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CAOSA-extracted lignin improves enzymatic hydrolysis of cellulose

Green Energy & Environment 2024年第7期9卷 1101-1111页

作者： Sen Ma Zheng Li Jonathan Sperry Xing Tang Yong Sun Lu Lin Jian Liu Xianhai Zeng College of Energy State Key Laboratory of Physical Chemistry of Solid Surfaces Xiamen University Fujian Engineering and Research Centre of Clean and High-valued Technologies for Biomass Xiamen Key Laboratory of Clean and High-valued Utilization of Biomass Centre for Green Chemical Science University of Auckland

The conversion of biomass into sugar platform compounds is very important for the biorefinery industry. Pretreatment is essential to the biomass of the sugar platform, however, the lignin obtained by pretreatment, as a key part of lignocellulose, generally has a passive effect on the enzymatic hydrolysis of cellulose into sugars. In this study, p-TsOH(p-toluenesulfonic acid), DES(Deep eutectic solvent) and CAOSA(cooking with active oxygen and solid alkali) pretreatment ways were used to fraction lignin from bamboo biomass. After CAOSA treatment, the hydrolysis efficiency of the pulp was 95.57%. Moreover, the effect of different treatment methods on lignin properties was studied and the promotion effect of lignin was investigated by adding it to the cellulose enzymatic hydrolysis system. In this work, the results showed that CAOSA-extracted lignin with lower ?(1.31–1.25) had a better adsorption effect on the enzyme protein. p-TsOH-extracted lignin with a larger S/G ratio enhanced the inhibition of enzymatic hydrolysis. In addition, the presence of-COOHs in lignin could reduce its inhibitory effect on cellulose saccharification.

关键词： Biomass pretreatment CAOSA cellulose hydrolysis Lignin Enzyme

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EFFECTS OF ENDOGLUCANASE FROM Trichoderma viride A 10 IN cellulose hydrolysis

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Progress in Natural Science:Materials International 1992年第5期 449-456页

作者：高培基曲音波王祖农 Institute of Microbiology Shandong University Jinan 250100 PRC

At least 17 components of endoglucanase from Trichoderma viride A 10 have been purified to singleband in polyacrylamide gel electrophoresis by ion-exchange *** of their physical andchemical characteristics and substrate specific activities have been *** the 17 componentsare identified with the activity against glucoside linkages in amorphous cellulose and their specific activitiesfor five different types of cellulosic substrates show high *** glucanase tested can also hydrolyzecotton cellulose as associated with exo-glucan *** in combination design tests,onlynine components of endoglucanase show significant effect in hydrolysis of cotton cellulose,and furtherresearch indicates that the synergistic effect between two components of endoglucanase varied with enzymeconcentrations and one endoglucanase is enough for hydrolysis of glucoside bonds in cellulose moleculesof different cellulose substrates.

关键词： endoglucanase cellulose hydrolysis Trichoderma viride

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A boronate-modified renewable nanointerface for ultrasensitive electrochemical assay of cellulase activity

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Chinese Chemical Letters 2021年第4期32卷 1470-1474页

作者： Tianxiang Wei Qiao Xu Caiyu Zou Zeqiang He Yidan Tang Tao Gao Min Han Zhihui Dai Jiangsu Collaborative Innovation Center of Biomedical Functional Materials Jiangsu Key Laboratory of Biofunctional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China School of Environment Nanjing Normal UniversityNanjing 210023China Jiangsu Key Laboratory for Molecular and Medical Biotechnology School of Life SciencesNanjing Normal UniversityNanjing 210023China

The saccharification of cellulosic biomass to produce biofuels and chemicals is one of the most promising industries for gree n-power production and sustainable *** is the core component in the saccharification *** and efficient assay method to determine cellulase activity in saccharification is thus highly *** this work,a boronate-affinity surface based renewable and ultrasensitive electrochemical sensor for cellulase activity determination has been *** bo ronate-sugar interaction,celluloses are attached to the electrode surface,forming the cellulose na nonetwork at the sensing *** degradation can lead to the variation of electrochemical ***,electrochemical impedance signal can reflect the cellulase ***,via fully utilizing the boronate-affinity chemistry that enables reversible fabrication of cellulose nanonetwork,a renewable sensing surface has been firstly constructed for cellulase activity *** to interfacial diffusion process of electrochemical sensor,the product inhibitory effect in the cellulase activity assays can be *** proposed electrochemical sensor is ultrasensitive for label-free cellulase activity detection with a very simple fabrication process,showing great potential for activity screen of new enzymes in saccharification conversion.

关键词： Electrochemical biosensor Renewable interface Boronate-sugar interaction cellulose hydrolysis Cellulase activity

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Simultaneous Extraction of Carboxylated Celulose Nanocrystals and Nanofibrils via Citric Acid hydrolysis--A Sustainable Route

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Paper And Biomaterials 2017年第4期2卷 19-26页

作者： Chao Liu HaiShun Du Guang Yu YueDong Zhang QingShan Kong Bin Li XinDong Mu CAS Key Laboratory of Bio-based Material Qingdao Institute of Bioenergy and BioprocessTechnologyChinese Academy of SciencesQingdaoShandong Provinee266101China

In this study, cellulose nanocrystals(CNC) with surface carboxylic groups were prepared from bleached softwood pulp by hydrolysis with concentrated citric acid at concentrations of 60 wt%~80 wt%. The solid residues from acid hydrolysis were collected for producing cellulose nanofibrils(CNF) via post high-pressure homogenization. Citric acid could be easily recovered after hydrolysis reactions through crystallization due to its low water solubility or through precipitation as a calcium salt followed by acidification. Several important properties of CNC and CNF, such as dimension, crystallinity, surface chemistry, thermal stability, were evaluated. Results showed that the obtained CNC and CNF surfaces contained carboxylic acid groups that facilitated functionalization and dispersion in aqueous processing. The recyclability of citric acid and the carboxylated CNC/CNF give the renewable cellulose nanomaterial huge potential for a wide range of industrial applications. Furthermore, the resultant CNC and CNF were used as reinforcing agents to make sodium carboxymethyl cellulose(CMC) films. Both CNC and CNF showed reinforcing effects in CMC composite films. The tensile strength of CMC films increased by 54.3% and 85.7% with 10 wt% inclusion of CNC and CNF, respectively. This study provides detailed information on carboxylated nanocellulose prepared by critic acid hydrolysis; a sustainable approach for the preparation of CNC/CNF is of significant importance for their various uses.

关键词： cellulose nanocrystals cellulose nanofibrils carboxylic acid cellulose hydrolysis

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