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Salubrinal protects against tunicamycin and hypoxia induced cardiomyocyte apoptosis via the PERK-eIF2a signaling pathway

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Journal of Geriatric Cardiology 2012年第3期9卷 258-268页

作者： Chun-Lei Liu Xin Li Guo-Liang Hu Rui-Jun Li Yun-Yun He Wu Zhong Song Li Kun-Lun He Li-Li Wang Department of Cardiology Chinese PLA General Hospital28 Fuxing RoadBeijing 100853China Medical School of Nankai University 74 Weijin RoadTianjin 300074China Pharmacy Institute of Military Medical Sciences 27 Taiping RoadBeijing 100850China

Objectives This study examined the protective effect of salubrinal and the mechanism underlying this protection against tunicamycin （TM）- and hypoxia-induced apoptosis in rat cardiomyocytes. Methods Neonatal rat cardiomyocytes were cultured from the ventricles of l-day-old Wistar rats. cells were exposed to different concentrations of salubrinal （10, 20, and 40 gmol/L） for 30 min followed by TM treatment or hypoxia for 36 h. Apoptosis was measured by a multiparameter HCS （high content screening） apoptosis assay, TUNEL assay and flow cytometry. The phosphorylation of eukaryotic translation initiation factor 2 subunit alpha （eIF2c0 and the expression of cleaved caspase-12 were determined by Western blotting. C/EBP homologous protein （CHOP） was detected by immunocytochemistry. Results HCS, TUNEL assays and flow cytometry showed that salubrinal protected cardiomyocytes against apoptosis induced by TM or hypoxia. Western blotting showed that salubrinal protected cardiomyocytes against apoptosis by inducing eIF2ct phosphorylation and down-regulating the expression of the endoplasmic reticulum stress-mediated apoptotic proteins, CHOP and cleaved caspase-12. Conclusions Our study suggests that salubrinal protects rat cardiomyocytes against TM- or hypoxia-associated apoptosis via a mechanism involving the inhibition of ER stress-mediated apoptosis.

关键词： Endoplasmic reticulum stress Rat cardiomyocytes Apoptosis Salubrinal cell protection

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Pharmacological effects of icariin and icariinriside on central nervous system

Pharmacological effects of icariin and icariinriside on cent...

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2019全国老年痴呆与认知障碍相关疾病学术大会

作者： GONG Qi-hai LI Fei JIN Feng WU Qin ZHANG Feng SHI Jing-shan Key Laboratory of Basic Pharmacology of Ministry of Education/Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical University

Epimedium Brevicornum is a traditional Chinese medicinal plant possessing properties of sweet,warm,tonifying kidney,strong bones and ***,a flavonoid compound,is one of the main active ingredients of ***(ICS) is the main metabolite of *** and ICS have multiple pharmacological effects such as anti-tumor,anti-oxidative stress,improvement of cardiovascular and cerebrovascular,and regulation of *** have conducted a series of studies on the neuroprotection and mechanisms of action of icariin and ICS for many *** main findings are reported as follows.① Effect on Alzheimer disease(AD) model animals:Icariin significantly attenuated learning and memory loss,hippocampal neuron loss and senile plaque formation in APP/PS1 transgenic AD model mice,which may be related to inhibition of Aβ production and reduction of PDE5(phosphodiesterase 5).In addition,icariin significantly attenuated Aβ-induced learning and memory decline and hippocampal neuronal apoptosis in rats,which may be related to lowering PDE5 content and up-regulating BDNF/Trkb/CREB signaling pathway,inhibiting MAPK and NF-κB signaling pathways,and increasing expression of acetylcholinesterase(ACHE) and choline acetyltransferase(CHAT) in the *** the same time,icariin can significantly improve the learning and memory dysfunction induced by amanita proline in rats,which may be related to the inhibition of hippocampal neuronal apoptosis,antiexcitatory amino acid toxicity and regulation of MAPK and NF-κB signaling pathways.② Effects on Parkinson disease(PD) model animals:The study found that in LPS-induced dopaminergic neuron injury animal models and cell models,icariin can inhibit microglia by inhibiting the expression of inflammatory factors such as TNF-α,IL-1β,NO and *** of glial cells increases the expression of neurotrophic factors such as BDNF and GDNF,increases the content of dopamine(DA) and its metabolites 3,4-dihydroxyphenylaceti

关键词： icariin icariinriside Alzheimer disease Parkinson disease vascular dementia cerebral ischemia cell protection

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MRPs结合到HIT-T15胰腺β细胞：保护细胞不受四氧嘧啶的攻击(英文)

MRPs结合到HIT-T15胰腺β细胞：保护细胞不受四氧嘧啶的攻击(英文...

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华东六省一市生物化学与分子生物学会2008年学术交流会

作者：柯李晶周建武饶平凡福州大学生物工程研究所

The antidiabetic effects of the decoction of bitter melon (Momordica Charantia Linn.) pulps has been proven in vitro and in vivo by our previously studies, mainly on TypeⅡdiabetic subjects. The major active component... 详细信息

关键词： Maillard Reaction Products Bitter Melon HIT-T15 cells cell protection

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