检索结果-南通市图书馆

Intercellular adhesion molecule-5 protects PAJU cells during in vitro ischemia following cerebral infarction

Neural Regeneration Research 2010年第3期5卷 205-208页

作者： Weijun Zhou Heping Yang Xiang Wang Yi Peng Dapeng WU Xiaojie Zhang Zhiping Hu Department of Neurology Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China Department of Neurology Ruikang Hospital Affiliated to Guangxi Traditional Chinese Medical University Nanning 530011 Guangxi Zhuang Autonomous Region China Department of Psychiatry Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China

BACKGROUND： Intercellular adhesion molecule-5 （ICAM-5） relieves the damage of beta-amyloid protein to PAJU cells, However, little is known about how ICAM-5 works as a neurotrophic factor, or whether ICAM-5 lessens neuronal damage under ischemic conditions following cerebral infarction. OBJECTIVE： To investigate the effects of ICAM-5 on PAJU cells growth in serum-free medium under ischemic conditions following cerebral infarction. DESIGN, TIME AND SETTING： The cytological in vitro study was performed at the Central Laboratory, Second Xiangya Hospital, Central South University, China, in June 2009. MATERIALS： Human ICAM-5 gene transfected into PAJU-TLN cells was supplied by the Life Science College, Helsinki University, Finland. Empty vector transfected PAJU-NEO cells were established by the Gene Center, Second Xiangya Hospital, Central South University, China. METHODS： PAJU-TLN cells transfected with human ICAM-5 or empty vector were incubated in serum-free medium. MAIN OUTCOME MEASURES： Phase contrast microscopy was used to observe changes in PAJU cell morphology. 3-（4, 5-dimethylthiazolzyl）-2, 5-diphenyltetrazolium bromide was used to determine cell viability. Hoechst 33258 was used to stain cell nuclei. Flow cytometry was utilized to measure the apoptosis rate of both PAJU-TLN and PAJU-NEO cells. RESULTS： Both PAJU-TLN and PAJU-NEO cells were injured by cultivating in serum-free medium, but the survival rate of PAJU-TLN cells was significantly higher. CONCLUSION： ICAM-5 protects PAJU-TLN cells from serum deprivation-induced apoptosis, induces the outgrowth of PAJU cells, and diminishes their morphologic impairment.

关键词： intercellular adhesion molecule-5 cerebral infarction serum deprivation neuroprotective effect brain injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Relationship between R219K polymorphism of adenosine triphosphate-binding cassette transporter 1 gene and cerebral infarction: A case-controlled analysis

引用

Neural Regeneration Research 2009年第5期4卷 396-400页

作者： Lifang Zhang Biao Chen Yanhui Du Fanyuan Kong Xianghua Fang Xiuli Feng VIP Room Affiliated Hospital of Ningxia Medical University Yinchuan 750004 Ningxia Hui Autonomous Region China Department of Neurology Beijing Xuanwu Hospital Beijing 100069 China Laboratory of Neurology Chinese National Human Genome Center Beijing 100176 China

BACKGROUND： Studies have shown that adenosine triphosphate-binding cassette transporter 1 （ABCA1） gene influences atherosclerosis. Studies have also demonstrated that cerebral infarction does not occur often in pre-menopausal women. It has been, therefore, assumed that sex plays a role in R219K polymorphism of ABCA1 gene and cerebral infarction. OBJECTIVE： To explore the relationship between lipid metabolism-correlated R219K polymorphism of ABCA1 gene, risk factors of cerebral infarction and lipid level, and to determine whether there were significant differences in gender between R219K polymorphism of ABCA1 gene and cerebral infarction. DESIGN, TIME AND SETTING： A multicentral and non-randomized, controlled study based on gene polymorphism was performed at the Chinese National Human Genome Center, and lipid concentrations were measured at Beijing Xuanwu Hospital. Patients with cerebral infarction and healthy subjects were enrolled from eight hospitals of six provinces of China between October 2002 and December 2004. PARTICIPANTS： There were 177 patients in the cerebral infarction group, including 119 males and 58 females, with a mean age of （60 -＋ 13） years, and 234 healthy subjects in the normal control group, including 79 males and 155 females, with a mean age of （58 ± 12） years. METHODS： R219K polymorphism of the ABCA1 gene was detected using polymerase chain reaction-restriction fragment length polymorphism, and blood lipid concentrations were simultaneously measured. MAIN OUTCOME MEASURES： Genotype and allele frequency of R219K polymorphic site, and blood lipid concentrations. RESULTS： RR genotype and R allele frequency of males in the cerebral infarction were significantly greater than males in the normal control group [RR genotype： x2 = 5.305, OR （95% CO, 2.326 （1.120 4.828）, P〈 0.05; R allele： x2= 4.219, OR （95% CO, 1.528 （1.019 2.292）, P〈 0.05]. In addition, RR genotype and R allele frequency of males were significantly greater than females in the cerebral

关键词： ABCA1 gene R219K polymorphism lipid cerebral infarction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plasma thrombomodulin changes in acute cerebral infarction or hypertension patients An observation for verification

引用

Neural Regeneration Research 2008年第1期3卷 71-74页

作者： Pu Feng Hui Zhang Bingyi Yang Yonggang Zheng Jinhui Xie Ying Wang Jinchuan Li Department of Internal Medicine Yuquan Hospital of Tsinghua University （Second Affiliated Hospital of Tsinghua University） Beijing100049 China

BACKGROUND： Thrombomodulin concentration greatly increases in plasma when vascular endothelial cells are injured, and it is one of the specific molecular markers for endothelial injury. OBJECTIVE： To analyze the plasma levels of thrombomodulin after cerebral infarction or hypertension, and to compare levels with those from healthy control subjects. DESIGN： A case-controlled observation. SETTING： Yuquan Hospital of Tsinghua University. PARTICIPANTS： Patients with hypertension （n = 37） and acute cerebral infarction （n = 26） were selected from the outpatient and inpatient Department of Neurology, Yuquan Hospital of Tsinghua University from February 2003 to February 2006. The cerebral infarction group consisted of 24 males and 2 females, 36-77 years of age, with a mean age of 62 years. All patients fulfilled the diagnosis criteria for cerebral infarction, according to the diagnostic standards revised by the Second National Academic Meeting for Cerebrovascular Disease, and were confirmed by CT or MRI. The hypertension group consisted of 27 males and 10 females, 36-77 years of age, with a mean age of 56 years. These patients fulfilled the diagnostic criteria for hypertension set by WHO. In addition, 43 healthy physical examinees were selected as the control group, consisting of 23 males and 20 females, 35-67 years of age. Informed consent was obtained from all participants. METHODS： In the cerebral infarction group, thrombomodulin plasma levels were determined by enzyme-linked immunoabsorbent assay at days 1, 3, 7, and 14 after attack. Thrombomodulin plasma levels were determined only once in the hypertension group and the control group. The results from the cerebral infarction group were compared with those from the hypertension group and the control group. MAIN OUTCOME MEASURES： Level of thrombomodulin in plasma. RESULTS： All 63 patients and 43 healthy volunteers were included in the final analysis of results. （1） At 7 days after the attack, the plasma levels of thrombo

关键词： thrombomudulin cerebral infarction MRI

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bilateral olfactory ensheathing cell transplantation promotes neurological function in a rat model of cerebral infarction

引用

Neural Regeneration Research 2011年第13期6卷 983-987页

作者： Zhihua Yang Wenli Sheng Huiyong Shen Qinghua Hou Rui Li Jinsheng Zeng Ruxun Huang Department of Neurology First Affiliated Hospital Jinan University Guangzhou 510120 Guangdong Province China Department of Neurology First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 Guangdong Province China Department of Orthopedics Second Affifiated Hospital Sun Yat-sen University Guangzhou 510120 Guangdong Province China

In the present study, olfactory ensheathing cells were transplanted into the cortices of infarcted （infarct transplantation group）, normal （normal transplantation group）, and bilateral hemispheres （bilateral transplantation group）. Olfactory ensheathing cells migrated to the infarct focus. The number of growth associated protein 43-positive cells and nerve fibers was slightly increased in the infarct area. These changes were more evident in the bilateral cortical transplantation group. Results demonstrated that transplanted olfactory ensheathing cells can migrate in rats with cerebra infarction. The olfactory ensheathing cells on the normal side can also promote neurological function. Bilateral cortical transplantation exhibited superior effects over unilateral transplantation.

关键词： cerebral infarction cell transplantation olfactory ensheathing cells neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Correlating plasma endothelin-1 and beta-endorphin levels to nine risk factors of acute cerebral infarction

引用

Neural Regeneration Research 2008年第2期3卷 221-224页

作者： Daoyou Zhou Jun Liu Yingrong Lao Yigang Xing Yan Huang First Department of Neurology Guangdong Hospital of Traditional Chinese Medicine Guangzhou 510120 Guangdong Province China Department of Neurology Second Affiliated Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China

BACKGROUND： Several studies have confirmed that endothelin and endorphin are involved in the occurrence of cerebral vasospasm. However, the correlation of these factors to acute cerebral infarction-related risk factors needs to be confirmed. OBJECTIVE： To detect endothelin-1 （ET-1） and beta-endorphin （β -EP） levels in plasma of patients with acute cerebral infarction, and to analyze the correlations of these factors to smoking, alcohol abuse, hypertension, diabetes mellitus, diseased region, diseased degree, gender, and other factors related to acute cerebral infarction. DESIGN： A case-control observation. SETTING： First Department of Neurology, Guangdong Hospital of Traditional Chinese Medicine; Department of Neurology, Second Affiliated Hospital of Sun Yat-sen University. PARTICIPANTS： Sixty-nine inpatients with acute cerebral infarction were admitted to the Department of Neurology, Second Affiliated Hospital of Sun Yat-sen University （March 2003-January 2004） and First Department of Neurology, Guangdong Hospital of Traditional Chinese Medicine （March July 2004） and recruited for this study. All 69 inpatients corresponded to the diagnosis criteria of acute cerebral infarction, formulated in the National Working Conference of Cerebrovascular Disease in 1998, and were confirmed as acute cerebral infarction by CT/MRI. The patient group consisted of 35 males [（644- 12） years old] and 34 females[ （674- 13 ） years old]. Among them, 9 patients were smokers, 7 were alcohol users, 48 had a history of hypertension, and 16 had a history of diabetes mellitus. CT/MRI examinations revealed that 35 patients presented with left focus sites, 11 with right ones and 23 with bilateral ones. Following attack, 24 patients had Barthel Index Scale grading 〈 40 points, 21 patients 40-50 points, and 24 patients 〉 60 points. An additional 59 healthy individuals, who received health examinations simultaneously, were included as controls. Among the control subjects, there were 37 males [（62

关键词： endothelin-1 beta-endorphin cerebral infarction factor analysis, statistical

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bilateral cerebral infarction in diabetic ketoacidosis and bilateral internal carotid artery occlusion:A case report and review of literature

引用

World Journal of Clinical Cases 2021年第15期9卷 3787-3795页

作者： Yi-Chung Chen Su-Ju Tsai Department of Physical Medicine and Rehabilitation Chung Shan Medical University HospitalTaichung 40201Taiwan

BACKGROUND Diabetic ketoacidosis(DKA)is a serious complication of type 1 diabetes mellitus(T1DM).Very rarely does DKA lead to cerebral edema,and it is even rarer for it to result in cerebral *** internal carotid artery occlusion(BICAO)is also rare and can cause fatal ***,case reports about acute cerebral infarction throughout both internal carotid arteries with simultaneous BICAO are very *** this study,we present a patient with BICAO,T1DM,hypertension,and hyperlipidemia,who had a catastrophic bilateral cerebral infarction after a DKA *** briefly introduce BICAO and the mechanisms by which DKA results in cerebral *** SUMMARY A 41-year-old woman presented with ischemic stroke that took place 3 mo prior over the left corona radiata,bilateral frontal lobe,and parietal lobe with right hemiplegia and Broca’s *** had a history of hypertension for 5 years,hyperlipidemia for 4 years,hyperthyroidism for 3 years,and T1DM for 31 *** first brain magnetic resonance imaging not only revealed the aforementioned ischemic lesions but also bilateral internal carotid artery *** was admitted to our ward for rehabilitation due to prior stroke *** took place on hospital day *** hospital day 6,she had a new massive infarction over the bilateral anterior cerebral artery and middle cerebral artery *** weeks of aggressive treatment,she remained in a coma and on mechanical ventilation due to respiratory *** discussion with her family,compassionate extubation was performed on hospital day 29 and she *** DKA can lead to cerebral infarction due to several *** people with existing BICAO and several stroke risk factors such as hypertension, T1DM,hyperlipidemia, DKA has the potential to cause more serious ischemic strokes.

关键词： Type 1 diabetes mellitus Diabetic ketoacidosis Bilateral internal carotid artery occlusion cerebral infarction Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plasma fibrinogen beta-148C/T gene polymorphism in cerebral infarction patients

引用

Neural Regeneration Research 2008年第10期3卷 1152-1155页

作者： Yuqiang Song Hongli Zou Miao Tuo Department of Neurology Affiliated Hospital of Qingdao University Medical College Qingdao 266003 Shandong Province China Department of Internal Medicine Second Affiliated Hospital of Qingdao University Medical College Qingdao 266042 Shandong Province China

BACKGROUND： Plasma fibrinogen （Fg） β-148C/T gene polymorphism is a risk factor for ischemic angiopathy. OBJECTIVE： To explore the frequency distribution of Fg β- 148C/T gene polymorphism and its relationship with plasma Fg levels in patients with cerebral infarction. DESIGN, TIME AND SETTING： Case control experiment of gene polymorphism was performed at the Central Laboratory of Qingdao University Medical College from January 2003 to June 2004. PARTICIPANTS： A total of 88 patients with cerebral infarction were recruited from the Affiliated Hospital of Qingdao University Medical College, including 52 males and 36 females, averaging （61±14） years of age In addition, 80 healthy cases served as the control group, comprising 48 males and 32 females, with an average age of （58 ± 12） years. METHODS： Blood DNA was extracted, and electrophoresis results were observed using an ultraviolet single photon image system. The frequency distribution of Fg β -148C/T was analyzed by polymerase chain reaction-restriction fragment length polymorphism method. Plasma Fg levels were measured by cerebral infarction time. MAIN OUTCOME MEASURES： Plasma Fg β -148C/T gene polymorphism and plasma Fg levels in patients with cerebral infarction. RESULTS： The frequency of the T allele, and plasma Fg levels in CC, CT, and CC＋CT genotype subgroup, were significantly greater in the cerebral infraction group, compared with the control group （P 〈 0.05）. However, there was no significant difference between the TT genotype subgroup and the control group （P 〉 0.05）. The plasma Fg levels in the CT, TT, and CT＋TT genotype groups were significantly greater than the CC genotype group （P 〈 0.05）. However, in the control group, plasma Fg levels in the TT genotype subgroup were significantly greater than the remaining genotype subgroups （P 〈 0.05）. CONCLUSION： Plasma Fg β -148C/T gene polymorphism is an important hereditary factor for differences in plasma Fg levels. The T allele plays a crucial role in influenci

关键词： cerebral infarction fibrinogen gene polymorphism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Over-expression of VEGF in Marrow Stromal Cells Promotes Angiogenesis in Rats with cerebral infarction via the Synergistic Effects of VEGF and Ang-2

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第5期32卷 724-731页

作者：赖天宝李嫚郑丽芳宋艳玲徐小丽郭远瑾张远张宗胜梅元武 Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology Zhongshan HospitalXiamen University Department of Neurology Tianyou HospitalWuhan University of Science and Technology Department of Neurology Central Hospital of Weinan City Department of Hematology Tongji Medical CollegeHuazhong University of Science and Technology

This study explored whether the transplantation of modified marrow stromal cells (MSCs) has angiogenic effects in a left middle cerebral artery occlusion infarction/reperfusion (MCAO I/R) rat model and preliminarily examined the mechanism of angiogenesis following cerebral *** were isolated by using a direct adherent method and *** endothelial growth factor (VEGF) was transfected into MSCs by employing the liposome *** transfection efficiency was measured by the optical density *** protein expression of VEGF gene before and after transfection was measured by Western *** rat model of transient occlusion of the left middle cerebral artery was established by using an approach of intra-luminal *** (TTC) and HE staining were performed to observe the cerebral *** were used to measure the levels of VEGF in the rat cerebral *** expression patterns of angiopoietin-2 (Ang-2) and CD34 in cells surrounding the area of infarction were immunohistochemistrically ***-2 protein expression in the tissue surrounding the area of infarction was measured by Western *** expression in the MSCs increased after transfection at a rate of approximately 28%±3.4%.ELISA showed that the expression of VEGF in the cerebral tissue was significantly increased after induction of infarction,peaking on the 4th day and decreasing to the levels of the sham surgery group (normal) within 7 to 10 *** VEGF level was significantly higher at each time point in the VEGF-MSC and MSC groups compared to the model ***,the VEGF level was higher in the VEGF-MSC group than in the MSC group and stayed relatively high until the 10th *** immunohistochemical results showed that 10 days after the infarction,the number of Ang-2 and CD34-expressing cells in the area surrounding the infarction was significantly higher in the VEGF-MSC group and the MSC group compared to the model ***,the

关键词： VEGF marrow stromal cells cerebral infarction angiogenesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating VEGF,SDF-1 and S100A12 expression through PI3K pathway

引用

BIOCELL 2019年第3期43卷 183-189页

作者： ZHENG LI ZHONGLE ZHAO PENG ZHANG Department of Neurosurgery People’s Hospital of Deyang CityDeyangChina Department of Emergency the Forth People’s Hospital of ShaanxiXi’anChina Department of Emergency First People’s Hospital of NantongNantongChina

Stroke remains the leading cause of death and disability worldwide,which destroys the quality of patients’lives and thus is becoming a heavy burden to the ***,the current therapeutic approaches are far from *** objective of this study is to elucidate the impact of nerve growth factor(NGF)on the brain damage induced by cerebral ischemia and its potential molecular *** cerebral artery occlusion(MCAO)rats were used as animal models and neurological functions were evaluated by modified Neurological Severity Score(NSS).Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined according to 2% 2,3,5-triphenyltetrazolium chloride(TCC)staining *** receiving NGF demonstrated significantly alleviated brain damage,reflected by a substantial improvement in the neurobehavioral outcome,a decrease in brain cell apoptosis and shrinkage of brain infarct *** analysis revealed a markedly elevated circulating vascular endothelial growth factor(VEGF)and stromal cell-derived factor 1(SDF-1)levels as well as a significant downregulation of SA10012 expression in NGF treated group compared with the untreated ***,the protective effect of NGF on cerebral ischemic injury was abolished in rats treated with both NGF and PI3K inhibitors,indicating that phosphoinositide-3-kinase(PI3K)signaling is essential for NGF *** conclusion,NGF treatment might be a potential therapeutic approach against cerebral infarction by downregulating SA10012 expression and upregulating VEGF,SDF-1 in a PI3K signaling dependent manner.

关键词： cerebral infarction Nerve growth factor PI3K SA10012 SDF-1 VEGF

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Motor Relearning Combined with t DCS on Motor Function of Lower Extremities and Gait in Patients with cerebral infarction and 3D Gait Analysis

引用

Medicinal Plant 2022年第5期13卷 74-76,82页

作者： Jing XU Su ZHENG Yuanhong XU Lili JI Chao QUAN Department of rehabilitation medicine Taihe Hospital(Hubei University of Medicine)Shiyan 442000China

[Objectives]To observe the effect of motor relearning combined with transcranial direct current stimulation on the motor function of lower extremities in patients with cerebral infarction,and to observe its effect on gait by 3D gait analysis.[Methods]60 patients with cerebral infarction who met the inclusion criteria were randomly divided into 3 groups according to the order of treatment(n=20).Group A received motor relearning treatment,group B received transcranial direct current stimulation treatment,group C received motor relearning combined with transcranial direct current stimulation,and the curative effect was observed after 5 courses of treatment.[Results]Before treatment,FMA,MBI,spatio-temporal parameters for 3D gait analysis(gait frequency,gait cycle,stride length,gait speed,stride length deviation,double support)and lower limb joint motion parameters(affected side stride length,maximum hip flexion,maximum hip extension,maximum knee flexion,maximum knee extension,stance phase,swing phase)were compared among the three *** treatment,the FMA and MBI of the three groups increased,and the spatio-temporal parameters for 3D gait analysis(gait frequency,gait cycle,gait speed,double support)and the lower limb joint motion parameters(affected side stride length,maximum hip flexion,maximum hip extension,maximum knee flexion,swing phase)were all improved,while the spatio-temporal parameters(stride length and stride length deviation)and the lower limb joint motion parameters(maximum knee extension and stance phase)*** with those before treatment,there were significant differences among the three groups(P<0.05).Through the comparison between groups,it was found that the FMA,MBI,spatio-temporal parameters for 3D gait analysis(gait frequency,gait cycle,gait speed,double support)and lower limb joint motion parameters(affected side stride length,maximum hip flexion,maximum hip extension,maximum knee flexion,swing phase)in group C were significantly hi

关键词： Motor relearning Transcranial direct current stimulation cerebral infarction Lower extremities Motor function 3D gait analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：