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第三届全国微生物资源学术暨国家微生物资源平台运行服务研讨会

作者： Yanchun Yan,Jie Qu,Haisheng Wang,Wei Wu Graduate School,Chinese Academy of Agricultural Sciences

Two strains,L31 and L13,capable of degrading and utilizing cypermethrin as the sole carbon and energy sources for growth in the minimal salt medium(MSM),were isolated from a pesticide industry’s *** L31 and L13 were identified as Exiguobacterium acetylicum L31 and Klebsiella pneumoniae L13 based on the morphology,physio-biochemical characteristics and 16S rRNA *** L31 could degrade 83% of cypermethrin(50mg/L) within 5 days and the degradation rate of strain L13 reached 66% within 5 days,indicating that L31 has higher degrading ability than strain *** of them could transform cypermethrin to 3-phenoxybenzoic acid(3-PBA).Results of microbial adherence to hydrocarbons(MATH) and biodegradation assays illuminated that strain L31 has higher hydrophobicity and degrading ability than strain L13,suggesting that the high hydrophobicity of strains could enhance the degradation of cypermethrin.

关键词： Cypermethrin biodegradation Exiguobacterium acetylicum L31 cell surface hydrophobicity

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<i>In Vitro</i>Characterization of cell surface Properties of 14 Vaginal <i>Lactobacillus</i>Strains as Potential Probiotics

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Advances in Microbiology 2021年第2期11卷 144-155页

作者： Shao-Ji Li Jae-Seong So SignalDT Biotechnologies (SZ) Inc. Shenzhen China Department of Biological Engineering Inha University Incheon Korea

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Human-origin Lactobacillus is a preferable source of probiotic bacteria. This study screened 14 vaginal Lactobacillus strains as probiotic candidates by investigating probiotic-related cell surface characteristics including cell surface hydrophobicity (CSH), Lewis acidity/basicity, autoaggregation, and biofilm formation. Moderate to high CSH and autoaggregation, high basicity and low acidity were prevalent in the 14 tested strains. Biofilm formation varied in a large range among the 14 tested strains. CSH showed a high correlation with Lewis acidity and autoaggregation, while Lewis acidity was highly correlated with autoaggregation and biofilm formation. Four strains were selected as promising probiotic strains. This study was the first one to compare antibiotic sensitivity between biofilm-forming cells and planktonic cells of Lactobacillus species, and found that biofilm-forming cells of a L. fermentum strain had a significantly higher survival rate than planktonic cells in cefotaxime, cefmetazole and tetracycline, but were as sensitive to oxacillin and ampicillin as planktonic cells were.

关键词： Lactobacillus Probiotics cell surface hydrophobicity Lewis Acidity Lewis Basicity Autoaggregation Biofilm Antibiotics

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