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Transplantation of fetal liver epithelial progenitor cells ameliorates experimental liver fibrosis in mice

World Journal of Gastroenterology 2006年第45期12卷 7292-7298页

作者： Jin-Fang Zheng Li-Jian Liang Chang-Xiong Wu Jin-Song Chen Zhen-Sheng Zhang Department of Hepatobiliary Surgery the First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Department of Hepatobiliary Surgery the People's Hospital of Hainan Province Haikou 570311 Hainan Province China

AIM： To investigate the effect of transplanted fetal liver epithelial progenitor （FLEP） cells on liver fibrosis in mice. METHODS： FLEP cells were isolated from embryonal day （ED） 14 BALB/c mice and transplanted into female syngenic BALB/c mice （n = 60）. After partial hepatectomy （PH）, diethylnitrosamine （DEN） was administered to induce liver fibrosis. Controls received FLEP cells and non-supplemented drinking water, the model group received DEN-spiked water, and the experimental group received FLEP cells and DEN. Mice were killed after 1, 2, and 3 mo, and alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, hyaluronic acid （HA）, and laminin （LN） in serum, and hydroxyproline （Hyp） content in liver were assessed. Alpha-smooth muscle actin （α-SMA） of liver was tested by immunohistochemistry. Transplanted male mice FLEP cells were identified by immunocytochemistry for sty （sex determination region for Y chromosome） protein. RESULTS： Serum ALT, AST, HA, and LN were markedly reduced by transplanted FLEP cells. Liver Hyp content and （α-SMA staining in mice receiving FLEP cells were lower than that of the model group, which was consistent with altered liver pathology. Transplanted cells proliferated and differentiated into hepatocytes and bile duct epithelial cells with 30%-50% repopulation in the liver fibrosis induced by DEN after 3 mo. CONCLUSION： Transplanted FLEP cells proliferate and differentiate into hepatocytes and bile duct epithelial cells with high repopulation capacity in the fiberized liver induced by DEN, which restores liver function and reduces liver fibrosis.

关键词： Fetal liver Epithelial progenitor cells ce therapy Proliferation Liver fibrosis

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