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canine herpesvirus seroprevalence and Associated Factors in Dogs of Mexico

Open Journal of Veterinary Medicine 2016年第10期6卷 149-162页

作者： Edgar Guillermo Valdivia Lara Jesica Ileana Ángeles Solis Cesar Cuenca Verde Juan Antonio Montaraz Crespo Laura Cobos Marín Juan Carlos Del Río García Guillermo Valdivia Anda Laboratorio de Patogenicidad Microbiana Unidad de Investigación Multidisciplinaria Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Cuautitlan Izcalli México Laboratorio de Inmunología Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Ciudad de México México Facultad de Medicina Veterinaria Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Laboratorio de Micotoxinas Unidad de Investigación Multidisciplinaria Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Ciudad de México México

canine herpesvirus (CHV-1) causes disease associated with high mortality in infect-ed puppies, which represents large financial losses for dog breeders. Since CHV-1 at the time of the study he had not been reported in Mexico, the main objective of this study was to determine the prevalence of antibodies against CHV-1 in canine kennels in the metropolitan area of Mexico City. A commercial enzyme-linked immuno-sorbent assay (ELISA) was used, and the results were compared to those of a viral neutralization test. The ELISA kit uses the complete viral particle as the antigen. The plaque reduction neutralization test was combined with the immunoperoxidase technique because of the low cytopathic effect of CHV-1. Neutralizing antibodies were also detected in 20 randomly selected samples. The prevalence of CHV-1 with ELISA was 87%. The concordance between ELISA and serum neutralization (SN) was 0.1129, the sensitivity of the ELISA against SN was 1.0 (100%), the positive predic-tive value was 0.39 (39%), and the negative predictive value was 1 (100%). These results show that ELISA is useful for monitoring the dog population for CHV-1;a positive test result requires confirmation with an SN test, and a negative ELISA result indicates a high probability of being SN-negative. The only variables that were sta-tistically associated with CHV-1 prevalence were breed and kennel. A statistically significant relationship between the degree of ELISA and SN titer was obtained, with a confidence level of 95%. None of the clinical presentation factors was statistically significant. These results suggest that most of the canine population studied in Mex-ico is in a herpesvirus latency state.

关键词： canine herpesvirus seroprevalence Enzyme-Linked Immunosorbent Assay Serum Neutralization Test

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