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Photoluminescent two-dimensional SiC quantum dots for cellular imaging and transport

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Nano Research 2018年第8期11卷 4074-4081页

作者： Yu Cao Haifeng Dong Shaotao Pu Xueji Zhang Research Center for Bioengineering and Sensing Technology Beijing Key Laboratory for Bioengineering and Sensing TechnologSchool of Chemistry & Biological Engineering University of Science & Technology Beijing Beijing 100083 China

Two-dimensional （2D） ultrathin SiC has received intense attention due to its broad band gap and resistance to large mechanical deformation and external chemical corrosion. However, the synthesis and application of ultrasmall 2D SiC quantum dots （QDs） has not been explored. Herein, we synthesize a type of monolayered 2D SiC QDs with advanced photoluminescence （PL） properties via a facile hydrothermal route. Their average size and thickness can be easily adjusted by altering the reaction time. The ultrasmall 2D SiC QDs exhibit a long fluorescence lifetime of 2.59 ps due to efficient quantum confinement. The applications of SiC QDs are demonstrated through labeling A549, HeLa, and NHDF cells and delivering agents for intracellular low-abundant microRNA （miRNA） detection. This advance in preparing photoluminescent SiC QDs is of great significance for broadening their potential in biomedical and optical applications.

关键词： two-dimensional SiC quantum dots cellular imaging intraceUular microRNA(miRNA) detection

Genetically encoded X-ray cellular imaging for nanoscale protein localization

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National Science Review 2020年第7期7卷 1218-1227页

作者： Huating Kong Jichao Zhang Jiang Li Jian Wang Hyun-Joon Shin Renzhong Tai Qinglong Yan Kai Xia Jun Hu Lihua Wang Ying Zhu Chunhai Fan Bioimaging Center Shanghai Synchrotron Radiation FacilityZhangjiang Laboratory Shanghai Advanced Research Institute Chinese Academy of Sciences Division of Physical Biology CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences Canadian Light Source Inc. University of Saskatchewan Pohang Accelerator Laboratory POSTECH School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and Shanghai Key Laboratory for Nucleic Acids Chemistry and NanomedicineInstitute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University

Spatial resolution defines the physical limit of microscopes for probing biomolecular localization and interactions in cells. Whereas synchrotron-based X-ray microscopy(XRM) represents a unique approach for imaging a whole cell with nanoscale resolution due to its intrinsic nanoscale resolution and great penetration ability, existing approaches to label biomolecules rely on the use of exogenous tags that are multi-step and error-prone. Here, we repurpose engineered peroxidases as genetically encoded X-ray-sensitive tags(GXET) for site-specific labeling of protein-of-interest in mammalian cells. We find that 3,3-diaminobenzidine(DAB) polymers that are in-situ catalytically formed by fusion-expressed peroxidases are visible under XRM. Using this new tag, we imaged the protein location associated with the alteration of a DNA-methylation pathway with an ultra-high resolution of 30 nanometers. Importantly, the excellent energy resolution of XRM enables multicolor imaging using different peroxidase tags. The development of GXET enlightens the way to nanoscopic imaging for biological studies.

关键词： synchrotron-based X-ray microscopy cellular imaging nanoscale protein localization genetically encoded X-ray tags 3,3-diaminobenzidine polymers

Water-ultrastable perovskite CsPbBr_(3)nanocrystals for fluorescence-enhanced cellular imaging

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Rare Metals 2023年第5期42卷 1624-1634页

作者： Cai-Li He Zi-Qi Meng Shu-Xia Ren Jian Li Ye Wang Hao Wu Hui Bu Yu Zhang Wei-Zhong Hao Shu-Lin Chen Rong-Rong Yan Huan Liu Yu-Fang Zhu Jin-Jin Zhao Department of Neurology The Second Hospital of Hebei Medical UniversityShijiazhuang050000China Institute of Materials for Energy Conversion School of Materials Science and EngineeringShijiazhuang Tiedao UniversityShijiazhuang050043China General Practice Department Liaocheng City People’s HospitalLiaocheng252000China Tianjin Key Laboratory of Retinal Functions and Diseases Tianjin Branch of National Clinical Research Center for Ocular DiseaseEye Institute and School of OptometryTianjin Medical University Eye HospitalTianjin300000China General Practice Department Hengshui People’s HospitalHengshui053000China The 984th Hospital of the PLA Integrated TCM&Western Medicine Department Beijing100094China KAUST Catalysis Center(KCC) Division of Physical Sciences and EngineeringKing Abdullah University of Science and Technology(KAUST)Thuwal23955-6900Saudi Arabia State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Hebei Key Laboratory of Inorganic Nano-materials College of Chemistry and Materials ScienceHebei Normal UniversityShijiazhuang050024China

Metal halide perovskites have attracted much attention in biomedicine because of their excellent fluorescence energy conversion properties;however,poor water-stability and cytotoxicity limit its applications as a biomedical tracer,especially in cellular ***,water-ultrastable perovskites C sPbBr_(3):Cs_(4)PbBr_(6)nanocrystals(NCs)encapsulated in chitosan are fabricated successfully using a water-triggered *** as-synthesized CsPbBr_(3):Cs_(4)PbBr_(6)@CS(chitosan,CS)nanoparticles in water display enhanced fluorescence emission for 35 ***,the viability of glioma cells(U87 cells)incubated with different concentrations of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles(0-20μg·ml^(-1))for24 h is found to be higher than 90%.In artificial body fluid,analyses using laser confocal microscopy,the standard Cell Counting Kit-8(CCK-8)method,and flow cytometry demonstrated the good water ultrastability and high biocompatibility performance of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles in cellular ***,the water-ultrastable halide perovskites support promising perspectives in biological cell tracing and intelligent medical technology.

关键词： Perovskites nanocrystals Chitosan Waterultrastable High biocompatibility cellular imaging

Polymeric nanocomposites loaded with fluoridated hydroxyapatite Ln^(3+)(Ln = Eu or Tb)/iron oxide for magnetic targeted cellular imaging

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Cancer Biology & Medicine 2015年第3期12卷 175-183页

作者： Jie Pan Wei-Jiao Liu Chao Hua Li-Li Wang Dong Wan Jun-Bo Gong State Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Environmental and Chemical EngineeringTianjin Polytechnic University Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Science Department of Chemical Engineering School of Chemical Engineering and Technology Tianjin University

Objective： To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve specificity and sensitivity of cellular imaging under a magnetic field. Methods： Fluoridated LnS＋-doped HAP （Ln3＋-HAP） NPs and iron oxides （lOs） can be encapsulated with biocompatible polymers via a modified solvent exaction/evaporation technique to prepare polymeric nanocomposites with fluoridated Ln3＋-HAP/iron oxide. The nanocomposites were characterized for surface morphology, fluorescence spectra, magnetic properties and in vitro cytotoxicity. Magnetic targeted cellular imaging of such nanocomposites was also evaluated with confocal laser scanning microscope using A549 cells with or without magnetic field. Results： The fabricated nanocomposites showed good stability and excellent luminescent properties, as well as low in vitro cytotoxicity, indicating that the nanocomposites are suitable for biological applications. Nanocomposites under magnetic field achieved much higher cellular uptake via an energy-dependent pathway than those without magnetic field. Conclusion： 1tie nanocomposites fabricated in this study will be a promising tool for magnetic targeted cellular imaging with improved specificity and enhanced selection.

关键词： Cancer cellular imaging nanocomposites magnetic targeted hydroxyapatite (HAP) doped with rare earth

Nano-Graphene Oxide for cellular imaging and Drug Delivery

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Nano Research 2008年第3期1卷 203-212页

作者： Xiaoming Sun Zhuang Liu Kevin Welsher Joshua Tucker Robinson Andrew Goodwin Sasa Zaric Hongjie Dai Department of Chemistry and Laboratory for Advanced Materials Stanford UniversityStanfordCA 94305USA

Two-dimensional graphene offers interesting electronic,thermal,and mechanical properties that are currently being explored for advanced electronics,membranes,and *** we synthesize and explore the biological applications of nano-graphene oxide(NGO),i.e.,single-layer graphene oxide sheets down to a few nanometers in lateral *** develop functionalization chemistry in order to impart solubility and compatibility of NGO in biological *** obtain size separated pegylated NGO sheets that are soluble in buffers and serum without *** NGO sheets are found to be photoluminescent in the visible and infrared *** intrinsic photoluminescence(PL)of NGO is used for live cell imaging in the near-infrared(NIR)with little *** found that simple physisorption viaπ-stacking can be used for loading doxorubicin,a widely used cancer drug onto NGO functionalized with antibody for selective killing of cancer cells in *** to its small size,intrinsic optical properties,large specifi c surface area,low cost,and useful non-covalent interactions with aromatic drug molecules,NGO is a promising new material for biological and medical applications.

关键词： Graphene oxide pegylation size separation cellular imaging drug delivery

Facile Preparation and cellular imaging of Photoluminescent Carbogenic Nanoparticles Derived from Defoliations

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Chemical Research in Chinese Universities 2013年第1期29卷 189-192页

作者： XIU Yang ZHOU Yan GAO Qian CHEN Jie-sheng LI Guo-dong State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry National Engineering Laboratory for AIDS Vaccine College of Life Science Jilin University Changchun 130012 P. R. China" School of Chemistry and Chemical Engineering Shanghai Jiao Tong University Shanghai 200240 P. R. China

In the past two decades, photoluminescent semiconductor quantum dots（QDs） have received intensive attention for a variety of promising properties and applications, especially for their potential application in cellular imagingE. QDs with high quantum yield have been prepared based on cad mium（zinc） selenide（sulfide） and related coreshell compo sites. However, the release of Cd2＋, S2 and Se2 ions in evitably causes serious health and environmental concerns. Therefore, many efforts have been devoted to developing be nign alternatives with enhanced photoluminescence, such as silicon nanoparticles and nanowires. Carbonbased nanomaterials, including carbon nano tubes（CNTs） and carbogenic nanoparticles（CNPs）, represent an exciting new type of alternative to the aforementioned QDs with high quantum yield. CNPs are superior to QDs in chemi cally inertness, biocompatibility and photostability. After the report on the photoluminescence of carbon dots prepared by laser ablation with graphite as carbon sourcetill, several strate gies have been developed for the preparation of luminescent CNPs. For instance, luminescent CNPs were prepared by Liu et al.021 with candle soot via oxidative acid treatment, and the resulting CNPs with different emission colors were isolated by means of electrophoretic separation. Hu et al. employed an improved onestep approach for the synthesis of luminescent CNPs by laser irradiating a suspension of carbon materials in organic solvents of diamineterminated oligomeric poly（ethylene glycol）（PEG1500N）. More recently, luminescent carbogenic dots have been obtained by Peng and Travas via surface passivation of carbogenic dots in an aqueous solution with carbohydrates as the starting materials. Carbogenic nano particlesloaded magnesiumaluminophosphate solid phosphors.

关键词： Carbogenic nanoparticle Photoluminescence cellular imaging

Cytotoxicity and cellular imaging of quantum dots protected by polyelectrolyte

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Journal of Pharmaceutical Analysis 2012年第4期2卷 293-297页

作者： Hai-Yan Hu a,b,1 , Xing-Ru Dou b,1 , Zong-Lin Jiang a,Jian-Hua Tang b , Lian Xie b , Hong-Ping Xie b,n a College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, P.R. China b College of Pharmaceutical Science, Soochow University, Suzhou 215123, P.R. China College of Chemistry and Chemical Engineering China West Normal University Nanchong 637002 P.R. China. College of Pharmaceutical Science Soochow University Suzhou 215123 P.R. China.

The nanocomposites of poly-diallyldimethylammonium chloride (PDADMAC) and CdTe quantum dots (QDs) (i.e. QDs-PDADMAC nanocomposites) have been prepared based on electrostatic interaction and their fluorescence stability in aqueous solution has been investigated. MTT method (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method) was used to study their cytotoxicity and A549 lung cancer cell as a model cell was also used to evaluate their cellular imaging. It was shown that the fluorescence stability of QDsPDADMAC nanocomposites was much better than that of bare QDs both in aqueous solution and cell. Meanwhile, QDs-PDADMAC nanocomposites display very low cytotoxicity in the low concentrations and better staining ability compared with QDs. QDs-PDADMAC nanocomposites will have great advantage on the cell analysis detection and imaging.

关键词： CdTe quantum dots Poly-diallyldimethylam- monium chloride Nanocomposites Cytotoxicity cellular imaging

One-step synthesis of fluorescent smart thermo- responsive copper clusters： A potential nanothermometer in living cells

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Nano Research 2015年第6期8卷 1975-1986页

作者： Chan Wang Lin Ling Yagang Yao Qijun Song Key Laboratory of Food Colloids and Biotechnology Ministry of Education School of Chemical & Material Engineering Jiangnan University Wuxi 214122 China Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences Suzhou 215123 China State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemistry and Chemical Engineering Nanjing Tech University Nanjing 210009 China

Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） with attractive luminescent properties may be promising candidates to overcome such challenges. Here, a novel one-step synthetic method is presented to prepare highly fluorescent copper NCs （CuNCs） in ambient conditions by using glutathione （GSH） as both the reducing agent and the protective layer preventing the aggregation of the as-formed NCs. The resultant CuNCs, with an average diameter of 2.3 nm, contain 1-3 atoms and exhibit red fluorescence （A^m = 610 nm） with high quantum yields （QYs, up to 5.0%）. Interestingly, the fluorescence signal of the CuNCs is reversibly responsive to the environmental temperature in the range of 15-80 ℃. Furthermore, as the CuNCs exhibit good biocompatibility, they can pervade the MC3T3-E1 cells and enable measurements over the physiological temperature range of 15-45 ℃ with the use of the confocal fluorescence imaging method. In view of the facile synthesis method and attractive fluorescence properties, the as-prepared CuNCs may be used as photoluminescence thermometers and biosensors.

关键词： fluorescence copper nanoclusters cellular imaging nanothermometer

Emerging applications of near-infrared fluorescent metal nanoclusters for biological imaging

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Chinese Chemical Letters 2018年第10期29卷 1436-1444页

作者： Jie Xu Li Shang State Key Laboratory of Solidification Processing Center for Nano Energy MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) NPU-QMUL Joint Research Institute of Advanced Materials and Structures (JRI-AMAS) Northwestern Polytechnical University

Fluorescent metal nanoclusters（MNCs） have recently emerged as a novel kind of promising fluorescent probes for biological imaging because of their ultrasmall core size（〈2 nm）, strong photoluminescence,facile availability and good biocompatibility. In this review, we provide an update on recent advances in the development of near infrared（NIR）-emitting MNCs in terms of synthesis strategies and bioimaging applications. We mainly focus on the utilization of NIR-emitting MNCs（including Au, Ag, Cu and alloy NCs） either as single modal imaging（fluorescence intensity-based imaging, fluorescence lifetime imaging, two-photon imaging） probes or as multimodal imaging（such as NIR fluorescence/X-ray computed tomography/magnetic resonance imaging, NIR fluorescence/photoacoustic imaging/magnetic resonance imaging, NIR fluorescence/single photon emission computed tomography） probes in biological cells and tissues. Finally, we give a brief outlook on the future challenges and prospects of developing NIR-emitting MNCs for bioimaging.

关键词： Metal nanoclusters Fluorescent probes cellular imaging In vivo Near-infrared