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Impact of ovariectomy and estradiol-17β(E_(2))replacement on the brain steroid levels of the Indian stinging catfish Heteropneustes fossilis

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Aquaculture and Fisheries 2022年第5期7卷 474-483页

作者： Surabhi Mishra Radha Chaube Zoology and Fisheries Department Dolphin(PG)College of Science and AgricultureChunni KalanFatehgarh SahibPunjab140406India Department of Zoology Institute of ScienceBanaras Hindu UniversityVaranasi221005India

In the present study,effects of ovariectomy(Ovx)and estradiol-17β(E2)replacement on the brain steroid levels were investigated in the resting and prespawning phases of the female catfish Heteropneustes fossilis.The study showed that Ovx resulted in significant decreases in plasma levels of estradiol-17β(E2)and its precursors,and the effect varied with the reproductive stage and duration.Our study showed that E2 supplementation reversed the effect of Ovx in 3-week Ovx fish group,either restored or increased according to the dose.In contrast,E2 levels increased in the brain after Ovx in the resting stage and produced a biphasic effect of significant decreases on week 1 and 2,and significant increases on week 3,4 and 5 in the pre-spawning stage.The brain E2 levels significantly inhibited in E2 supplementation groups.The testosterone(T)levels showed biphasic effects,an initial decrease(up to 3 weeks Ovx)and an increase later(week 4,5)in the resting stage.In the pre-spawning stage,the T levels increased significantly after Ovx.The E2 supplementation significantly inhibited the T levels,more severely in the low dose group in the resting and pre-spawning stages.Brain 17-hydroxyprogesterone(17-OHP4)levels increased(week 1,2,3)but decreased on week 4 and 5 of Ovx in the resting stage and increased throughout Ovx in the pre-spawning stage except in the 5th week(though compared to other steroids,level was very low;almost negligible).The E2 replacement significantly decreased the steroid levels further down compared to the Ovx control group.Brain progesterone(P4)and pregnenolone(P5)levels decreased initially(week 1 and 2 Ovx)and recovered later.The E2 replacement significantly decreased P4 levels in the Ovx fish in the pre-spawning stage and in the lower dose group in the resting stage.The P5 levels remained inhibited after the E2 supplementation in the resting stage but unchanged or increased it in the pre-spawning stage.Brain cortisol(F)levels were inhibited initially(1 and 2 weeks and later increased after OVX in the rest

关键词： catfish Neurosteroids Ovariectomy Estrogen replacement Seasonal variations

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A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish(Pelteobagrus fulvidraco)

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Science Bulletin 2018年第21期63卷 1420-1430页

作者： Cheng Dan Qiaohong Lin Gaorui Gong Tianyi Yang Shuting Xiong Yang Xiong Peipei Huang Jian-Fang Gui Jie Mei College of Fisheries Huazhong Agricultural University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences University of Chinese Academy of Sciences

The sex-determining genes are found to be variable among different fish species. Yellow catfish(Pelteobagrus fulvidraco) is an important aquaculture fish species in China with XX/XY sex-determining type. Recently, YY super-male yellow catfish has been successfully produced by combining hormonalinduced sex reversal method with sex chromosome-linked markers. Here, we identified a novel PDZ domain-containing gene in yellow catfish designated as pfpdz1, in whose intron the sex-linked marker was located. The coding sequence of pfpdz1 in Y chromosome was identical to that in X chromosome except a missense SNP(A/T) that changes an amino acid(E8V) in the N-terminal region. Pfpdz1 displayed male-specific expression during sex differentiation. Overexpression of pfpdz1 using additive transgenesis induces XX ovary to differentiate into testis-like tissue, while the targeted inactivation of pfpdz1 in Y chromosome using CRISPR/Cas9-mediated mutagenesis triggers ovarian differentiation. Furthermore,we demonstrated that pfpdz1 initiates testicular differentiation through upregulating expression of amh, dmrt1 and sox9a1, as well as downregulating expression of cyp19a1, foxl2 and wnt4. Our data provide functional evidence that pfpdz1 is significant for male differentiation and maintenance in yellow catfish.

关键词： Sex differentiation Yellow catfish Pfpdz1 Sex reversal

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Cloning,expression pattern,and potential role of MAPKp38 and NF-κBp65 in response to lipopolysaccharide in yellow catfish(Pelteobagrus fulvidraco)

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Animal Nutrition 2019年第4期5卷 416-423页

作者： Dan Shi Ye Zhao Lin Feng Yang Liu Wei-Dan Jiang Pei Wu Juan Zhao Jun Jiang Xiao-Qiu Zhou College of Animal Science and Technology Sichuan Agricultural UniversityChengdu611130China Animal Nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety in Production Sichuan University Key Laboratory Sichuan Agricultural UniversityChengdu611130China

Mitogen-activated protein kinases(MAPK) and nuclear factor kappa B(NF-κB) pathways are considered to be two crucial intracellular signaling cascades in pro-inflammatory responses.In this study,we reported the coding sequences(CDS) of MAPKp38 and NF-κBp65 from yellow catfish.We also investigated the gene structure,expression patterns and functional role in yellow catfish.The CDS of MAPKp38 is1,086 bp encoding 361 amino acids(AA).The MAPKp38 protein has a long highly conserved serine/threonine protein kinases catalytic domain.The NF-κBp65 CDS is 1,794 bp,and the gene encodes 597 AA,with a Rel homology domain(RHD) which consists of a RHD-DNA-binding domain and an Ig-like,plexins,transcription factors(IPT) domain.Moreover,MAPKp38 and NF-κBp65 protein of bony fish and other vertebrates have a single clade.Quantitative real-time PCR analysis revealed the presence of the MAPKp38 and NF-κBp65 transcript in 12 tissues of healthy yellow catfish.The highest expression levels of MAPKp38 and NF-κBp65 were detected in the heart and liver,respectively.Upon stimulation with an intraperitoneal injection of lipopolysaccharide(LPS),the expression levels of MAPKp38 and NF-κBp65 were up-regulated in the intestine.These results indicated that MAPKp38 and NF-κBp65 play important roles in mediating the response protection against LPS in yellow catfish.

关键词： Mitogen-activated protein kinases Nuclear factor kappa B Lipopolysaccharide Expression pattern Yellow catfish

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Optimization of process conditions for drying of catfish(Clarias gariepinus)using Response Surface Methodology(RSM)

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Food Science and Human Wellness 2019年第1期8卷 46-52页

作者： Elijah George Ikrang Kingsley Charles Umani Department of Agricultural and Food Engineering Faculty of EngineeringUniversity of UyoUyoAkwa Ibom StateNigeria Department of Agricultural Engineering Faculty of EngineeringAkwa Ibom State UniversityMkpat EninAkwa Ibom StateNigeria

Drying is applied to lower the moisture content of fish to a level that can prevent the growth of mould and infestation of micro-organism and thus minimizes microbial degradation.The goal of this work was to optimize the process conditions during electrical oven drying of catfish.Moisture content(MC)was quantitatively investigated during the drying process of catfish sizes using response surface methodology(RSM)to obtain minimum moisture content.The independent process variables for the drying process were temperature(50–70◦C),product thickness(10–20 mm),salt concentration(0–20%)and drying time(480–600 min.).Two factorial interaction(2FI)regression model describing the effects of independent drying process variables on the moisture content was developed.The effects of temperature and drying time were more pronounced for MC than the thickness and salt concentration.The optimum conditions were found to be temperature=63.43◦C,product thickness=14.81 mm,salt concentration=9.07%and drying time=600 min.At these optimum conditions,moisture content was found to be 2.64%w.b.Validation of experimental results with the empirical model was evaluated using coefficient of correlation(R2)which was found for the model equation as,R2=0.994.

关键词： Optimization Oven drying Drying parameters catfish Response surface methodology

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RHOPORHYNCHUS LINI N.G.N.SP.(BUCEPHALIDAE: BUCEPHALINAE) FROM THE SWIM BLADDER OF YELLOW catfish, PSEUDOBAGRUS FULVIDRACO

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Chinese Journal of Oceanology and Limnology 1998年第4期16卷 373-376页

作者：王桂堂王伟俊

This paper describes and discuases a new bucephalid species, Rhoporhynchus lini,from the swim bladder of yellow catfish, Pseudobagrous fulvidraco, for Nanhai County of GuangdngProvince in China. lt is chimterized driuly by its wide flat foliate body, largr size, degeneratedrhynchus, voldrinous saccular intestine, dendrihc vitellaria, short uterus, limited locality of the repro-ductive organs and site of infechon in fish swim bladder. It is certainly allocated to the subfamily Bu-cephalinae according to its relative locallty of oveq and testes (ovary pretesticular). But it differs evident-ly from the species in all other genera of the subfamily. Therefore, a new genus, Rhoporhynchus, iserected with R. lini as the type species.

关键词： new genus new species Roporhynchus Rhoporhynchus lini Bucephalidae yellow catfish

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Lipid Oxidation of catfish (<i>Arius maculatus</i>) after Cooking and Smoking by Different Methods Applied in Cameroon

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Food and Nutrition Sciences 2013年第9期4卷 176-187页

作者： Noel Tenyang Hilaire Macaire Womeni Bernard Tiencheu Nand Hrodrik Takugan Foka Félicité Tchouanguep Mbiapo Pierre Villeneuve Michel Linder Biomolecular Engineering Laboratory (LIBio) Université de Lorraine ENSAIA Vandoeuvre-les-Nancy France Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon Lipotechny Laboratory CIRAD Montpellier France.

The influence of different cooking and smoking methods (boiling, frying, frying + boiling, hot and cold smoking) on oxidation and fatty acid composition of catfish (Arius maculatus) commercialized on the Wouri river coast was evaluated including commonly used analytical indexes (peroxide and acid values)and physical analysis by Fourier transform infrared (FTIR) spectroscopy. The mean moisture, protein, ash and fat contents of raw fish were 75.88 ± 0.55;64.24 ± 0.14;10.98 ± 0.31 and 23.02 ± 0.88 respectively. All these treatments provoke the modification of the quality indexes. Lipid deterioration, thermolysis and lipid oxidation occurred throughout the treatment. Peroxide value decreased for all cooked and smoked catfish, while free fatty acids of smoked sample increased. The FTIR applied to evaluate lipid oxidation in extracted lipid clearly provided a better picture of the oxidation progress and led to a similar conclusion with chemical analysis. Boiled, bleaching hot and bleaching cold smoking were found to be the suitable treatment methods for catfish because they gave it a high nutritive value compared to other smoking methods.

关键词： Cooking Method Lipid Oxidation FTIR Spectroscopy Smoking catfish

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DNA Barcoding of Common Commercial Sea catfish (Genus: Plicofollis) from Kuwait

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American Journal of Molecular Biology 2018年第2期8卷 102-108页

作者： Bahia Al-Zafiri Mahmoud Magdy Ramadan Ahmed Mohamed Ali Mohamed Abdel-Salam Rashed Animal Department Collage of Women Ain Shams University Cairo Egypt Genetics Department Faculty of Agriculture Ain Shams University Cairo Egypt

Kuwait fish market is one of the richest markets of native marine fish species. Sea catfishes are not very important in economic point of view, and only few of them (four species) are present and mistakenly, they all named (Chem). Using DNA barcode technique, the common sea catfish present in the East major fish market (Sharq) was analyzed. Based on the most common species ID databases (Barcoding of life database, BOLD and NCBI database), the most proposal identification that is compatible with major survey in 1997, the sea catfish is Plicofollis tenuispinis, the thin-spin sea catfish with similarity 100% and phylogenetic support of 78% bootstrap value. This is the first application of DNA barcode technique to thin-spine sea catfish of Kuwait.

关键词： DNA Barcoding Commercial Fish Species Sea catfish Kuwait

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Virulence of <i>Aeromonas hydrophila</i>Isolated from Fresh Water catfish

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Journal of Biosciences and Medicines 2019年第1期7卷 1-12页

作者： Patience Temitope Fowoyo Frank Achimugu Microbiology Department Baze University Abuja Nigeria Microbiology Department Salem University Lokoja Nigeria

Background: A large proportion of Nigerians consume fish as the source of protein in their meals. This may be attributed to health factors, preference and affordability for low income earners. The incidence of Aeromonas hydrophila in fresh catfish may constitute a significant health risk to the consumer if there is a horizontal transfer to man as it has been reported to be pathogenic. This study examined the possibility of fresh water catfish being a reservoir of pathogenic Aeromonas hydrophila. Method: Aeromonas hydrophila was isolated from the different organs of fresh water catfish (Clarias gariepinus and Ictalurus punctatus) obtained from Kporoko river in Lokoja. Aeromonas hydrophila was identified using both phenotypic and genotypic methods. The pathogenic traits of the Aeromonas species such as biofilm formation, production of haemolysin, enterotoxin and enzymes were determined. Results: Aeromonas hydrophila occurred in all the examined fish organs (fish, liver, kidney, skin and gut) of Clarias gariepinus but occurred only in the skin, intestine, kidney and gut of Ictalurus punctatus examined, but the incidence of Aeromonas hydrophila was prevalent in the gut of all the fishes analyzed. All the Aeromonas isolates analysed in this study produced biofilm, haemolysins and lipase enzymes. They also produced enterotoxins with values ranging between 0.069 - 1.138. Conclusion: The occurrence of Aeromonas in fresh catfish possessing these pathogenic traits is of great public health significance to man as it indicates the likelihood of man being predisposed to toxigenicity when the toxin concentration reaches a lethal value. It is therefore recommended that the internal organs of fresh catfish be thoroughly cleaned and cooked before consumption.

关键词： Aeromonas Biofilms Fresh Water catfish Enterotoxins

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Occurrence of Cryptosporidium species in catfish(Clarias gariepinus)Harvested from two lakes and artificial ponds in Zaria,Northern Nigeria

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Journal of Coastal Life Medicine 2013年第1期1卷 6-11页

作者： Joy Cecilia Atawodi Armayau Hamisu Bichi Department of Veterinary Public Health and Preventive Medicine Ahmadu Bello UniversityZariaNigeria Department of Applied Zoology Bayero UniversityKanoNigeria

Objective:To determine the occurrence of Cryptosporidium species in catfish(Clarias gariepinus)harvested from two lakes and ponds in Zaria,Kaduna State,Nigeria.Methods:catfish samples(n=200)from two lakes and(n=200)from two private fish ponds were collected and their gills and gastrointestinal tract samples were analysed for Cryptosporidium oocysts using modified Ziehl-Neelsen staining technique was used,followed by microscopy.Results:Oocysts of Cryptosporidium species were found in all the sampling sites with an overall positivity rate of 49.75%.A comparison between the lakes and ponds as sampling sites revealed a statistically significant(Pcatfish intended for human consumption and therefore,underlines the need for public enlightenment to guard against any possible zoonotic transmission.

关键词： Cryptosporidium Oocysts catfish Clarias gariepinus Lakes Ponds Nigeria

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Effect of Heavy Metals Pollution on Protein Biosynthesis in catfish

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Journal of Water Resource and Protection 2013年第5期5卷 555-562页

作者： S. A. Habib A. M. Abou Shehatta Samah Biochemistry Division Damietta Faculty of science Mansoura University Mansoura Egypt Food Inspection Laboratory Damietta Port Damietta Egypt

The present study was carried out to evaluate the pollution and its effect on the quality of catfish. Four sites in Eygpt were chosen for the research, Ras El-Bar (Site 1) as control, Shatta (Site 2), Kafr El-Bateekh (Site 3), and Talkha (Site 4). The research was carried out on water, sediments and catfish (serum and muscles). Nitrite, nitrate and ammonia were determined in water and sediment. Also, RNA and DNA were determined in serum samples and the muscles of the catfish. In addition, the concentrations of heavy metals (Pb, Cd, Fe, Zn and Cu) were estimated in water, sediments and the muscles of catfish. Also, hepatosomatic index, liver water content, condition factor, lipid and protein contents were determined in the fish. The concentrations of nitrite, nitrate and ammonia in water and sediment of Site 4 and the levels of heavy metals especially Pb and Cd in water, sediment and muscle of catfish from Sites 3 and 4 were highly elevated compared to those of the control. On the other hand, DNA, RNA and protein contents in the catfish of Sites 3 and 4 decreased. The results illustrated that, Cd and Pb levels in the muscle of catfish were negatively correlated with DNA, RNA and with the protein contents. In conclusion, the accumulation of heavy metals in catfish tissues therefore, can cause health problems in human after catfish intake.

关键词： catfish Protein Biosynthesis Heavy Metals Pollution

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