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IJLAI Transactions on Science and Engineering 2024年第3期2卷 66-71页

作者： Mirza Abdul Aleem Baig Center for Biomedical Engineering School of Information Science and Technology University of Science and Technology of China

Context:Bioinspired Artificial Intelligence(Bio-AI)has emerged as a transformative tool in biomedical research,addressing challenges in cell labeling essential for understanding cellular behavior and *** cell labeling methods often struggle with accuracy,scalability,and adaptability in complex ***:This paper theoretically explores the integration of Bio-AI models into cell labeling *** aims to assess how these models C encompassing neural networks,swarm intelligence,evolutionary algorithms,and self-organizing maps C can enhance cell identification and ***:The paper examines various Bio-AI models that mimic biological processes such as neural functioning,swarm behavior,and evolutionary *** also evaluates the application of multimodal AI systems that combine imaging data with molecular and genetic *** addition,the potential of dynamic cell labeling,inspired by neural plasticity,is ***:The integration of Bio-AI models has demonstrated significant improvements in accuracy,adaptability,and scalability for cell *** labeling systems minimize human error and enhance *** advancements in multimodal AI systems have shown promise in combining imaging with genetic and molecular data,providing more comprehensive insights into cellular *** labeling models inspired by neural plasticity offer enhanced tracking of cellular transitions over ***:Bio-AI holds transformative potential in biomedical research via enabling real-time,dynamic labeling,essential for tracking cellular changes over time in processes like cancer progression,stem cell differentiation,and immune *** continued evolution of these AI-driven approaches is expected to accelerate breakthroughs in understanding diseases,tissue engineering,and regenerative medicine.

关键词： Bioinspired Artificial Intelligence Biomedical Research cell labeling Bio-AI Models

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Artificial light-harvesting systems and their applications in photocatalysis and cell labeling

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ChemPhysMater 2022年第4期1卷 281-293页

作者： Ying Wang Ning Han Xinglong Li Shengsheng Yu Lingbao Xing School of Chemistry and Chemical Engineering Shandong University of TechnologyZibo 255000China Department of Materials Engineering KU LeuvenLeuven 3001Belgium

Photosynthesis is the basis for the survival of organisms in nature;consequently,the fabrication of artificial light-harvesting systems(LHSs)that simulate natural photosynthesis is of significant ***,a variety of artificial LHSs have been successfully constructed using fluorescence resonance energy transfer(FRET).However,it is crucial to fabricate artificial LHSs with a sequential energy transfer process when considering that the natural photosynthetic process involves a multistep sequential energy transfer process rather than a simple one-step energy ***,many previously reported LHSs have been used as imaging agents for cell labeling and bioimaging or as catalysts in photocatalytic reactions,showing promise for applications simulating natural *** this review,we have summarized recently published representative work on artificial *** addition,the application of LHSs in photocatalysis and cell labeling has been described in detail.

关键词： Light-harvesting systems Sequential energy transfer Photocatalysis cell labeling

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Contrast Agents and cell labeling Strategies for in Vivo Imaging

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Advances in Nanoparticles 2014年第2期3卷 41-53页

作者： Marta Legacz Katharina Roepke Michael Giersig Ulrich Pison Department of Anaesthesiology Charite-Universitatsmedizin BerlinCampus Virchow-KlinikumBerlinGermany TOPASS GmbH BerlinGermany Department of Physics Freie Universitat BerlinBerlinGermany

Regenerative medicine has become a new therapeutic approach in which stem cells or genetically reprogrammed cells are delivered to diseased areas in the body with the intention that such multipotent cells will differentiate into healthy tissue and exchange damaged tissue. The success of such cell-based therapeutic approaches depends on precise dosing and delivery of the cells to the desired site in the human body. To determine the accuracy and efficacy of the therapy, tracking of the engrafted cells in an intact living organism is crucial. There is a great need for sensitive, noninvasive imaging methods, which would allow clinicians to monitor viability, migration dynamics, differentiation towards specific cell type, regeneration potential and integration of transplanted cells with host tissues for an optimal time period. Various in vivo tracking methods are currently used including: MRI (Magnetic Resonance Imaging), PET (Positron Emission Tomography), SPECT (Single Photon Emission Computer Tomography), optical imaging (OI), photoacoustic imaging (PAI) and ultrasound (US). In order to carry out the detection with each of the aforementioned techniques, the cells must be labeled either exogenously (ex vivo) or endogenously (in vivo). For tracking the administrated cells, scientists usually manipulate cells outside the living organism by incorporating imaging contrast agents (CAs) or reporter genes. Strategies for stem cell labeling using CAs will be reviewed in the light of various imaging techniques.

关键词： In Vivo Imaging MRI PET SPECT SPIO cell labeling

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Covalent stabilization of DNA nanostructures on cell membranes for efficient surface receptor-mediated labeling and function regulations

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Science China Chemistry 2022年第11期65卷 2327-2334页

作者： Dandan Chao Xuemei Xu Yanyan Miao Linlin Yang Qianqian Gao Rui Xu Yuan Tian Yumeng Zhao Yuzhen Du Da Han Institute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and NanomedicineState Key Laboratory of Oncogenes and Related GenesRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Laboratory Medicine Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200127China

One major challenge of using DNA nanostructures for cellular and in vivo applications is their insufficiently structural integrity that stems from the non-covalent base pairing and stacking in complex cellular and physiological environment. The establishment of covalent bonds in DNA nanostructures can link individual strands more stably and therefore should improve the performance of DNA nanostructures in different scenarios where structural integrity is required. Here, we developed a convenient and effective method for constructing covalently stabilized DNA nanostructures by chemically inserting photo-crosslinker(^(CNV)K) in DNA sequences. These covalently linked DNA nanostructures were found to be more resistant to external interference, such as low cation concentrations and unspecific displacement on cell membranes. We also demonstrated that our strategy could improve the efficiency of cell surface receptor-mediated labeling and function regulations in living cells, which sheds light on broadening the biomedical applications of DNA nanostructures.

关键词： DNA nanostructures covalent stabilization structural integrity cell labeling surface receptor regulation

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Spy chemistry enables stable protein immobilization on iron oxide nanoparticles with enhanced magnetic properties

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Journal of Materials Science & Technology 2023年第30期161卷 161-169页

作者： Chu Shi Jingyi Sheng Guangxiang Si Yan Li Yan Shen Ning Gu Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210009China School of Biomedical Engineering and Informatics Nanjing Medical UniversityNanjing 211166China Medical School Nanjing UniversityNanjing 210093China

Iron oxide nanoparticles(IONPs)modified with functional proteins hold great promise in the biomedical ***,conventional protein modification strategies,such as adsorption and covalent coupling,are either unstable or nonspecific,or may result in the changes of protein structure and ultimately the loss of protein *** of active proteins on small-sized IONPs with a particle size of less than 30 nm is especially difficult due to their high surface ***,we developed a universal modifica-tion method based on Spy chemistry for rapid and stable protein immobilization on small-sized IONPs,which only requires the presence of active groups on the surface of nanoparticles that can couple with *** short,the SpyCatcher peptides were first coated on the surface of IONPs by cross-linking with activated groups,and then the SpyTag peptide fused with a model protein(enhanced green fluo-rescent protein,EGFP)was engineered(SpyTag-EGFP)and directly coupled to SpyCatcher-modified IONPs by self-assembly,which is spontaneous and robust while avoiding the effect of chemical reactions on functional protein *** obtained EGFP-functionalized IONPs exhibited enhanced and stable green fluorescence and improved magnetic *** addition,the cell internalization efficiency of EGFP-functionalized IONPs was significantly increased as compared to unmodified IONPs,providing an ideal solution for efficient cell labeling and *** conclusion,here we report a rapid and easy strategy for EGFP immobilization on IONPs based on Spy chemistry,which could be further adapted to other functional proteins in the ***-modified IONPs and SpyTag-X(arbitrary functional fusion proteins)hold great potential to be applied as a versatile platform for protein immobilization on IONPs and enable its multifunctional application in the future.

关键词： Iron oxide nanoparticles Spy chemistry Protein immobilization Magnetic resonance imaging cell labeling

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Folate-conjugated Fe_3O_4 nanoparticles for in vivo tumor labeling

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Transactions of Nonferrous Metals Society of China 2013年第7期23卷 2079-2084页

作者：周春姣王少华周宇容鹏飞陈孜孜刘进言周建大湖南农业大学理学院长沙410128 中南大学湘雅三医院长沙410013

Highly biocompatible superparamagnetic Fe3O4 nanoparticles were synthesized by amide of folic acid （FA） ligands and the NH2-group onto the surface of Fe3O4 nanoparticles. The as-synthesized folate-conjugated Fe3O4 nanoparticles were characterized by X-ray diffraction diffractometer, transmission electron microscope, FT-IR spectrometer, vibrating sample magnetometer, and dynamic light scattering instrument. The in vivo labeling effect of folate-conjugated Fe3O4 nanoparticles on the hepatoma cells was investigated in tumor-bearing rat. The results demonstrate that the as-prepared nanoparticles have cubic structure of Fe3O4 with a particle size of about 8 nm and hydrated diameter of 25.7 nm at a saturation magnetization of 51 A·m2/kg. These nanoparticles possess good physiological stability, low cytotoxicity on human skin fibroblasts and negligible effect on Wistar rats at the concentration as high as 3 mg/kg body mass. The folate-conjugated Fe3O4 nanoparticles could be effectively mediated into the human hepatoma Bel 7402 cells through the binding of folate and folic acid receptor, enhancing the signal contrast of tumor tissue and surrounding normal tissue in MRI imaging. It is in favor of the tumor cells labeling, tracing, magnetic resonance imaging （MRI） target detection and magnetic hyperthermia.

关键词： Fe3O4 nanoparticles superparamagnetic property folic acid tumor conjugation magnetic resonance imaging (MRI) cell labeling

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Magnet-targeted delivery of bone marrow-derived mesenchymal stem cells improves therapeutic efficacy following hypoxic-ischemic brain injury

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Neural Regeneration Research 2021年第11期16卷 2324-2329页

作者： Chuang Sun Ao-Dan Zhang Hong-Hai Chen Jie Bian Zheng-Juan Liu Department of Radiology The Second Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Department of Pediatrics The Second Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina

hypoxicischemic brain injury;however,the therapeutic efficacy of bone marrow-derived mesenchymal stem cells largely depends on the number of cells that are successfully transferred to the ***-targeted drug delivery systems can use a specific magnetic field to attract the drug to the target site,increasing the drug *** this study,we found that the double-labeling using superparamagnetic iron oxide nanoparticle and poly-L-lysine(SPIO-PLL)of bone marrow-derived mesenchymal stem cells had no effect on cell survival but decreased cell proliferation 48 hours after *** models of hypoxic-ischemic brain injury were established by ligating the left common carotid *** day after modeling,intraventricular and caudal vein injections of 1×105 SPIO-PLL-labeled bone marrow-derived mesenchymal stem cells were ***-four hours after the intraventricular injection,magnets were fixed to the left side of the rats’heads for 2 *** incoherent motion magnetic resonance imaging revealed that the perfusion fraction and the diffusion coefficient of rat brain tissue were significantly increased in rats treated with SPIO-PLL-labeled cells through intraventricular injection combined with magnetic guidance,compared with those treated with SPIO-PLL-labeled cells through intraventricular or tail vein injections without magnetic ***-eosin and terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL)staining revealed that in rats treated with SPIO-PLL-labeled cells through intraventricular injection under magnetic guidance,cerebral edema was alleviated,and apoptosis was *** findings suggest that targeted magnetic guidance can be used to improve the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for hypoxic-ischemic brain *** study was approved by the Animal Care and Use Committee of The Second Hospital of Dalian Medical University,China(approval No.2016-060)on

关键词： bone marrow-derived mesenchymal stem cells cell apoptosis diffusion coefficient cell labeling intraventricular injection intravoxel incoherent motion magnetic guidance perfusion fraction superparamagnetic nanoparticles

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Magnetic resonance evaluation of human mesenchymal stem cells in corpus cavernosa of rats and rabbits

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Asian Journal of Andrology 2007年第3期9卷 361-367页

作者： Yun-Seob Song Ja-Hyeon Ku Eun-Seop Song Jung-Hoon Kim Jin-Suck Jeon Kong-Hee Lee Sook-Ja Kim Hee-Jeong Cheong Ik-Sung Lim Dongho Choi Jong-Ho Won Department of Urology Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Urology Seoul National University Hospital Seoul 110-744 Korea Department of Obstetrics and Gynecology Inha University School of Medicine Incheon 400-103 Korea Department of Radiology Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Internal Medicine Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Urology Soonchunhyang School of Medicine Seoul 140-743 Korea Stem cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Industrial Management ＆ Engineering Namseoul University College of Engineering Cheonan 330-707 Korea Department of Surgery Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Internal Medicine Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea

Aim： To investigate whether the biological process of superparamagnetic iron oxide （SPIO）-labeled human mesenchymal stem cells （hMSCs） may be monitored non-invasively by using in vivo magnetic resonance （MR） imaging with conventional 1.5-T system examinations in corpus cavernosa of rats and rabbits. Methods： The labeling efficiency and viability of SP10-labeled hMSCs were examined with Prussian blue and Tripan blue, respectively. After SPIO-labeled hMSCs were transplanted to the corpus cavernosa of rats and rabbits, serial T2-weighted MR images were taken and histological examinations were carried out over a 4-week period. Results： hMSCs loaded with SPIO compared to unlabeled cells had a similar viability. For SPIO-labeled hMSCs more than lx 105 concentration in vitro, MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in the rat and rabbit corpus cavernosa decreased and was confined locally. After injection of SPIO-labeled hMSCs into the corpus cavernosum, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks after injection. The presence of iron was confirmed with Prussian blue staining in histological sections. Conclusion： SP10-labeled hMSCs in corpus cavernosa of rats and rabbits can be evaluated non-invasively by molecular MR imaging. Our findings suggest that MR imaging has the ability to test the long-term therapeutic potential of hMSCs in animals in the setting of erectile dysfunction.

关键词： human mesenchymal stem cells magnetic resonance stem cells penis cell labeling corpus cavemosa

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Cobalt protoporphyrin-induced nano-self-assembly for CT imaging,magnetic-guidance,and antioxidative protection of stem cells in pulmonary fibrosis treatment

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Bioactive Materials 2023年第3期21卷 129-141页

作者： Yimeng Shu Ming Ma Xiaoxia Pan Muhammad Shafiq Huizhu Yu Hangrong Chen State Key Laboratory of High Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai200050PR China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049PR China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences1 Sub-laneXiangshanHangzhou310024PR China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai200438PR China Department of Biotechnology Faculty of Science and TechnologyUniversity of Central Punjab(UCP)Lahore54000Pakistan

Mesenchymal stem cells(MSCs)transplantation is a promising approach for pulmonary fibrosis(PF),however it is impeded by several persistent challenges,including the lack of long-term tracking,low retention,and poor survival of MSCs,as well as the low labeling efficiency of ***,a cobalt protoporphyrin IX(CoPP)aggregation-induced strategy is applied to develop a multifunctional nano-self-assembly(ASCP)by combining gold nanoparticle(AuNPs),superparamagnetic iron oxide nanoparticles(SPIONs),and CoPP through a facile solvent evaporation-driven *** no additional carrier materials are employed during the synthesis,high loading efficiency of active ingredients and excellent biocompatibility are ***,facile modification of the ASCPs with bicyclo[6.1.0]nonyne(BCN)groups(named as ASCP-BCN)enables them to effectively label MSCs through bioorthogonal *** obtained ASCP-BCN could not only help to track MSCs with AuNP-based computed tomography(CT)imaging,but also achieve an SPIONs-assisted magnetic field based improvement in the MSCs retention in lungs as well as promoted the survival of MSCs via the sustained release of *** in vivo results demonstrated that the labeled MSCs improved the lung functions and alle-viated the fibrosis symptoms in a bleomycin–induced PF mouse ***,a novel ASCP-BCN multi-functional nanoagent was developed to bioorthogonally-label MSCs with a high efficiency,presenting a promising potential in the high-efficient MSC therapy for PF.

关键词： Self-assembly Pulmonary fibrosis cell labeling Stem cell therapy Theranostic

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Superparamagnetic Iron Oxide labeling of Neural Stem cells and 4.7T MRI Tracking in vivo and in vitro

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第1期27卷 107-110页

作者：朱文珍李祥唐洲平朱遂强漆剑频魏黎雷浩 Department of Radiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

Neural stem cells were labeled with superparamagnetic iron oxide （SPIO） and tracked by MRI in vitro and in vivo after implantation, Rat neural stem cells were labeled with SPIO combined with PLL by the means of receptor-mediated endocytosis. Prussian blue staining and electron microscopy were conducted to identify the iron particles in these neural stem cells. SPIO-labeled cells were tracked by 4.7T MRI in vivo and in vitro after implantation, The subjects were divided into 5 groups, including 5× 10^5 labeled cells cultured for one day after labeling, 5 × 10^5 same phase unlabeled cells, cell culture medium with 25μg Fe/mL SPIO, cell culture medium without SPIO and distilled water. MR/scanning sequences included TIWI, T2WI and T2＊WI. R2 and R2＊ of labeled cells were calculated. The results showed：（1） Neural stem cells could be labeled with SPIO and labeling efficiency was 100%. Prussian blue staining showed numerous blue-stained iron particles in the cytoplasm; （2） The average percentage change of signal intensity of labeled cells on TIWI in 4.7T MRI was 24.06%, T2WI 50.66% and T2＊WI 53.70% respectively; （3） T2 of labeled cells and unlabeled cells in 4.7T MRI was 516 ms and 77 ms respectively, R2 was 1.94 s^-1 and 12.98 s^-1 respectively, and T2＊ was 109 ms and 22.9 ms, R2＊ was 9.17 s^-1 and 43.67 s^-1 respectively; （4） Remarkable low signal area on T2WI and T2＊WI could exist for nearly 7 weeks and then disappeared gradually in the left brain transplanted with labeled cells, however no signal change in the right brain implanted with unlabeled cells. It was concluded that neural stem cells could be labeled effectively with SPIO. R2 and R2＊ of labeled cells were increased obviously. MRI can be used to track labeled cells in vitro and in vivo.

关键词： stem cells cell labeling magnetic resonance imaging animals

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