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Developmental nicotine exposure impair proliferation and survival of hippocampal neural cells in adolescent offspring mice

Developmental nicotine exposure impair proliferation and sur...

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中国神经科学学会第十二届全国学术会议

作者： Fei LIU Di-Qing WU Yu-Ting HU Hui YANG Xin-Rong TAO Center for Medical research Medical School Anhui University of Science and Technology Department of Biochemistry and Molecular Biology Medical School Anhui University of Science and Technology

Smoking addiction during pregnancy is still an important health problem. It associates with variety of brain impairment in the offspring including cognitive deficits, anxiety disorders and attention deficit hyperactivity disorder. The underlying mechanism of hippocampal modulation remains elusive. Objective The purpose of this study is to investigate the proliferation and survival of hippocampal neural cells in mouse offspring of nicotine exposure during pre-pregnancy, pregnancy and breastfeeding. Methods 7-weeks-old C57 BL/6 female mice were administrated with nicotine（200 μg/ml in 2% saccharin） via drinking water starting from 2 weeks pre-mating until the offspring were weaned, while control female mice were received 2% saccharin. Locomoter activity and anxiety-like behavior were analyzed by open field test in adolescent offspring（P35）. 5-Bromo-2-deoxyuridine（Brd U） intraperitoneal injection was used to detect the proliferation and survival of neural cells in hippocampus. Immunofluorescence staining was performed to colocalize Brd U/Neu N, Brd U/GFAP or Brd U/Iba-1 double positive neural cells. Results（1） Total distance and immobility within one hour showed no significant difference in the open field between nicotine and control group. However, the exploration time in the center of the open field reduced remarkly in both male and female adolescent offspring mice of nicotine group compared to the control（P ＜ 0.05）.（2） Both male and female mice in nicotine offspring group showed less newborn neurons relative to the control（P ＜ 0.05）.（3） We found that offspring male mice in nicotine group had more Brd U positive neurons（Neu N+/Brd U+） in the dentate gyrus compared to the female ones although no statistic difference was detected. Surprisingly, nicotine offspring male mice exhibited more Brd U positive（GFAP+/Brd U+ and Iba1+/Brd U+） neuroglia in CA1 and CA2 region than nicotine female ones. Conclusion These results suggest that developmental nicotine exposure contribut

关键词： Nicotine neurotoxicity Neural development Hippocampal neural cells cell proliferation and survival Children’s disorders Prenatal exposure Animal model

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