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Advances in Microbiology 2024年第2期14卷 137-161页

作者： Anshu Mathur Chandrachur Ghosh Partha Roy Ramasare Prasad Rajesh Pratap Singh Department of Biosciences and Bioengineering Indian Institute of Technology Roorkee Roorkee India

Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, 1H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar (Cyamopsis tetragonoloba) and wheat (Triticum aestivum) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.

关键词： Lentinus squarrosulus AF5 Azo Dyes FTIR 1H NMR catabolism Cytotoxicity

Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis

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Genes & Diseases 2023年第4期10卷 1582-1595页

作者： Yiming Pan Yuyou Yang Mengtian Fan Cheng Chen Rong Jiang Li Liang Menglin Xian Biao Kuang Nana Geng Naibo Feng Lin Deng Wei Zheng Fengmei Zhang Xiaoli Li Fengjin Guo Department of Cell Biology and Genetics Core Facility of Development BiologyChongqing Medical UniversityChongqing 400016China Department of Orthopaedics The 1st Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Laboratory of Stem Cells and Tissue Engineering Chongqing Medical UniversityChongqing 400016China Department of Orthopaedics The 2nd Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Chongqing Medical University Laboratory Animal Center Chongqing Medical UniversityChongqing 400016China

Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progression of osteoarthritis(OA)led us to investi-gate the role of PGRN in the regulation of chondrocyte autophagy.PGRN knockout chondro-cytes exhibited a deficient autophagic response with limited induction following rapamycin,serum starvation,and IL-1b-induced autophagy.PGRN-mediated anabolism and suppression of IL-1b-induced catabolism were largely abrogated in the presence of the BafA1 autophagy inhibitor.Mechanistically,during the process of OA,PGRN and the ATG5eATG12 conjugate form a protein complex;PGRN regulates autophagy in chondrocytes and OA through,at least partially,the interactions between PGRN and the ATG5eATG12 conjugate.Furthermore,the ATG5eATG12 conjugate is critical for cell proliferation and apoptosis.Knockdown or knockout of ATG5 reduces the expression of ATG5eATG12 conjugate and inhibits the chondroprotective effect of PGRN on anabolism and catabolism.Overexpression of PGRN partially reversed this effect.In brief,the PGRN-mediated regulation of chondrocyte autophagy plays a key role in the chondroprotective role of PGRN in OA.Such studies provide new insights into the pathogen-esis of OA and PGRN-associated autophagy in chondrocyte homeostasis.

关键词： Anabolism ATG12 ATG5 Autophagy catabolism Osteoarthritis PGRN

Tasselseed5 encodes a cytochrome C oxidase that functions in sex determination by affecting jasmonate catabolism in maize^FA

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Journal of Integrative Plant Biology 2020年第2期62卷 247-255页

作者： Fei Wang Zhenjiang Yuan Zhiwei Zhao Caixia Li Xin Zhang Huafeng Liang Yawen Liu Qian Xu Hongtao Liu National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Shanghai 200032China Key Laboratory of Plant Stress Biology School of Life SciencesHenan UniversityKaifeng 475004China College of Agronomy Shandong Agricultural UniversityTaian 271018China

Maize(Zea mays L.)is a monoecious grass plant in which mature male and female florets form the tassel and ear,respectively.Maize is often used as a model plant to study flower development.Several maize tassel seed mutants,such as the recessive mutants tasselseed1(ts1)and tasselseed2(ts2),exhibit a reversal in sex determination,which leads to the generation of seeds in tassels.The phenotype of the dominant mutant,Tasselseed5(Ts5),is similar to that of ts2.Here,we positionally cloned the underlying gene of Ts5 and characterized its function.We show that the GRMZM2G177668 gene is overexpressed in Ts5.This gene encodes a cytochrome C oxidase,which catalyzes the transformation of jasmonoyl-L-isoleucine(JA-Ile)to 12 OH-JA-Ile during jasmonic acid catabolism.Consistent with this finding,no JA-Ile peak was detected in Ts5 tassels during the sex determination period,unlike in the wild type.Transgenic maize plants overexpressing GRMZM2G177668 exhibited a tassel-seed phenotype similar to that of Ts5.These results indicate that the JA-Ile peak in tassels is critical for sex determination and that the Ts5 mutant phenotype results from the disruption of this peak in tassels during sex determination.

关键词： Tasselseed5 sex determination affecting jasmonate catabolism

MYC2, MYC3, and MYC4 function additively in wounding-induced jasmonic acid biosynthesis and catabolism

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Journal of Integrative Plant Biology 2020年第8期62卷 1159-1175页

作者： Cuiping Zhang Yunting Lei Chengkai Lu Lei Wang Jianqiang Wu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Jasmonic acid(JA)plays a critical role in plant defenses against insects and necrotrophic fungi.Wounding or lepidopteran insect feeding rapidly induces a burst of JA in plants,which usually reaches peak values within 1 to 2 h.The induced JA is converted to JA-Ile and perceived by the COI1-JAZ co-receptor,leading to activation of the transcription factors MYC2 and its homologs,which further induce JA-responsive genes.Although much is known about JA biosynthesis and catabolism enzymes and JA signaling,how JA biosynthesis and catabolism are regulated remain unclear.Here,we show that in Arabidopsis thaliana MYC2 functions additively with MYC3 and MYC4 to regulate wounding-induced JA accumulation by directly binding to the promoters of genes function in JA biosynthesis and catabolism to promote their transcription.MYC2 also controls the transcription of JAV1 and JAM1,which are key factors controlling JA biosynthesis and catabolism,respectively.In addition,we also found that MYC2 could bind to the MYC2 promoter and self-inhibit its own expression.This work illustrates the central role of MYC2/3/4 in controlling wounding-induced JA accumulation by regulating the transcription of genes involved in JA biosynthesis and catabolism.

关键词： expression. MYC2 catabolism

Mediated Electrochemical Measurements of Intracellular Catabolic Activities of Yeast Cells

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Chinese Chemical Letters 2005年第4期16卷 513-516页

作者： JinShengZHAO ZhenYuYANG YaoLU ZhengYuYANG TechnicalInstituteofPhysicsandChemistry ChineseAcademyofSciencesBeijing100101

Coupling with the dual mediator system menadione/ferricyanide, microelectrode voltammetric measurements were undertaken to detect the ferrocyanide accumulations arising from the mediated reduction of ferricyanide by yeast cells. The results indicate that the dual mediator system menadione/ferricyanide could be used as a probe to detect cellular catabolic activities in yeast cells and the electrochemical response has a positive relationship with the specific growth rate of yeast cells.

关键词： catabolism intracellular microelectrode votammetric mediator yeast cells.

The Sucrose Starvation Signal Mediates Induction of Autophagy- and Amino Acid catabolism-Related Genes in Cowpea Seedling

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American Journal of Plant Sciences 2013年第3期4卷 647-653页

作者： Ayami Kaneko Eri Noguchi Yushi Ishibashi Takashi Yuasa Mari Iwaya-Inoue Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fukuoka Japan Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fukuoka Japan. Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan School of Agriculture Kyushu University Fukuoka Japan

In higher plants, autophagy is bulk degradation process in vacuole necessary for survival under nutrient-limited conditions and plays important roles in senescence, development and pathogenic response, etc. Cowpea is one of the most important legume crops in semi-aride region, which is highly tolerant to drought stress. Changes of photoassimilate status by drought stress and/or sink-source balance appeared to affect autophagy and senescence of leaf in cowpea. Accordingly, we focused on roles of sucrose signal in autophagy and amino acid recycling in cowpea. Effects of starvation stress on the expression of autophagy-related genes (ATGs) and amino acid catabolism-related genes in cowpea [Vigna unguiculata (L.) Walp] were examined by Reverse transcription-polymerase chain reaction (RT-PCR) and anti-ATG8i specific antibody. Sucrose starvation stress enhanced the expression levels of VuATG8i, VuATG8c and VuATG4 incowpea seedlings. The expressions of amino acid catabolism related genes, such as asparagine synthase (VuASN1), proline dehydrogenase1 (VuProDH) and branched chain amino acid transaminase (VuBCAT2), are also up-regulated under the sucrose starvation. In contrast, high sucrose condition suppressed autophagy and the expressions of ATGs. These results indicate that sucrose starvation stress stimulates both autophagy and amino acid catabolism by regulation of ATGs and VuBCAT2. It is conceivable that sucrose starvation stress enhances autophagy in cowpea, possibly via branched chain amino acid level regulated by the starvation-induced BCAT.

关键词： Amino acid catabolism ATG Autophagy BCAT Cowpea Starvation Stress

Effects of Glucose Load on catabolism during Propofol-Based Anesthesia with Remifentanil in Patients with Diabetes Mellitus: A Prospective Randomized Trial

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Open Journal of Anesthesiology 2015年第9期5卷 197-205页

作者： Tomotsugu Yamada Maiko Hasegawa-Moriyama Mayumi Nakahara Akira Matsunaga Yuichi Kanmura Department of Anesthesiology and Critical Care Medicine Graduate School of Medical and Dental Sciences Kagoshima University Kagoshima Japan

Background: Perioperative exacerbation of hyperglycemia and insulin resistance is associated with increased complications in patients with diabetes mellitus. We recently reported that glucose load during anesthesia with sevoflurane suppressed lipid catabolism in diabetic patients. In contrast to inhaled anesthetics, propofol solution contains triglycerides, which can be an energy source during surgery. However, the clinical relevance of glucose load under propofol-based anesthesia in diabetic patients is unknown. Therefore, we investigated the effect of intraoperative glucose load on catabolism during propofol-based anesthesia in patients with diabetes mellitus. Methods: Twenty-three patients with diabetes mellitus undergoing elective surgery with propofol-remifentanil-based anesthesia were randomly assigned to receive a glucose load (1.5 mg/kg/ min) or not. Plasma levels of glucose, insulin, cortisol, catecholamines, acetoacetic acid, free fatty acids, ketone bodies, 3-hydroxybutyric acid, and 3-methylhistidine/creatinine, used as a marker for protein catabolism, were measured at the start of surgery and 3 h later. Results: Glucose and insulin levels were significantly higher in patients who received a glucose load than in those who did not. Nonetheless, the levels of cortisol and catecholamines were unchanged during surgery. Similarly, the difference in the levels of markers for lipid as well as protein catabolism was not significant between the groups at 3 h after the start of surgery. Conclusions: Changes in lipid as well as protein catabolism were not altered by glucose load in diabetic patients under propofol-based anesthesia with remifentanil. Our study suggested that continuous infusion of propofol at a clinical dose is sufficient to reduce the requirement for glucose infusion during surgery in patients with diabetes.

关键词： Diabetes Propofol Glucose Infusion catabolism

The Effects of Glucose Load on catabolism during Remifentanil-Based Anesthesia in Patients with Diabetes Mellitus: A Prospective Randomized Trial

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Open Journal of Anesthesiology 2015年第7期5卷 155-162页

作者： Maiko Hasegawa-Moriyama Mayumi Nakahara Takayuki Saito Tomotsugu Yamada Keika Mukaihara Mina Masuda Akira Matsunaga Yuichi Kanmura Department of Anesthesiology and Critical Care Medicine Graduate School of Medical and Dental Sciences Kagoshima University Kagoshima Japan

Background: General anesthesia using remifentanil may suppress the unwanted metabolic changes caused by surgical stress including hyperglycemia and ketogenesis. Surgery-related changes in catabolism can be attenuated with low-dose glucose load, without causing hyperglycemia. However, the impact of glucose load in diabetic patients during surgery is unknown. In this study, we investigated the effect of glucose load on catabolism during remifentanil-based anesthesia in patients with diabetes mellitus. Methods: Twenty-nine patients with diabetes mellitus undergoing elective surgery were randomly assigned to receive a glucose load (1.5 mg/kg/min) or not. Plasma levels of glucose, insulin, cortisol, dopamine, adrenaline, noradrenaline, acetoacetic acid, free fatty acid, ketone bodies, 3-hydroxybutyric acid, and 3-methylhistidine/creatinine, a marker of protein catabolism were measured at the start of surgery and 3 h after the start of surgery. Results: Glucose and insulin levels were significantly higher in patients who received a glucose load than in those who did not. Cortisol levels decreased at 3 h after the start of surgery in both groups whereas the levels of catecholamines were unchanged. Acetoacetic acid and total ketone body levels were significantly lower in patients given a glucose load than in those who were not 3 h after the start of surgery. The difference in the 3 methylhistidine/creatinine ratio between the two groups was not significant. Conclusions: The infusion of glucose suppressed lipid catabolism in diabetic patients under remifentanil-based anesthesia during surgery. Our study also suggests that in patients with diabetes mellitus, protein sparing is inhibited by remifentanil-based anesthesia, regardless of the glucose load. Trial registration: the University Hospital Medical Information Network identifier: UMIN000010914.

关键词： Diabetes Remifentanil Glucose Infusion catabolism

Late immune consequences of combat trauma: A review of trauma-related immune dysfunction and potential therapies

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Military Medical Research 2019年第4期6卷 340-353页

作者： Kelly B.Thompson Luke T.Krispinsky Ryan J.Stark Division of Critical Care Medicine Department of PediatricsVanderbilt University School of Medicine2200 Children’s WayNashvilleTN 37232USA Division of Pediatric Critical Care Medicine Department of PediatricsUniformed Services UniversityNaval Medical Center PortsmouthPortsmouthVA 23708USA

With improvements in personnel and vehicular body armor,robust casualty evacuation capabilities,and damage control resuscitation strategies,more combat casualties are surviving to reach higher levels of care throughout the casualty evacuation system.As such,medical centers are becoming more accustomed to managing the deleterious late consequences of combat trauma related to the dysregulation of the immune system.In this review,we aim to highlight these late consequences and identify areas for future research and therapeutic strategies.Trauma leads to the dysregulation of both the innate and adaptive immune responses,which places the injured at risk for several late consequences,including delayed wound healing,late onset sepsis and infection,multi-organ dysfunction syndrome,and acute respiratory distress syndrome,which are significant for their association with the increased morbidity and mortality of wounded personnel.The mechanisms by which these consequences develop are complex but include an imbalance of the immune system leading to robust inflammatory responses,triggered by the presence of damage associated molecules and other immune-modifying agents following trauma.Treatment strategies to improve outcomes have been difficult to develop as the immunophenotype of injured personnel following trauma is variable,fluid and difficult to determine.As more information regarding the triggers that lead to immune dysfunction following trauma is elucidated,it may be possible to identify the immunophenotype of injured personnel and provide targeted treatments to reduce the late consequences of trauma,which are known to lead to significant morbidity and mortality.

关键词： Trauma Sepsis Compensatory anti-inflammatory response syndrome Persistent inflammationimmunosuppression and catabolism syndrome Immune dysfunction

Metabolic features of cancer cells

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Cancer Communications 2018年第1期38卷 695-700页

作者： Yugang Wang Yan Xia Zhimin Lu Brain Tumor Center and Department of Neuro-Oncology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA Department of Molecular and Cellular Oncology The University of Texas MD Anderson Can-cer CenterHoustonTX 77030USA Cancer Biology Program The University of Texas MD Anderson Cancer Center UT Health Graduate School of Biomedical SciencesHoustonTX 77030USA

Cancer cells uniquely reprogram their cellular activities to support their rapid proliferation and migration and to coun-teract metabolic and genotoxic stress during cancer progression.In this reprograming,cancer cells’metabolism and other cellular activities are integrated and mutually regulated,and cancer cells modulate metabolic enzymes spatially and temporally so that these enzymes not only have altered metabolic activities but also have modulated subcellular localization and gain non-canonical functions.This review and several others in this issue of Cancer Communications discuss these enzymes’newly acquired functions and the non-canonical functions of some metabolites as features of cancer cell metabolism,which play critical roles in various cellular activities,including gene expression,anabolism,catabolism,redox homeostasis,and DNA repair.

关键词： Metabolic enzymes Metabolites Non-canonical functions Protein kinase Lipid metabolism Gene expression Anabolism catabolism Redox homeostasis DNA repair