprint of raw plant parts were generated,which revealed the presence of piperidine alkaloids in leaf,pod and flower.A comparative study of the distribution pattern,showed variation in the presence and distribution of these alkaloids in various parts of *** leaves and pod displayed the largest alkaloid pattern with a total of 12 different alkaloids in each part,whereas only 4 alkaloids were present in ***:julifloridine,prosopine,prosopinine and prosafrinine were ubiquitously distributed in all the alkaloid rich plant *** was pre-eminet alkaloid in leaf,whereas pod and flower displayed copious amounts of julifloridine.

关键词： DART-MS chemical fingerprint Prosopis juliflora piperidine alkaloids juliprosopine julifloridine

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Establishment and application of quality traceability system of traditional Chinese medicine based on two dimensional barcode using mobile intelligent technology

Establishment and application of quality traceability system...

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第一届《药学学报》药学前沿论坛暨2015年中国药学会中...

作者：李西文 CAI Yong HU Hao WANG Yi-tao CHEN Shi-lin Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of Macau

The industrial chain of traditional Chinese medicine(TCM) is a complicated process including multiple ***’s very difficult to ensure the quality to be safe,effective,stable and controllable in each link of the whole process,especially in production and circulation of Chinese materia *** almost all current traceability technologies can only carry production or circulation information without quality *** success of conversion from chemical fingerprint to two-dimensional barcode has created an opportunity to develop a quality traceability system of *** how to use this technology in circulation and consumed terminal still remains ***,this study aims to propose a new solution in order to provide a convenient way to restore the initial chemical fingerprint of *** herbal medicines were randomly selected and their chemical fingerprints were constructed and converted into 2D *** smartphone tier application will host the QR codes scanning on Android platforms that provide primary functions including restoring the initial chemical fingerprint of TCM and investigating the differences between different batches of TCM chemical *** was demonstrated that the APP can restore the initial scanning results into corresponding batches of TCM chemical *** were no significant differences between original and scanned TCM chemical ***,multiple TCM chemical fingerprint QR codes can be displayed in the same coordinate using this APP and their differences can be shown directly and intuitively.A procedure of quality traceability was also established using smartphone *** study demonstrated that smartphone can restore chemical fingerprint of TCM by scanning the QR codes,and maintain consistency with the initial chemical *** proved that chemical fingerprint of TCM can be easily generated into QR *** the QR codes can also restore the original chemical ***

关键词： quality traceability chemical fingerprint android platform application

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