检索结果-南通市图书馆

Efficient generation of targeted point mutations in the Brassica oleracea var.botrytis genome via a modified CRISPR/Cas9 system

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2022年第4期8卷 527-530页

作者： Guixiang Wang Mei Zong Di Liu Yage Wu Shouwei Tian Shuo Han Ning Guo Mengmeng Duan Liming Miao Fan Liu Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Ministry of AgricultureBeijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China

In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants.For ALS gene,a C-to-T conversion in the Pro182 codon(CCT)can alter the encoded amino acid,likely resulting in herbicide resistance,and a C-to-T mutation in the Leu133 codon(CTT)in the CENH3 gene may produce a haploid inducer.Results indicated that the transformation efficiency was 1.8%–4.5%and the mutation efficiencies for the ALS and CENH3 genes were approximately 22%and 87%,respectively.The ALS mutant cauliflower showed strong herbicide resistance,with possible immediate implications for broadleaf weed control in cauliflower fields.

关键词： cauliflower Targeted point mutations Base-editing CRISPR/Cas9 ALS CENH3

Draft genome sequence of cauliflower(Brassica oleracea L.var.botrytis)provides new insights into the C genome in Brassica species

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 740-750页

作者： Deling Sun Chunguo Wang Xiaoli Zhang Wenlin Zhang Hanmin Jiang Xingwei Yao Lili Liu Zhenghua Wen Guobao Niu Xiaozheng Shan Tianjin Academy of Agricultural Sciences 300192 TianjinChina College of Life Sciences Nankai University300071 TianjinChina Tianjin Kernel Vegetable Research Institute 300384 TianjinChina Novogene Bioinformatics Institute 100015 BeijingChina

cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig N50 of 2.11 Mb,and contained 47,772 genes;56.65%of the genome was composed of repetitive sequences.Among these sequences,long terminal repeats(LTRs)were the most abundant(32.71%of the genome),followed by transposable elements(TEs)(12.62%).Comparative genomic analysis confirmed that after an ancient paleohexaploidy(γ)event,cauliflower underwent two whole-genome duplication(WGD)events shared with Arabidopsis and an additional whole-genome triplication(WGT)event shared with other Brassica species.The present cultivated cauliflower diverged from the ancestral B.oleracea species~3.0 million years ago(Mya).The speciation of cauliflower(~2.0 Mya)was later than that of B.oleracea L.var.capitata(approximately 2.6 Mya)and other Brassica species(over 2.0 Mya).Chromosome no.03 of cauliflower shared the most syntenic blocks with the A,B,and C genomes of Brassica species and its eight other chromosomes,implying that chromosome no.03 might be the most ancient one in the cauliflower genome,which was consistent with the chromosome being inherited from the common ancestor of Brassica species.In addition,2,718 specific genes,228 expanded genes,2 contracted genes,and 1,065 positively selected genes in cauliflower were identified and functionally annotated.These findings provide new insights into the genomic diversity of Brassica species and serve as a valuable reference for molecular breeding of cauliflower.

关键词： species cauliflower sequence

Residue patterns of indoxacarb and pyridalyl in treated cauliflower

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences 2013年第3期4卷 111-116页

作者： Ji-Young Yoon Jae-Hun Park Hye-Ree Moon Guk-Tak Han Kyu-Seung Lee Department of Bio-Environmental Chemistry Chungnam National University Daejeon Republic of Korea National Agriculture Product Quality Management Service Chungnam Division Branch Office Daejeon Republic of Korea

The biological half-life and final residue levels of indoxacarb and pyridalyl were determined in cauliflower over a 10-day cultivation period following applications of a standard (100 g a.***.ha–1 and 200 g a.i.·ha–1, respectively) and double dose (200 g a.i.·ha–1 and 400 g a.i.·ha–1, respectively). The residue levels were analyzed by gas chromatography with recovery ranging from 92.1% to 109.7%. The biological half-lives of indoxacarb and pyridalyl were 6.33 and 7.74 days for the standard dose, and 6.26 and 7.44 days for the double dose, respectively. The initial and persisting concentrations of indoxacarb and pyridalyl were all below the Korean maximum residue limits for broccoli of 1.0 mgkg–1 and 3.0 mgkg–1, respectively.

关键词： Biological Half-Life cauliflower Indoxacarb Pyridalyl Residue

Persistence of Indoxacarb on cauliflower (Brassica oleracea var. botrytis.L.) and Its Risk Assessment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Analytical Chemistry 2011年第8期2卷 69-76页

作者： Reenu Takkar S. K. Sahoo Gurmail Singh Kousik Mandal R. S. Battu Balwinder Singh 不详

A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recoveries at four different spiking concentrations of 0.01, 0.05, 0.1 and 0.2 mg kg–1 ranged from 87 to 96% were achieved with good repeatability and RSD of 1% - 6%. The average initial deposits of 0.23 and 0.45 mg kg–1 were observed after last application of indoxacarb @ 52.2 and 104.4 g. a.i. ha–1 at recommended and double the recommended dosages, respectively. The residues in cauliflower dissipated below its LOQ of 0.01 mg kg–1 after 7 days and its half-life periods were observed to be 1.12 and 1.31 days, respectively, at single and double the dosages. Keeping in view 80 g consumption of cauliflower curds per day for a 55 kg person, theoretical maximum residue contribution (TMRC) of indoxacarb when calculated from maximum residues observed on 0 day samples at recommended and double the recommended dosages, respectively, were found to be 20.8 and 36.8 μg in comparison to its acceptable daily intake (ADI) of 550 μg, which is quite safe.

关键词： cauliflower Indoxacarb MRL QuEChERS Residue

Developmental Response of Pieris brassicae(L.) (Lepidoptera: Pieridae) on Different cauliflower Cultivars under Laboratory Conditions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2014年第18期5卷 2611-2616页

作者： Amna Sadozai Imtiaz Ali Khan Entomology Department Faculty of Crop Protection The University of Agriculture Peshawar Pakistan

cauliflower is popular vegetable in Pakistan and it is severely attacked by Pieris brassicae (L). There are different cauliflower cultivars available in this area. The aim of this study was to determine the developmental response of Pieris brassicae on ten cauliflower cultivars. An experiment was conducted during 2012-13 at the Entomology section of the Agriculture Research Institute Tarnab Peshawar under laboratory conditions of 20°C ± 2°C, 50% ± 5% RH and 12:12h photoperiod. Pieris brassicae eggs were collected from a cauliflower field ARI Tarnab and the larvae placed after hatching on fresh leaves often cauliflower cultivars. The results showed that the larval development of P. brassicae was short (35 days), longer larval length (32.41 mm), larval mortality was low (6.6%) and pupal weight was high (0.50 g) on Clima cultivar. On the other hand, a longer larval developmental period (37 days), higher larval mortality (86.66%), shorter larval length (24.55 mm) and lower pupal weight (0.42 g) were recorded on cultivar AX-2034.

关键词： Pieris brassicae Biology cauliflower

Control Effects of Ten Kinds of Insecticides on Main Lepidopteran Pests of cauliflower

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Diseases and Pests 2019年第4期10卷 34-35,42页

作者： Ma Jiang Yun Qingyuan Institute of Agricultural Environment and Sustainable Development Chinese Academy of Agricultural SciencesBeijing 100081China Wenchang Street Office Demonstration Area of Urban-Rural Integration of JiaozuoJiaozuo 454350China

[Objective]The paper was to screen out effective insecticides against main lepidopteran pests of cauliflower.[Method]Field trials were carried out in 2018,and 11 treatments were designed in the test.The population decline rate and corrected control effect were calculated.[Result]16%Indoxacarb+tebufenozide EC and 35%methoxyfenozide+indoxacarb SC had quick bioactivity and persistence,and the control effects at 1 and 10 d post spraying were greater than 85%.11%Emamectin benzoate+lufenuron ME,1 billion PIB/mL of Autographa californica NPV SC and 10%alpha-cypermethrin+lufenuron EC had good persistence,and the control effect at 10 d post spraying was greater than 90%.[Conclusion]Compound preparations of indoxacarb and tebufenozide or methoxyfenozide are recommended for controlling lepidopteran pests of cauliflower.

关键词： cauliflower Lepidopteran pests Insecticide Indoxacarb Control effect

Plant infection by two different viruses induce contrasting changes of vectors fitness and behavior

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2019年第1期26卷 86-96页

作者： Quentin Chesnais Aude Couty Maryline Uzest Veronique Brault Arnaud Ameline FRE CNRS 3498 EDYSAN (Ecologie et Dynamique des Systemes Anthropises) Universite de Picardie Jules Veme33 rue St Leu80039 Amiens CedexFrance INRA UMR 0385 BGPICIRAD-INRA-Montpellier SupAgroTA A541KCampus International de Baillarguet34394 Montpellier Cedex 5France UMR 1131 SVQV INRA-UDS28rue de Herrlisheim68021 Colmar CedexFrance

Insect-vectored plant viruses can induce changes in plant phenotypes,thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e.,circulative vs.noncirculative).This indirect vector manipulation requires host-virus-vector coevolution and would thus be effective solely in very specific plant-virus-vector species associations.Some studies suggest this manipulation may depend on multiple factors relative to various intrinsic characteristics of vectors such as transmission efficiency.In anintegrative study,we tested the effects of infection of the Brassicaceae Camelina sativa with the noncirculative cauliflower mosaic virus (CaMV)or the circulative Turnip yellows virus (TuYV)on the host-plant colonization of two aphid species differing in their virus transmission efficiency:the polyphagous Myzus persicae,efficient vector of both viruses,and the Brassicaceae specialist Brevicoryne brassicae,poor vector of TuYV and efficient vector of CaMV.Results confirmed the important role of virus mode of transmission as plant-mediated effects of CaMV on the two aphid species induced negative alterations of feeding behavior (i.e.,decreased phloem sap ingestion)and performance that were both conducive for virus fitness by promoting dispersion after a rapid acquisition.In addition,virus transmission efficiency may also play a role in vector manipulation by viruses as only the responses of the efficient vector to plant-mediated effects of TuYV,that is,enhanced feeding behavior and performances,were favorable to their acquisition and further dispersal.Altogether,this work demonstrated that vector transmission efficiency also has to be considered when studying the mechanisms underlying vector manipulation by viruses.Our results also re- inforce the idea that vector manipulation requires coevolution between plant,virus and vector.

关键词： aphid vector cauliflower mosaic virus electrical penetration graph host-plant selection life history traits Turnip yellows virus

Improving the Nutritional Values of Wheat and Rice Crackers by Using cauliflowers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2021年第6期12卷 643-658页

作者： Dalia M. El Sheikh Manal S. Helal Heba A. Barakat Food Technology Research Institute Agricultural Research Center Giza Egypt

The present study described the enhancement of the nutritional values of wheat and rice crackers by adding cauliflowers. The results showed that 25% - 75% cauliflower replaced in crackers increased the consumer acceptability, nutritional value, antioxidant activity, minerals, and anti-carcinogenic effect. Moreover, it also improved the functional properties and sensory quality. The results revealed that the raw samples of cauliflower recorded the highest value of moisture, ash, protein and fiber 89.29%, 7.68%, 19.04% and 10.18% on dry weight, respectively. The crackers formula of cauliflower: wheat 75%:25% and cauliflower: rice 75%:25% recorded the highest values in ash, protein, fat, and fiber. The data showed that increasing cauliflower percentage in crackers formula causing a parallel increase in all determined chemical composition. cauliflower samples exhibited the highest antioxidant activity (92.94%). Also, crackers samples of cauliflower: wheat 75%:25% crackers, recorded 57.6% for antioxidant activity. The highest values of phenols and flavonoids for cauliflower cracker samples (cauliflower: wheat 75%:25% and cauliflower: rice 50%:50%) have increased up to (5.75 and 5.49) respectively. The results showed that the higher mineral content is the sulfur (635.96 mg/100g) in raw cauliflower, while (1.00 mg/100g, and 0.99 mg/100g sulfur content) in wheat and rice respectively. Moreover, the result found that as the cauliflower percentage increases, the minerals contents especially sulfur increase. Furthermore, the cauliflower displayed moderate activity for human hepatocellular carcinoma, colon cancer and intestinal epithelial barrier treatment, (IC50 = 183.4 ± 6.8, 175.8 ± 7.1 and 207.2 ± 8.5 μg/mL, respectively). There were significant differences in the taste property;the highest degree of acceptance of the taste was obtained to crackers samples which were replaced with cauli

关键词： cauliflower Wheat Rice Crackers Phenols Flavonoids Antioxidants Hepatocellular Colon intestinal Glutenfree

Chemically Induced Mutants of Brassica oleracea var. botrytisMaintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第3期4卷 498-507页

作者： Fazal Hadi Michael P. Fuller Plant Physiology Laboratory School of Biological and Biomedical Sciences University of Plymouth Plymouth UK

Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of cauliflower were created and screened for drought and salt stress tolerance. The highly tolerant mutants were selected, regenerated by tissue culture techniques, screened again for drought and salt tolerance under in-vitro and in-vivo conditions, correlated the response of in-vitro and in-vivo plants within a clone. Free proline levels in clones were correlated with stress tolerance. Results confirmed the persistence of mutations in clones with enhanced resistance levels to stresses over control plants. The regenerated in-vitro and in-vivo plants within a clone showed a positive significant correlation for drought (R2 = 0.663) and salt (R2 = 0.647) resistance that confirms the stability of mutation in clones after generations. Proline showed a positive and significant correlation with drought (R2 = 0.524) and salt (R2 = 0.786) tolerance. Conclusively, drought and salt resistance can be successfully enhanced in cauliflower by chemical mutagenesis. Further molecular analysis is recommended to study these mutants.

关键词： Drought Salt Stress Resistance Proline In-Vitro In-Vivo cauliflower