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High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第4期22卷 285-294页

作者： Lei XU Zhifang WU Yuan WANG Sa WANG Chang SHU Zhuhui DUAN Shuli DENG The Affiliated Hospital of Stomatology School of StomatologyZhejiang University School of Medicineand Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhou 310006China Department of Stomatology Luoyang Central Hospital Affiliated to Zhengzhou UniversityLuoyang 471009China

Objectives:The study aimed at identifying salivary microbiota in caries-free Chinese preschool children using highthroughput ***:Saliva samples were obtained from 35 caries-free preschool children(18 boys and 17 girls)with primary dentition,and 16 S ribosomal DNA(r DNA)V3–V4 hypervariable regions of the microorganisms were analyzed using Illumina ***:At 97%similarity level,all of these reads were clustered into 334 operational taxonomic units(OTUs).Among these,five phyla(Firmicutes,Proteobacteria,Actinobacteria,Bacteroidetes,and Candidate division TM7)and13 genera(Streptococcus,Rothia,Granulicatella,Prevotella,Enterobacter,Veillonella,Neisseria,Staphylococcus,Janthinobacterium,Pseudomonas,Brevundimonas,Devosia,and Gemella)were the most dominant,constituting 99.4%and 89.9%of the salivary microbiota,*** core salivary microbiome comprised nine genera(Actinomyces,Capnocytophaga,Gemella,Granulicatella,Lachnoanaerobaculum,Neisseria,Porphyromonas,Rothia,and Streptococcus).Analysis of microbial diversity and community structure revealed a similar pattern between male and female *** difference in microbial community composition between them was mainly attributed to Neisseria(P=0.023).Furthermore,functional prediction revealed that the most abundant genes were related to amino acid transport and ***:Our results revealed the diversity and composition of salivary microbiota in caries-free preschool children,with little difference between male and female *** of the core microbiome,coupled with prediction of gene function,deepens our understanding of oral microbiota in cariesfree populations and provides basic information for associating salivary microecology and oral health.

关键词： Salivary microbiota caries-free Preschool children Primary dentition Illumina MiSeq 16S rDNA V3-V4 hypervariable regions

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Asian Pacific Journal of Tropical Biomedicine 2016年第8期6卷 720-726页

作者： Amina Hoceini Nihel Klouche Khelil Ilhem Ben-Yelles Amine Mesli Sara Ziouani Lotfi Ghellai Nadia Aissaoui Fatima Nas Mounia Arab Laboratory of Applied Microbiology in Food Biomedical and Environment Department of Biology Faculty of Nature and Life University of Tlemcen Laboratory of Experimental Surgery Dental Surgery Department Faculty of Medicine University of Tlemcen Laboratory of Applied Molecular Biology and Immunology Department of Biology University of Tlemcen

Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacterial composition of the supragingival plaques from the above-mentioned ***: Participants completed a questionnaire and were clinically examined for dental caries using decayed, missing and filled teeth index according to the criteria laid down by the World Health Organization. Supragingival plaque samples were collected from 50 caries-free adults(CF) and 50 caries-active adults(CA). Standard procedures of culture and identification of aerobic and anaerobic bacteria were used. Data were analyzed using Chi-square ***: A total of 117 bacterial strains were isolated from supragingival plaques in CF group subjects, 76(64.96%) of them belonged to 9 aerobic genera, and 41(35.04%) to 9anaerobic genera(P caries formation, and a direct association of sugar intake and cultural level with dental ***, oral hygiene practices minimize the prevalence of tooth decay.

关键词： Supragingival plaque caries-free caries-active Bacterial composition Algerian adults Cultural level Food intake

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