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Field Assessment of the Level of Protection Conferred by a Newly Prepared Combined Inactivated Vaccine against E. coli and P. multocida in Rabbit in Egypt

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World Journal of Vaccines 2024年第2期14卷 43-56页

作者： Eman M. El Rawy Wafaa S. Abd El-Moneim Fatma M. Gad Fatma F. Ibrahim Fatma El Zahraa Gamal Abeer Mwafy Manar F. Seioudy Selim S. Salama Veterinary Serum and Vaccines Research Institute Agriculture Research Center Giza Egypt Central Laboratory for Evaluation of Veterinary Biologics Agriculture Research Center Giza Egypt Departments of Microbiology Faculty of Vet Medicine New Valley University New Valley Egypt

Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industry. Screening for bacterial agents causing mortalities in rabbits revealed the presence of Enterobacteriacae species in approximately 42% of studied cases, with E. coli the most commonly isolated organism. The present study was designed to evaluate the immune response of rabbits vaccinated with a locally prepared, combined inactivated vaccine of Pasteurella multocida and E. coli, adjuvanated with Montanide ISA70. A total of 370 rabbits, aged 2 - 3 weeks, were divided into four groups: (G1) vaccinated with a polyvalent P. multocida vaccine, (G2) vaccinated with a polyvalent E. coli vaccine, (G3) vaccinated with a combined inactivated Montanide ISA70 vaccine of P. multocida and E. coli, and (G4) kept as a non-vaccinated control group. All rabbits received two doses of 0.5 ml of the prepared vaccines, administered one month apart, and were then challenged with virulent strains of P. multocida and E. coli three weeks after the second vaccination. The prepared vaccines were evaluated by determining humoral immunity using indirect haemagglutination (IHA) test and ELISA. The potency of the vaccines was assessed through challenge and determination of LD50. Experimental findings on the prepared polyvalent combined inactivated P. multocida and E. coli vaccine indicated that it is a potent vaccine, producing the highest antibody titers and a 90% protection rate against challenges with virulent strains of P. multocida type A, D2, and E. coli types O157, O151 and O125. Thus, this vaccine is promising in addressing both P. multocida and E. coli problems in rabbits, farms, providing significant protection, and we recommend its commercial production to help rabbit producers control these two major bacterial infections.

关键词： P. multocida E. coli Vaccine ELISA challenge test Rabbit Protection

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Environmentally Safe Surfactants in Cosmetic/Detergent Formulations:Risk of Microbial Contamination and Possible Solutions

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China Detergent & Cosmetics 2017年第1期2卷 54-60页

作者： Gerardino D'Errico Department of Chemical Sciences University of Naples "Federico Ⅱ"

Eco-and bio-compatible surfactants could potentially increase the risk of microbial contamination of the formulations in which they are included. In the present work, we characterize the microstructure of an hair conditioner formulation based on the bio-compatible cationic surfactant. Behenamidopropyldimethylamine(BAPDA) and evaluated its in vitro microbiological stability. The mixture presents an isotropic micellar structure, as revealed by Electron Paramagnetic Resonance(EPR) spectroscopy. The challenge test results show that the formulation is not sel-preserving, requiring the addition of selected preservatives, whose concentration has to be carefully optimized, to inhibit the growth of contaminating micro-organisms.

关键词： cosmetic formulation surfactant preservative Electron Paramagnetic Resonance challenge test

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