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Chinese Chemical Letters 2019年第2期30卷 443-446页

作者： Yujiao Zhang Kebo Xie Aijing Liu Ridao Chen Dawei Chen Lin Yang Jungui Dai College of Life and Environmental Sciences Minzu University of China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Enzymatic glycosylation catalyzed by glycosyltransferases (GTs) has great potential in creating diverse novel and bioactive glycosides. Herein, three new GTs (UGT84 A33, UGT71 AE1 and UGT90 A14) from carthamus tinctorius exhibited robust catalytic promiscuity to benzylisoquinoline alkaloids, and were used as enzymatic tools in glycosylation of bioactive benzylisoquinoline alkaloids. Seven novel benzylisoquinoline alkaloids O-glycosides were synthesized with high efficiency. These studies indicate the significant potential of promiscuous GTs in synthesis of benzylisoquinoline alkaloids glycosides for drug discovery.

关键词： Glycosyltransferase Catalytic promiscuity Benzylisoquinoline alkaloids carthamus tinctorius

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Protective effect of Salvia miltiorrhiza and carthamus tinctorius extract against lipopolysaccharide-induced liver injury

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World Journal of Gastroenterology 2015年第30期21卷 9079-9092页

作者： Li-Na Gao Kuo Yan Yuan-Lu Cui Guan-Wei Fan Yue-Fei Wang Research Center of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine Research and Development Center of TCM Tianjin International Joint Academy of Biotechnology and Medicine

AIM:To investigate the hepatoprotective effects and mechanisms of an extract of Salvia miltiorrhiza and carthamus tinctorius in ***:C57BL/6J mice were randomly assigned to five groups and intraperitoneally administered 0.9% saline,Salvia miltiorrhiza and carthamus tinctorius extract [Danhong injection(DHI),0.75 and 3 g/kg mixed extract] or reduced glutathione for injection(RGI,300 mg/kg) for 30 min before exposure to lipopolysaccharide(LPS,16 mg/kg). After intraperitoneal LPS stimulation for 90 min or 6 h,the mice were sacrificed by ether anaesthesia,and serum and liver samples were collected. Histological analysis(H&E) and terminal deoxynucleotidyl transferase-mediated d UTP nick end-labelling(TUNEL) staining were performed. Alanine transferase(ALT),aspartate transaminase(AST),total bilirubin(TBil),glutathione-S-transferase(GST),malondialdehyde(MDA),tumour necrosis factor(TNF)-α,interleukin(IL)-6,and caspase-3 levels were measured. Bax,Bcl-2,P-IκBα,IκBα,P-NF-κB p65,and NF-κB p65 protein levels were determined by Western blot. TNF-α,IL-6,caspase-3,Bax and Bcl-2 m RNA expression was measured by real-time reverse transcription-polymerase chain reaction(RT-PCR).RESULTS:Hematoxylin-eosin staining and TUNEL results suggested that DHI(3 g/kg) treatment alleviated inflammatory and apoptotic(P < 0.01) injury in the liver of mice. DHI treatment dose-dependently blunted the abnormal changes in biochemical parameters such as ALT(72.53 ± 2.83 for 3 g/kg,P < 0.01),AST(76.97 ± 5.00 for 3 g/kg,P < 0.01),TBil(1.17 ± 0.10 for 3 g/kg,P < 0.01),MDA(0.81 ± 0.36 for 3 g/kg,P < 0.01),and GST(358.86 ± 12.09 for 3 g/kg,P < 0.01). Moreover,DHI(3 g/kg) remarkably decreased LPS-induced protein expression of TNF-α(340.55 ± 10.18 for 3 g/kg,P < 0.01),IL-6(261.34 ± 10.18 for 3 g/kg,P < 0.01),and enzyme activity of caspase-3(0.93 ± 0.029 for 3 g/kg,P < 0.01). The LPS-induced m RNA expression of TNF-α,IL-6 and caspase-3 was also decreased by DHI. Western blot analysis revealed that DHI an

关键词： Salvia miltiorrhiza carthamus tinctorius Apoptosis Anti-inflammatory Antioxidant Acute liverinjury

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>carthamus tinctorius</i>L.) cv. NARI-H-15

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American Journal of Plant Sciences 2017年第4期8卷 688-705页

作者： Jeyachandran Vijayakumar Ponnirul Ponmanickam P. Samuel D. N. P. Sudarmani J. Pandiarajan Department of Biotechnology Ayya Nadar Janaki Ammal College Sivakasi India Department of Zoology Ayya Nadar Janaki Ammal College Sivakasi India

The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (carthamus tinctorius L.) cv. NARI-H-15. For micropropagation, 7 - 9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system.

关键词： carthamus Tinctorius L. Meta-Topolin N-(2-chloro-4-pyridyl-N’-phenylurea) Micropropagation Organogenesis

Assay for the developmental toxicity of safflower (carthamus tinctorius L.) to zebrafish embryos/larvae

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Journal of Traditional Chinese Medical Sciences 2017年第1期4卷 71-81页

作者： Qing Xia Zhiqiang Ma Xue Mei Jun Luo Yutong Wang Tongtong Li Yaru Feng Yuanyuan Ni Qinwen Zou Ruichao Lin Beijing Key Lab for Quality Evaluation of Chinese Materia Medica School of Chinese PharmacyBeijing University of Chinese MedicineBeijing100102China Department of Quality Control Beijing Lianxin Pharmaceutic Co.Ltd.Beijing102609China

Objective:To explore the developmental toxicity and the potential toxicological mechanism of safflower (carthamus tinctorius L.) on zebrafish embryos/***:Mortality,malformations and increased apoptosis induced by safflower were assessed in zebraflsh embryos from 6 to 96 hours *** and genes in the anti-oxidative and apoptotic pathways were also ***:The lethal concentration 50 of safflower to zebrafish embryos was 345.6 mg/*** inhibition,abnormal spontaneous movement,depressed heart rate,pericardial edema,yolk sac edema,abnormal head-trunk angle,inhibition of melanin release,enlarged yolk,and short body length were observed in safflower-treated *** apoptotic cells mainly appeared around the *** exposure changed the activities of defense enzymes (SOD T,CAT↑,MDA↑,GPX without trend),increased MDA content,decreased caspase-3 activity,and altered mRNA levels of related genes (ogg1 ↓,p53↓,Cu/Zn-sod↑,Mn-sod↓,cat↓,gpx↑).Conclusion:Safflower exhibits developmental toxicity for zebrafish embryos/*** developing heart was speculated as the target organ of *** stress and increased apoptosis have roles in the developmental toxicity of *** article provides a novel method to research the teratogenicity and possible mechanisms of toxicity of traditional Chinese medicines that are prohibited or contraindicated in pregnant women.

关键词： carthamus tinctorius L. Developmental toxicity Zebrafish Apoptosis Oxidative stress

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红花(carthamus tinctrius L.)的生长发育研究 Ⅰ.花器发育的扫描电镜观察(简报)

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中国农业大学学报 1990年第1期 70-70页

作者：徐楚年贾君镇林庆文梁辉

红花是一种古老作物,广泛分布于世界各地。我国主要产区为新疆、河南、四川、浙江等省。它是油、药、饲料、染料兼用的经济作物。红花子油含丰富亚油酸,为优质保健食用油。花可作药用,也可提取天然食用色素。红花具抗旱、耐寒、耐瘠和... 详细信息

红花是一种古老作物,广泛分布于世界各地。我国主要产区为新疆、河南、四川、浙江等省。它是油、药、饲料、染料兼用的经济作物。红花子油含丰富亚油酸,为优质保健食用油。花可作药用,也可提取天然食用色素。红花具抗旱、耐寒、耐瘠和耐盐等特性。我国种植红花以油为主、花用为辅、油花兼用,是开发旱薄地的优势作物。本研究供试材料为美国有刺品种,1988年种植于农大校园内,播期4月16日,按常规田间管理。红花属菊科红花属,在主茎和分枝顶端着生头状花序,又称花球。花球由许多管状花聚

关键词：小花发育雌蕊雄蕊大蕊花部形态小蕊红花属 tinctrius carthamus 扫描电镜观察花器

红花(carthamus tinctorius)染色体组型分析

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兰州大学学报 1985年第2期 82-85页

作者：王亚馥潘志强广东华南热带作物学院进修教师

本实验是应用植物有丝分裂染色体标本制备的新方法,观察了红花的染色体形态、数目以及结构特征,从而进行了染色体组型的初步分析,并对红花染色体的有关特点进行了讨论.红花染色体为2n=24,可配为12对,其中5对为中部着丝点染色体.6对为亚... 详细信息

本实验是应用植物有丝分裂染色体标本制备的新方法,观察了红花的染色体形态、数目以及结构特征,从而进行了染色体组型的初步分析,并对红花染色体的有关特点进行了讨论.红花染色体为2n=24,可配为12对,其中5对为中部着丝点染色体.6对为亚中部着丝点和一对在短臂具随体染色体.

关键词：染色体组型红花油具随体染色体染色体形态 carthamus 着丝点细胞学研究标本制备硬粒应用植物

HPLC法测定红花地上部分羟基红花黄色素A和木犀草素的含量

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中国民族民间医药 2014年第8期23卷 29-30页

作者：王秀梅韩晓静白梅荣关永仙内蒙古民族大学内蒙古通辽028000

目的:建立红花地上部分中羟基红花黄色素A和木犀草素的含量测定方法。方法:采用HPLC法,色谱柱为Eclipse plusC18柱(5μm,4.6mm×150mm),流动相为甲醇-0.7%磷酸梯度洗脱,流速:1.0 ml/min,检测波长390nm,柱温30℃。结果:羟基红花黄色素A在... 详细信息

目的:建立红花地上部分中羟基红花黄色素A和木犀草素的含量测定方法。方法:采用HPLC法,色谱柱为Eclipse plusC18柱(5μm,4.6mm×150mm),流动相为甲醇-0.7%磷酸梯度洗脱,流速:1.0 ml/min,检测波长390nm,柱温30℃。结果:羟基红花黄色素A在0.03μg/ml^0.19μg/ml,(r=0.999 9);木犀草素在0.13μg/ml^0.80μg/ml,(r=0.999 5)范围内有良好的线性关系,平均回收率为99.99%,98.95%(n=6)。结论:该方法简便、快捷、准确、重复性好,可用于羟基红花黄色素A和木犀草素的含量测定。

关键词：红花高效液相色谱法木犀草素含量测定 carthamus tinctorius L

Phytometric and Productive Characteristics of Safflower Submitted to Phosphate Fertilization in the Oxisol of the Brazilian Cerrado

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American Journal of Plant Sciences 2017年第12期8卷 2966-2976页

作者： Edna Maria Bonfim-Silva Luiz Fernando Santos Miranda Laura Cristina Rezende das Neves Helon Hébano de Freitas Sousa Jefferson Vieira José Department of Agricultural Engineering Federal University of Mato Grosso Rondonópolis Brazil

The safflower (carthamus tinctorius L.), in particular, has been soaring in significance as an alternative for oil and biodiesel production. In this context, the goal of this study was to assess the influence exerted by phosphate fertilization on the phytometric and productive characteristics of the safflower raised on the Oxisol of the Cerrado. The experiment was performed in a greenhouse, using pots of 3 dm-3?in volume. Adopting the completely randomized design, five doses of phosphorus (P2O5: 0, 90, 180, 360 and 540 mg·dm-3) and six replicates were included. The following variables were estimated, viz., number of leaves, plant height, and stem diameter, besides chlorophyll index (SPAD index), number of chapters and shoot dry matter. Barring the shoot dry matter and number of chapters, all the other variables were evaluated on days 30 and 45 after the plants were transplanted. Data were submitted to analysis of variance at 1%, 5% and 10% probabilities, and regression analysis, at 1% and 5% probabilities, respectively. All the variables revealed significant differences and adjustments to the linear regression model (P < 0.1), excepting the SPAD index on day 30 after the plant emergence which was adjusted to the quadratic regression model. The highest findings of 24.47 for leaf number, 34.97 cm for plant height, 3.76 mm for stem diameter, 64.76 for chlorophyll index, 5 units for the number of chapters and 3.45 g for dry shoot mass were recorded in response to the highest phosphorus dose in the experimental range (P2O5: 540 mg·dm-3). Phosphate fertilization was observed to affect the phytometric characteristics and boost the development of the safflower plants cultivated in the Cerrado Oxisol, substantiating that safflower could be a viable cash crop that can be established in the Brazilian Cerrado.

关键词： carthamus tinctorius L. Alternative Culture for Biodiesel Oleaginous

Soil Water Availabilities in the Content and Accumulation of Nitrogen and Chlorophyll Index in the Safflower

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American Journal of Plant Sciences 2015年第14期6卷 2280-2286页

作者： Edna Maria Bonfim-Silva Ellen Cristina Alves de Anicésio Jakeline Rosa de Oliveira Helon Hébano de Freitas Sousa Tonny José Araújo da Silva Department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and Technology Federal University of Mato Grosso Rondonópolis Brazil

The safflower (carthamus tinctorius L.) is adapted to grow in adverse conditions, is tolerant to salinity and water deficit, however, its productivity increases when grown in areas with greater availability of water and in fertilized conditions, showing that the knowledge of crop management can bring income gains for producers. It is known that extreme conditions of soil moisture damage the crop development. Given this context, the objective was to evaluate the influence of water availability on the nutritional characteristics of the safflower. The experiment was conducted in a greenhouse in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity of the soil) in four replications. Maintaining soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were SPAD reading (chlorophyll content), concentration and accumulation of nitrogen in the shoot and in the sections. The results were submitted to analysis of variance and regression test at 5% probability by Sisvar program. There was adjusting of variables to linear and quadratic regression models. Water availabilities between 65% and 75% have greater potential to promote better nutrition for safflower plants. Both deficit and excess of water in the soil are detrimental to the absorption of nitrogen and reduce the chlorophyll content. Safflower is less tolerant to excess than to deficit of water.

关键词： carthamus tinctorius L. Nutrition SPAD Irrigation

Soil Water Availability on Growth and Development of Safflower Plants

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American Journal of Plant Sciences 2015年第13期6卷 2066-2073页

Safflower (carthamus tinctorius L.) is a promising culture to be widespread in Brazil. However, the lack of basic knowledge about cultivation techniques, such as water demand by the culture, is still obstacle to the expansion of safflower in that country. The objective was, then, to evaluate the effect of the soil water availability on growth and development of safflower in the Cerrado soil of Mato Grosso, Brazil. The experiment was conducted in a greenhouse, in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity in the soil) and four replications. Maintenance soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were: plant height, stem diameter, number of leaves, number of heads, heads diameter, dry mass of shoots, heads, and roots. The results were submitted to analysis of variance and regression test at 5% probability by SISVAR program. All variables set to the quadratic regression model, showing the best results in the water availability between 64% and 76%. Safflower is shown to be more sensitive to water stress with increased tolerance to water deficit in the soil than to flooding.

关键词： carthamus tinctorius L. Field Capacity Water Stress IMA 0213