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Morphology versus DNA barcoding： two sides of the same coin. A case study of ceutorhynchus erysimi and C. contractus identification

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Insect Science 2016年第4期23卷 638-648页

作者： Svetlana Stepanovic Andrea Kosovac Oliver Krstic Jelena Jovic lvo Tosevski Faculty of Science Institute of Biology and Ecology University of Kragujevac 34000 Kragujevac Serbia Department of Plant Pests Institute for Plant Protection and Environment Banatska 33 11080 Zemun Serbia CABI 1 Rue des Grillons 2800 Delemont Switzerland

Genotyping of 2 well-known weevil species from the genus ceutorhynchus （Coleoptera： Curculionidae） distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens （25 C. erysimi and 31 C. contractus） collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene （CO1）, widely used as a barcoding region, and a nuclear gene, elongation factor-v （EF-1α）, revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the CO1 and EF-1α genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for the molecular identification of 101 additional specimens of both morphospecies （33 C. erysimi and 68 C. contractus） and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.

关键词： ceutorhynchus contractus ceutorhynchus erysimi DNA barcoding molecular identification morphology PCR-RFLP

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