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carbonylation and Loss-of-Function Analyses of SBPase Reveal Its Metabolic Interface Role in Oxidative Stress, carbon assimilation, and Multiple Aspects of Growth and Development in Arabidopsis

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Molecular Plant 2012年第5期5卷 1082-1099页

作者： Xun-Liang Liu Hai-Dong Yu Yuan Guan Ji-Kai Li Fang-Qing Guo The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai) Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China

Sedoheptulose-l,7-bisphosphatase （SBPase） is a Calvin cycle enzyme and functions in photosynthetic carbon fixation. We found that SBPase was rapidly carbonylated in response to methyl viologen （MV） treatments in detached leaves of Arabidopsis plants. In vitro activity analysis of the purified recombinant SBPase showed that SBPase was car- bonylated by hydroxyl radicals, which led to enzyme inactivation in an H202 dose-dependent manner. To determine the conformity with carbonylation-caused loss in enzymatic activity in response to stresses, we isolated a loss-of-function mutant sbp, which is deficient in SBPase-dependent carbon assimilation and starch biosynthesis, sbp mutant exhibited a severe growth retardation phenotype, especially for the developmental defects in leaves and flowers where SBPASE is highly expressed. The mutation of SBPASE caused growth retardation mainly through inhibition of cell division and ex- pansion, which can be partially rescued by exogenous application of sucrose. Our findings demonstrate that ROS-induced oxidative damage to SBPase affects growth, development, and chloroplast biogenesis in Arabidopsis through inhibiting carbon assimilation efficiency. The data presented here provide a case study that such inactivation of SBPase caused by carbonyl modification may be a kind of adaptation for plants to restrict the operation of the reductive pentose phosphate pathway under stress conditions.

关键词： SBPase carbonyl modification oxidative stress carbon assimilation starch accumulation growth inhibition.

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carbon assimilation characteristics of plants in oasis-desert ecotone and their response to CO2 enrichment

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Science China Earth Sciences 2004年第S1期 39-49页

作者： SU Peixi, CHEN Huaishun, AN Lizhe & LIU XinminCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

Six species of more than 20-year-old desert woody plants in the oasis-desert ecotone were selected for study. The results showed that: (1) in different growing seasons δ13C values of assimilating organ varied between -14‰ and -16‰ for Haloxylon ammodendron (HA),-14‰-15‰ for Calligonum mongolicum (CM) and-25‰- -28‰ for Caragana korshinskii(CK), Nitraria sphaerocarpa (NS) and Hedysarum scoparium (HS). (2) The net photosynthetic rate (Pn) of HA and CM was significantly higher than those of the other species. With the decrease in Pn for the six species, their intercellular CO2 concentration increased, but stomatal limitation value decreased under the intensive light. At the same time, the photochemical efficiency of PS II dropped to different degrees. (3) The CO2 enrichment experiment demonstrated that, Pn of HA and CM increased to different extent under 450 umol/mol, but their Pn reduced or approximated to the current condition under 650 umol/mol. Under 450 umol/mol the efficiency for solar energy u

关键词： oasis-desert ecotone carbon assimilation stable carbon isotope ratio photoinhibition CO2 enrichment.

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Arabidopsis tic62 trol Mutant Lacking Thylakoid- Bound Ferredoxin-NADP＋ Oxidoreductase Shows Distinct Metabolic Phenotype

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Molecular Plant 2014年第1期7卷 45-57页

作者： Minna Lintala Natalie Schuck Ina Thormaihlen Andreas Jungfer Katrin L. Weber Andreas P.M. Weber Peter Geigenberger Juirgen Soil Bettina Bolter Paula Mulo Molecular Plant Biology Department of Biochemistry University of Turku FI-20014 Turku Finland Munich Center for Integrated Protein Science CiPSM Ludwig-Maximilians-Universitat Munchen Feodor-Lynen-Strasse 25 D-81377 Munich Germany Department of Biology I Botany Ludwig-Maximilians-Universitat Munchen Groβhaderner Strasse 2-4 D-82152 Planegg-Martinsried Germany Department of Biology I Plant Metabolism Group Ludwig-Maximilians-Universitat Munchen Groβhaderner Strasse 2-4 D-82152 Planegg-Martinsried Germany Institute for Plant Biochemistry Cluster of Excellence on Plant Sciences (CEPLAS) Universitatsstrasse 1 D-40225 Dusseldorf Germany

Ferredoxin-NADP＋ oxidoreductase （FNR）, functioning in the last step of the photosynthetic electron transfer chain, exists both as a soluble protein in the chloroplast stroma and tightly attached to chloroplast membranes. Surface plasmon resonance assays showed that the two FNR isoforms, LFNR1 and LFNR2, are bound to the thylakoid membrane via the C-terminal domains of Tic62 and TROL proteins in a pH-dependent manner. The tic62 trol double mutants contained a reduced level of FNR, exclusively found in the soluble stroma. Although the mutant plants showed no visual phenotype or defects in the function of photosystems under any conditions studied, a low ratio of NADPH/NADP~ was detected. Since the CO2 fixation capacity did not differ between the tic62 trol plants and wild-type, it seems that the plants are able to funnel reducing power to most crucial reactions to ensure survival and fitness of the plants. However, the activity of malate dehydrogenase was down-regulated in the mutant plants. Apparently, the plastid metabolism is able to cope with substantial changes in directing the electrons from the light reactions to stromal metabolism and thus only few differences are visible in steady-state metabolite pool sizes of the tic62 trol plants.

关键词： carbon assimilation ferredoxin-NADP+ oxidoreductase photosynthesis chloroplast stromal metabolism Tic62 TROL.

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Estimation of food composition of Hodotermes mossambicus （Isoptera： Hodotermitidae） based on observations and stable carbon isotope ratios

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Insect Science 2011年第2期18卷 175-180页

作者： Craig T. Symes Stephan Woodborne School of Animal Plant and Environmental Sciences University of the Witwatersrand Wits Natural Resources and the Environment CSIR Pretoria South Africa

The diet of the harvester termite Hodotermes mossambicus was investigated at two sites with distinct dietary components： C4 grasses （δ13 C isotope values, -13.8%0 to -14.0%0） and C3 plants （δ13C isotope values, -25.6%0 to -27.1%0）. By comparing observations of food items carried into the colony by the termites and carbon isotope ratios of whole termites （that determined assimilated carbon）, the relative proportion of the C3 and C4 plant food components of the termite diet was estimated. There was agreement between the observational data and stable carbon isotopic data, with grass representing approximately 93% of the diet ofH. mossambicus at two study sites （urban and rural） on the South African highveld. However, when correcting for mass of food items, that is, C3 and C4, carried by termites, the proportion of grass （C4） in the diet may be underestimated.

关键词： carbon assimilation colony harvester termite Suikerbosrand

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High aluminum concentration and initial establishment of Handroanthus impetiginosus : clues about an Al non-resistant species in Brazilian Cerrado

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Journal of Forestry Research 2020年第6期31卷 2075-2082页

作者： Ane Marcela das Chagas Mendon ca Jean Marcel Sousa Lira Ana Luiza de Oliveira Vilela Daniel Amorim Vieira Nayara Cristina de Melo Joao Paulo Rodrigues Alves Delfi no Barbosa Programa de Pós-Graduacao em Fisiologia Vegetal Departamento de BiologiaUniversidade Federal de LavrasLavrasMG 37200-000Brazil Programa de Pós-Graduacao em Ciências Ambientais Instituto de Ciências ExatasUniversidade Federal de AlfenasAlfenasMG 37130-001Brazil Programa de Pós-Graduacao em Fitotecnia Departamento de AgriculturaUniversidade Federal de LavrasLavrasMG 37200-000Brazil Instituto Federal de Educacao Ciência e Tecnologia do Pará BelémPA 66093-020Brazil

Cerrado soils are acidic and nutrient-poor,with high content of solubilized Al 3+.Plants growing in these conditions may display adaptations to cope with high aluminum concentrations especially during early developmental *** investigated leaf nutritional status,and photosynthetic and growth characteristics during the initial establishment of Handroanthus impetiginosus(*** Dc.)Mattos,a secondary tree species distributed in the Brazilian *** goal was to understand leaf-level traits related to diff erent aluminum *** plants were cultivated in four diff erent aluminum sulfate concentrations:0,1,2,and 4 mM Al,for 40 *** performed analyses of growth,leaf gas exchange,chloroplast pigment content,and leaf mineral *** observed a linear increase of Al leaf content as a function of Al concentration in the nutrient *** grown in 1 mM Al showed a remarkable increase of K leaf content,net photosynthesis,stomatal conductance,and transpiration,while in 4 mM Al there were reductions of N,P,and K contents,gas exchange characteristics,and *** did not have mechanisms of avoidance,compartmentalization,or resistance to high Al ***,this species showed a hormetic response,with low Al concentrations stimulating and high Al concentrations inhibiting plant responses.

关键词： Hormesis Low Al concentration Al toxicity Cerrado species carbon assimilation

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Photosynthetic Performance and Potency of <i>Cannabis sativa</i>L. Grown under LED and HPS Illumination

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Agricultural Sciences 2021年第3期12卷 293-304页

作者： Michael W. Jenkins Curtis B. Livesay Rubisco Research and Consulting Sacramento USA Oaksterdam University Oakland USA Dynamite Ag Inc.

Light-emitting diodes (LEDs) can be used as an energy efficient alternative to high-pressure sodium (HPS), which have historically been the standard for supplemental lighting in cannabis cultivation. However, there is a lack of scientific understanding in the cannabis industry regarding plant physiology, which has resulted in the adoption of cannabis cultivation methods based on hearsay rather than scientific research. The goals of this study were to 1) compare LED lighting options that are commonly used in the cannabis industry and 2) compare the top performing LED light with an industry standard HPS light. Specifically, three LED lights were compared (California Light Works ((SolarSystem 1100), BIOS Lighting (Icarus Gi2), and Fluence Bioengineering (now Fluence by Osram) (SPYDR xPLUS)), based on light distribution, leaf temperature, and photosynthetic performance indices. The LED versus HPS comparison was based on light response curves measured at photosynthetic photon flux densities (PPFD) of (0, 100, 200, 300, 400, 500, 750, 1000, 1250, 1500, 1750 and 2000 μmol∙m−2∙s−1), carbon assimilation rates (A) μmol CO2 m−2∙s−1 using a LiCor-6800 and resulting cannabinoid potency (THCA). The SPYDR xPLUS-Fluence by Osram had the highest performing LED light used in the LED comparison. At the suggested distance from bulb to canopy in the HPS versus LED comparison (6 inches for LEDs and 4 ft for HPS), carbon assimilation rates displayed a 142% percent increase in plants grown under LED vs. HPS with average pho

关键词： Cannabis Light-Emitting Diode LED High-Pressure Sodium HPS carbon assimilation Light Response Curve Cannabinoid THC Potency

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