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campylobacterjejuni in Musca domestica： An examination of survival and transmission potential in light of the innate immune responses of the house flies

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Insect Science 2017年第4期24卷 584-598页

作者： Carson Gill Simon Bahrndorff Carl Lowenberger Department of Biological Sciences Simon Fraser University 8888 University Drive Burnaby BC Canada National Food Institute Technical University of Denmark 2800 Kongens Lyngby Denmark

The house fly, Musca domestica, has been implicated as a vector of Campy- lobacter spp., a major cause of human disease. Little is known whether house flies serve as biological amplifying hosts or mechanical vectors for campylobacterjejuni. We in- vestigated the period after C. jejuni had been ingested by house flies in which viable C. jejuni colonies could be isolated from whole bodies, the vomitus and the excreta of adult M. domestica and evaluated the activation of innate immune responses of house flies to ingested C. jejuni over time. C. jejuni could be cultured from infected houseflies soon after ingestion but no countable C. jejuni colonies were observed 〉 24 h postingestion. We detected viable C. jejuni in house fly vomitus and excreta up to 4 h after ingestion, but no viable bacteria were detected 〉 8 h. Suppression subtractive hybridization identi- fied pathogen-induced gene expression in the intestinal tracts of adult house flies 4-24 h after ingesting C. jejuni. We measured the expression of immune regulatory （thor, JNK, and spheroide） and effector （cecropin, diptericin, attacin, defensing, and lysozyme） genes in C. jejuni-infected and -uninfected house flies using quantitative real time PCR. Some house fly factor, or combination of factors, eliminates C. jejuni within 24 h postingestion. Because C. jejuni is not amplified within the body of the housefly, this insect likely serves as a mechanical vector rather than as a true biological, amplifying vector for C. jejuni, and adds to our understanding of insect pathogen interactions.

关键词： antimicrobial peptides campylobacter spp. campylobacteriosis house fly innate immunity Musca domestica suppression subtractive hybridization （SSH） vector

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