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The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation

cell Research 2004年第5期14卷 434-438页

作者： Zhi Min YIN1,*, Jian SIMA1, Yi Fan WU1, Jian ZHU1, Yong JIANG2 1Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology, College of Life Science, Nanjing Normal University, Nanjing 210097, China 2Department of Pathophysiology and Key Lab for Shock and Microcirculation of PLA, The First Military Medical University, Guangzhou 510515, China Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology College of Life Science Nanjing Normal University Nanjing 210097 China. Zhiminy_2000@***

The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C-JNK Aa (203-424) cell line was established. After withdrawing tetracycline, the C-JNK fragment expression was induced and cell growth was dramati- cally inhibited 24 h later. However, the expresion of p53 was found to be increased after the induction of C-JNK fragment, evaluated by transfecting p21waf-luciferase reporter genes. Our further studies showed that C-JNK fragment could form complex with p53 both in vivo and in vitro. Induction of C-JNK fragment in vivo can increase p53 stability by inhibiting p53 ubiquitination.

关键词： tet-off expression system c-Jun N-terminal kinase p53 cell proliferation.

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STUDY ON PROLIFERATIVE ACTIVITY OF ENDOCRINE cellS IN GASTRIC CARCINOMA

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Chinese Journal of Cancer Research 1995年第1期7卷 48-50页

作者：虞积耀王鲁平郑集义 General Hospital of the Navy Beijing General Hospital of Beijing Army Command Beijing

Endocrine cell(EC) in 11 cases picked up from 56 cases of gastric carcinoma were observed by double immunohistochemical method using chromogramnin A(CgA) and proliferating cell nuclear antigen(PCNA).The endocrine cells were recognized by a antibody to CgA and the proliferative activity by a monoclonal antibody to PCNA.11 cases CgA positive GC cells with PCNA incorporation was in 51/4069(0-3%) with an average 1.25%.By contrast PCNA reaction was positive in 31.9±14.7% of CgA negative GC *** suggests that there were a few cell in S and G1 phase and most of them in G0 phase.

关键词： Endocrine cells Gastric carcinoma cell proliferation.

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